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RESEARCH PRODUCT
Engineering CRISPR guide RNA riboswitches for in vivo applications
Roberto GaliziAlfonso JaramilloAlfonso JaramilloAlfonso Jaramillosubject
0106 biological sciencesRiboswitchComputer scienceGenetic enhancementBiomedical EngineeringBioengineeringComputational biology01 natural sciences03 medical and health sciencesSynthetic biologyGenome editing010608 biotechnologyHumansCRISPRClustered Regularly Interspaced Short Palindromic RepeatsGuide RNAQH426030304 developmental biologyGene Editing0303 health sciencesReproducibility of ResultsRNAMulticellular organismRiboswitchGenetic EngineeringRNA Guide KinetoplastidaBiotechnologydescription
CRISPR-based genome editing provides a simple and scalable toolbox for a variety of therapeutic and biotechnology applications. Whilst the fundamental properties of CRISPR proved easily transferable from the native prokaryotic hosts to eukaryotic and multicellular organisms, the tight control of the CRISPR-editing activity remains a major challenge. Here we summarise recent developments of CRISPR and riboswitch technologies and recommend novel functionalised synthetic-gRNA (sgRNA) designs to achieve inducible and spatiotemporal regulation of CRISPR-based genetic editors in response to cellular or extracellular stimuli. We believe that future advances of these tools will have major implications for both basic and applied research, spanning from fundamental genetic studies and synthetic biology to genetic editing and gene therapy.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2019-02-01 | Current Opinion in Biotechnology |