Search results for "TRF"
showing 6 items of 6 documents
Differentially Expressed tRNA-Derived Small RNAs Co-Sediment Primarily with Non-Polysomal Fractions in Drosophila
2017
Recent studies point to the existence of poorly characterized small regulatory RNAs generated from mRNAs, rRNAs and tRNAs. To explore the subcellular location of tRNA-derived small RNAs, 0–1 and 7–8 h Drosophila embryos were fractionated on sucrose density gradients. Analysis of 12,553,921 deep-sequencing reads from unfractionated and fractionated Drosophila embryos has revealed that tRFs, which are detected mainly from the 5’ends of tRNAs, co-sediment with the non-polysomal fractions. Interestingly, the expression levels of a subset of tRFs change temporally following thematernal-to-zygotic transition in embryos. We detected non-polysomal association of tRFs in S2 cells as well. Differenti…
LKS-92 Coordinates Transformation to ITRF2000
2014
LKS-92 is Latvian geodetic coordinate system used as an EUREF89 realization in Latvia. LKS-92 is official coordinate system for procuring of essential geospatial data. For aeronautical and other purposes ITRF2000 is used in publication of geospatial data. Research is done to obtain transformation parameters from LKS-92 realization epoch 1992.75 through LatPos to ITRF2000 epoch 2000.00. Valuation of two different coordinate adjustment strategies and accuracy of parameters is done. Results of research could be used for transformation from LKS-92 to ITRF2000 for all kind of geospatial data.
Cancer cells induce immune escape via glycocalyx changes controlled by the telomeric protein TRF2
2019
International audience; Myeloid-derived suppressor cells (MDSCs) are immature myeloid cells with strong immunosuppressive activity that promote tumor growth. In this study, we describe a mechanism by which cancer cells control MDSCs in human cancers by upregulating TRF2, a protein required for telomere stability. Specifically, we showed that the TRF2 upregulation in cancer cells has extratelomeric roles in activating the expression of a network of genes involved in the biosynthesis of heparan sulfate proteoglycan, leading to profound changes in glycocalyx length and stiffness, as revealed by atomic force microscopy. This TRF2-dependent regulation facilitated the recruitment of MDSCs, their …
unitas: the universal tool for annotation of small RNAs
2017
AbstractBackgroundNext generation sequencing is a key technique in small RNA biology research that has led to the discovery of functionally different classes of small non-coding RNAs in the past years. However, reliable annotation of the extensive amounts of small non-coding RNA data produced by high-throughput sequencing is time-consuming and requires robust bioinformatics expertise. Moreover, existing tools have a number of shortcomings including a lack of sensitivity under certain conditions, limited number of supported species or detectable sub-classes of small RNAs.ResultsHere we introduce unitas, an out-of-the-box ready software for complete annotation of small RNA sequence datasets, …
Ecological role of mycotoxins produced by Fusarium graminearum : consequences of the presence of deoxynivalenol (DON) in crop residues on the soil mi…
2012
Fusarium graminearum is a plant pathogenic fungus, causing devastating disease “Fusarium head blight” (FHB) in cereals including wheat and maize. It also contaminates the grains with mycotoxins including deoxynivalenol (DON) which are toxic to human and animals. This disease has resulted in the serious losses in grain yield and quality. We established through a first bibliographic review that during off season fungus survives saprophytically on the crop residues (ecological habitat) and serves as primary inoculum for the next season crop. However, we noticed also that the literature was poor about the role mycotoxins could play in the establishment of F. graminearum in such a habitat. The m…
SULL'IMPIEGO SPERIMENTALE DI UN COLLANTE BIOLOGICO NELLE ANASTOMOSI INTESTINALI NEL RATTO IN ACCRESCIMENTO
1980
The problems that arise in the preparation of the anastomoses of channelicular organs have not found a unique solution. In fact, when it is necessary to proceed to the synthesis of very small gauge hollow organs as in the accreting subjects (infants) the risk of formation of a stenosis is always present despite the various techniques practiced. In recent years several experimental researches have been carried out in order to verify the validity of biological adhesives in intestinal anastomoses in rapidly growing animals. For this purpose we have chosen the rat whose rapidity of somatic growth permits an evaluation in times contained