Search results for "TLE"
showing 10 items of 2417 documents
Immobilisation of yeasts on oak chips or cellulose powder for use in bottle-fermented sparkling wine
2019
[EN] Sparkling wine production comprises two successive fermentations performed by Sacharomyces cerevisiae strains. This research aimed to: develop yeast immobilisation processes on two wine-compatible supports; study the effects of yeast type (IOC 18-2007 and 55A) and the immobilisation support type (oak chips and cellulose powder) on the fermentation kinetics, the deposition rate of lees and the volatile composition of the finished sparkling wine; compare the fermentation parameters of the wines inoculated with immobilised or non-immobilised cells. Proper immobilisation of yeast on oak chips and cellulose powder was demonstrated by electron microscopy. Total sugar consumption occurred in …
Effects of distance and scale dependence in geographical models of cities and territories
2019
International audience; This third chapter questions the essential role of distance and scale dependence in models to analyzing the spatial configuration of human settlements and activities, as well as the processes that lead to their evolution, with reference to urban or regional study areas. The subject of study therefore concerns both the form of human settlements and the actions of people (individuals, groups) in their territory (or territories). A human settlement consists of the materialization in space-the physical inscription-of one or more human activities. It is characterized by a certain degree of sustainability. It can be a group of a few buildings or a larger group of thousands…
Synthetic Haptens and Monoclonal Antibodies to the Cyanotoxin Anatoxin‐a
2019
Early warning systems for monitoring toxic events may benefit from the availability of monoclonal antibodies enabling the sensitive and specific detection of anatoxin-a, a cyanotoxin involved in numerous cases of animal poisoning resulting from toxic algal blooms in freshwaters. Through the synthesis of three functionalized derivatives of anatoxin-a, we have succeeded in generating the first-ever reported immunoreagents (bioconjugates and antibodies) suitable for the development of immunoanalytical approaches aimed at rapid and onsite detection of this harmful cyanotoxin.
Identification and quantification of phosphatidylcholines containing very-long-chain polyunsaturated fatty acid in bovine and human retina using liqu…
2010
The retina is one of the vertebrate tissues with the highest content in polyunsaturated fatty acids (PUFA). A large proportion of retinal phospholipids, especially those found in photoreceptor membranes, are dipolyunsaturated molecular species. Among them, dipolyunsaturated phosphatidylcholine (PC) molecular species are known to contain very-long-chain polyunsaturated fatty acids (VLC-PUFA) from the n-3 and n-6 series having 24-36 carbon atoms (C24-C36) and four to six double bonds. Recent interest in the role played by VLC-PUFA arose from the findings that a protein called elongation of very-long-chain fatty acids 4 (ELOVL4) is involved in their biosynthesis and that mutations in the ELOVL…
Determination of macrolide antibiotics in meat and fish using pressurized liquid extraction and liquid chromatography–mass spectrometry
2008
We developed a method for determining the quantities of seven macrolide antibiotics in meat and fish by using pressurized liquid extraction (PLE) and liquid chromatography-mass spectrometry with electrospray ionization (LC-(ESI)MS). The PLE was optimized with regard to solvents, temperature, pressure, extraction time and number of cycles. The optimum conditions were: methanol as the extraction solvent; a temperature of 80 degrees C; a pressure of 1500psi; an extraction time of 15min; 2 cycles; a flush volume of 150% and a purge time of 300s. All recoveries for macrolide antibiotics were over 77% at 200mug/kg, except for erythromycin, which was 58%. The repeatability and reproducibility on d…
Direct infusion mass spectrometry as a fingerprint of protein-binding media used in works of art
2005
A direct infusion mass spectrometry method for the characterization of proteinaceous glues from binding media used in pictorial works of art prior to conservation or restoration treatment is proposed. Amino acids are released by acid hydrolysis and dissolved in a mixture of acidic water and ethanol. This mixture is directly infused into a mass spectrometer without any derivatization. The mass spectrometer is operated in positive ion electrospray mode (ESI-MS) to yield [M+H]+ ions for the amino acids. Relative amounts of each amino acid are calculated for each protein (beef and porcine gelatines, albumin, casein and egg). The analyzed proteins were satisfactorily distinguished. The method is…
Garnet and spinel lherzolite assemblages in MgO-Al2O3-SiO2 and CaO-MgO-Al2O3-SiO2: thermodynamic models and an experimental conflict
2012
The recent publication of an updated thermodynamic dataset for petrological calculations provides an opportunity to illustrate the relationship between experimental data and the dataset, in the context of a new set of activity–composition models for several key minerals. These models represent orthopyroxene, clinopyroxene and garnet in the system CaO–MgO–Al2O3–SiO2 (CMAS), and are valid up to 50 kbar and at least 1800 °C; they are the first high-temperature models for these phases to be developed for the Holland & Powell dataset. The models are calibrated with reference to phase-relation data in the subsystems CaO–MgO–SiO2 (CMS) and MgO–Al2O3–SiO2 (MAS), and will themselves form the basis o…
Nesting of the Spotless Starling, <em>Sturnus unicolor</em>, on the island of Favignana (Aegadian Islands, Sicily)
2021
The presence of the Spotless Starling in Sicily was reported for the first time at the beginning of the XIX century, although the first nests for the province of Trapani are reported almost at the end of the XX century. As a breeder, the Spotless Starling remained absent from all the circumsicilian islands until the first report for the island of Pantelleria, in 2012. In 2020, the nest of Spotless Starling was observed on the island of Favignana, within a hole on an antenna. The couple fed the chicks with Ligia italica crustacean isopod of the family Ligiidae. The nesting on the island of Favignana confirms the continuous expansion of the species in Sicily.
Preliminary Results on the Effect of Magnetic Fields on Anther Culture and Pollen Germination of Citrus clementina Hort. ex Tan.
2003
Exposure to electromagnetic fields seems to modify the biological behaviour of seeds, roots, pollen grains and buds of several plants. This procedure has been also suggested as a tool to improve the utilization of plant species (Firetto et al., 2000, 2001, Petruszewski S., 1996). Since the first haploid embryogenic calli production and plantlet regeneration by anther culture of C. clementina Hort. ex Tan. 'Nules' was reported (Germany et al., 1994), several studies have been carried out to improve both the frequency of microspore embryogenesis and the percentage of plantlet regeneration. To enlarge the number of respondent genotypes and to improve the induction rate (the frequency of pollen…
Physical Fitness Evaluation of School Children in Southern Italy: A Cross Sectional Evaluation
2018
The aim of this work was to evaluate the fitness levels of different physical components in schoolchildren in southern Italy and identify age-related effects of physical performance. One hundred and fifty-four schoolchildren with ages ranging between 6 and 10 years (age 8.1 ± 1.45 years; 33.70 ± 10.25 kg; 131.50 ± 13.60 cm) were recruited for the investigation. Each scholar underwent a fitness-test battery composed of five elements. A Hand-Grip Strength Test to assess the strength of the hand muscles, a Standing Broad Jump Test to assess lower body explosive strength, a Sit-Up Test to exhaustion to evaluate abdominal muscular endurance, a 4 × 10-m Shuttle Run Test to assess agility, and a 2…