Search results for "TLE"

Comparison of two PCR methods for detection of Leptospira interrogans in formalin-fixed and paraffin-embedded tissues

2012

In this study we compared two polymerase chain reaction (PCR) methods using either 16S ribosomal RNA (rRNA) or 23S rRNA gene primers for the detection of different Leptospira interrogans serovars. The performance of these two methods was assessed using DNA extracted from bovine tissues previously inoculated with several bacterial suspensions. PCR was performed on the same tissues before and after the formalin-fixed, paraffin-embedding procedure (FFPE tissues). The 23S rDNA PCR detected all fresh and FFPE positive tissues while the 16S rDNA-based protocol detected primarily the positive fresh tissues. Both methods are specific for pathogenic L. interrogans. The 23S-based PCR method successfu…

Molecular relationship among Salmonella dublin isolates identified at the Center for Enterobacteriaceae of Palermo during the years 1971-85.

1987

SUMMARYA molecular epidemiological study was carried out on 60Salmonella dublinisolates identified at the Southern Italy Enterobacteriaceae Center between 1971 and 1985. These included 23 isolates from children with diarrhoea in Palermo obtained during 1984.All isolates from the outbreak of gastroenteritis in children were resistant to chloramphenicol and streptomycin and harboured two plasmids of 50 MDa and 3 MDa molecular weight, whereas the majority of the isolates identified before 1984 were susceptible to these antibiotics and carried only a 50 MDa molecular weight plasmid. FourS. dublinstrains successively identified from cattle (Palermo, Foggia, Portici) and from a child (Palermo) we…

Methicillin-resistant Staphylococcus pseudintermedius as causative agent of dairy cow mastitis.

2013

Staphylococcus pseudintermedius is a coagulase-positive specie similar to Staphylococcus intermedius , frequently associated with pyoderma, otitis and urinary tract infections of dogs and cats (van Duijkeren and others 2011). No information about bovine mastitis caused by S pseudintermedius is available in the literature. Antimicrobial resistance among S pseudintermedius strains is increasing: in the past, susceptibility to most antibiotics was common (Bond and Loeffler 2012), but in the last few years methicillin-resistant S pseudintermedius (MRSP) strains have emerged as a significant animal health problem in veterinary medicine (Schwarz and others 2008, van Duijkeren and others 2008, Wee…

PCR-based procedures for detection and quantification of Staphylococcus aureus and their application in food.

2006

Aims:  To evaluate the specificity of nuc targeted primers for PCR detection of Staphylococcus aureus in different food matrices and to establish a RTQ-PCR procedure suitable for the routine detection and quantification of this pathogen in food. Methods and Results:  Specificity of nuc targeted primers (Pri1–Pri2 and the newly designed RTQ-PCR primers) was tested on a total of 157 strains of genetically confirmed identity, including reference and food isolates. PCR detection on artificially inoculated beef samples by DNA extraction using a DNeasy Tissue Kit (Qiagen GmhH, Hilden, Germany) showed a sensitivity value around 103 CFU g−1. The two RTQ-PCR systems, incorporating SYBR-Green I and T…

Microbiological quality of carbonated drinking water produced with in-home carbonation systems.

2005

The microbiological quality of carbonated water produced with tap water in commercial in-home carbonation systems was determined, the results being discussed in the context of the microbiological quality of the tap water used, the properties of the drink makers, and the procedures of preparation and washing of various parts of the appliance. The last-mentioned data were received from each participant of the study by questionnaire. Escherichia coli, coliforms, fecal streptococci and spore-forming sulphite-reducing anaerobes were used as indicators for the hygienic quality of the water. Tap-water samples were collected according to the usual procedure when filling the carbonating bottle, i.e.…

Tumor necrosis factor-alpha converting enzyme is processed by proprotein-convertases to its mature form which is degraded upon phorbol ester stimulat…

2003

Tumor necrosis factor-alpha converting enzyme (TACE or ADAM17) is a member of the ADAM (a disintegrin and metalloproteinase) family of type I membrane proteins and mediates the ectodomain shedding of various membrane-anchored signaling and adhesion proteins. TACE is synthesized as an inactive zymogen, which is subsequently proteolytically processed to the catalytically active form. We have identified the proprotein-convertases PC7 and furin to be involved in maturation of TACE. This maturation is negatively influenced by the phorbol ester phorbol-12-myristate-13-acetate (PMA), which decreases the cellular amount of the mature form of TACE in PMA-treated HEK293 and SH-SY5Y cells. Furthermore…

Los efectos de la adversidad climática y sanitaria sobre la agricultura y la sociedad castellonense del siglo XVIII

2017

El presente artículo recurre a la microhistoria para analizar los archivos municipales de la Plana de Castelló, con el objetivo de obtener nuevas referencias sobre la evolución climática de la fachada mediterránea peninsular durante un periodo cronológico concreto: el siglo XVIII, enclavado en los estadios finales de la Pequeña Edad de Hielo. Con esta finalidad, se abordarán las principales sequías, precipitaciones extremas, plagas y epidemias acontecidas en la Plana de Castelló durante el Setecientos, y aquellos recursos, tanto religiosos como seculares, utilizados para prevenir, primero, y erradicar, después, las adversidades climáticas, biológicas y sanitarias. This paper discusses a mic…

Using the concept of spatial contexts for the prediction of archaeological rural settlement

2011

Unusually high frequency natural VLF radio emissions observed during daytime in Northern Finland

2016

Geomagnetic field variations and electromagnetic waves of different frequencies are ever present in the Earth's environment in which the Earth's fauna and flora have evolved and live. These waves are a very useful tool for studying and exploring the physics of plasma processes occurring in the magnetosphere and ionosphere. Here we present ground-based observations of natural electromagnetic emissions of magnetospheric origin at very low frequency (VLF, 3–30 kHz), which are neither heard nor seen in their spectrograms because they are hidden by strong impulsive signals (sferics) originating in lightning discharges. After filtering out the sferics, peculiar emissions are revealed in these dig…

In vitro comparison of the retention capacity of new aesthetic brackets.

1999

Tensile bond strength and bond failure location were evaluated in vitro for two types of aesthetic brackets (non-silanated ceramic, polycarbonate) and one stainless steel bracket, using bovine teeth as the substrate and diacrylate resin as the adhesive. The results show that metallic bracket had the highest bond strength (13.21 N) followed by the new plastic bracket (12.01 N), which does not require the use of a primer. The non-silanated ceramic bracket produced the lowest bond strength (8.88 N). Bond failures occurred mainly between bracket and cement, although a small percentage occurred between the enamel-cement interface with the metal and plastic brackets and within the cement for the …