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showing 10 items of 17685 documents
Engineering CRISPR guide RNA riboswitches for in vivo applications
2019
CRISPR-based genome editing provides a simple and scalable toolbox for a variety of therapeutic and biotechnology applications. Whilst the fundamental properties of CRISPR proved easily transferable from the native prokaryotic hosts to eukaryotic and multicellular organisms, the tight control of the CRISPR-editing activity remains a major challenge. Here we summarise recent developments of CRISPR and riboswitch technologies and recommend novel functionalised synthetic-gRNA (sgRNA) designs to achieve inducible and spatiotemporal regulation of CRISPR-based genetic editors in response to cellular or extracellular stimuli. We believe that future advances of these tools will have major implicati…
Bacterioplankton dynamics driven by interannual and spatial variation in diatom and dinoflagellate spring bloom communities in the Baltic Sea
2020
17 pages, 6 figures, 2 tables, supporting information https://doi.org/10.1002/lno.11601.-- This is the pre-peer reviewed version of the following article: María Teresa Camarena‐Gómez, Clara Ruiz‐González, Jonna Piiparinen, Tobias Lipsewers, Cristina Sobrino, Ramiro Logares, Kristian Spilling, Bacterioplankton dynamics driven by interannual and spatial variation in diatom and dinoflagellate spring bloom communities in the Baltic Sea, Limnology and Oceanography 66(1): 255-271 (2021), which has been published in final form at https://doi.org/10.1002/lno.11601. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions
Glycymeris pilosa (Bivalvia) - A high-potential geochemical archive of the environmental variability in the Adriatic Sea.
2019
Due to its outstanding longevity (decades), the shallow-water bivalve Glycmeris pilosa represents a prime target for sclerochronological research in the Mediterranean Sea. In the present study, we analyzed the microgrowth patterns and the stable carbon (δ13Cshell) and oxygen (δ18Oshell) isotopes of the outer shell layer of live-collected G. pilosa specimens from four different sites along the Croatian coast, middle Adriatic Sea. Combined analysis of shell growth patterns and temporally aligned δ18Oshell data indicated that the main growing season lasts from April to December, with fastest growth rates occurring during July and August when seawater temperatures exceeded 22 °C. Slow growth in…
Carbon metabolic rates and GHG emissions in different wetland types of the Ebro Delta
2020
Deltaic wetlands are highly productive ecosystems, which characteristically can act as C-sinks. However, they are among the most threatened ecosystems, being very vulnerable to global change, and require special attention towards its conservation. Knowing their climate change mitigating potential, conservation measures should also be oriented with a climatic approach, to strengthen their regulatory services. In this work we studied the carbon biogeochemistry and the specific relevance of certain microbial guilds on carbon metabolisms of the three main types of deltaic wetlands located in the Ebro Delta, north-eastern Spain, as well as how they deal with human pressures and climate change ef…
Effects of traditional coppice practices and microsite conditions on tree health in a European beech forest at its southernmost range
2016
Abstract: European beech (Fagus sylvatica) grows at the southern limit of its range in the mountain-Mediterranean vegetation belt up to the timberline. The southernmost beech forests of Sicily (southern Italy) show peculiar ecological, structural and silvicultural characteristics, growing in fragmented and isolated stands near the timberline and in topographically marginal unfavorable habitats. Past silvicultural practices increased the heterogeneity of stand structure at these sites. We compared stand structural characteristics and tree health in coppice-cut and control beech stands with respect to the local topographic gradient (bottom, slope and ridge) and canopy cover (clearing/border v…
Effects of Arbuscular Mycorrhizal Fungi on the Vegetative Vigor of Ailanthus altissima (Mill.) Swingle Seedlings under Sustained Pot Limitation
2018
In order to invade new ecosystems, invasive alien plants need to cope with different microbial communities. Whilst the ability to avoid antagonists is well recognized, the opportunity to establish mutualistic associations is less known, even in widespread invasive species such as Ailanthus altissima (Mill.) Swingle. We sought to evaluate whether the beneficial effects of arbuscular mycorrhizal fungi (AMF) on Ailanthus seedlings are maintained over time, under prolonged pot limitation. We compared three-month-, three-year- and four-year-old mycorrhizal seedlings grown in natural forest soil (NT) with seedlings grown in sterilized (ST) and non-mycorrhizal (NM) soils, in pots of 3.4 L (22 &tim…
Vegetated Habitats Trophically Support Early Development Stages of a Marine Migrant Fish in a Coastal Lagoon
2020
Understanding the trophic ecology of early developmental stages of commercial fish species migrating between sea and coastal lagoons is crucial to effective management of nursery habitats and resulting resources. While most information on diet shift of the gilthead seabream Sparus aurata comes from studies in experimental conditions, here we investigated the trophic niche dynamics of post-larvae, juveniles and sub-adults in natural environment, hypothesising that habitat and resources use of marine and lagoonal sites will exhibit strong relationship with ontogeny of the species. Carbon and nitrogen stable isotopes showed evidence of a clear shift in the trophic niche features, trophic posit…
Small-scale variability in geomorphological settings influences mangrove-derived organic matter export in a tropical bay
2017
Abstract. Organic matter (OM) exchanges between adjacent habitats affect the dynamics and functioning of coastal systems, as well as the role of the different primary producers as energy and nutrient sources in food webs. Elemental (C, N, C : N) and isotope (δ13C) signatures and fatty acid (FA) profiles were used to assess the influence of geomorphological setting in two climatic seasons on the export and fate of mangrove OM across a tidally influenced tropical area, Gazi Bay (Kenya). The main results indicate that tidal transport, along with riverine runoff, plays a significant role in the distribution of mangrove organic matter. In particular, a marked spatial variability in the export of…
Trophic flexibility of the Atlantic blue crab Callinectes sapidus in invaded coastal systems of the Apulia region (SE Italy): A stable isotope analys…
2017
Abstract The Atlantic blue crab Callinectes sapidus is recognized as an Invasive Alien Species in the Mediterranean Sea. However, its trophic role and feeding flexibility in invaded benthic food webs have been addressed only recently. Here, field samplings were conducted in winter and summer in five coastal systems of the Apulia region (SE Italy), three located on the Ionian Sea (Mar Piccolo, Torre Colimena, and Spunderati) and two on the Adriatic Sea (Acquatina and Alimini Grande). Captured blue crabs were weighed and had their δ 13 C and δ 15 N isotopic signatures measured; their trophic level (TL) was estimated using the mussel Mytilus galloprovincialis as isotopic baseline. C. sapidus a…
Ecological effects of aquaculture on living and non-living suspended fractions of the water column: a meta-analysis.
2006
The effects of aquaculture on the ecology of the water column have been extensively studied in the last two decades. However, to date, it has not been possible to extrapolate homogeneous information from the peer-reviewed literature. In the present study, 68 peer-reviewed articles were analysed and about 1087 study cases were used to test whether worldwide cultivations of aquatic organisms (shrimps, fish, bivalves and polyculture) have a differential effect on living and non-living fractions of the water column (suspended matter, chlorophyll-a, particulate organic carbon, nitrogen and phosphorus, bacteria and plankton). Meta-analysis feasibility depends on obtaining an estimate of the effec…