Search results for "TRIF"
showing 10 items of 1419 documents
Shortcut nitrification-denitrification by means of autochthonous halophilic biomass in an SBR treating fish-canning wastewater
2017
Abstract Autochthonous halophilic biomass was cultivated in a sequencing batch reactor (SBR) aimed at analyzing the potential use of autochthonous halophilic activated sludge in treating saline industrial wastewater. Despite the high salt concentration (30 g NaCl L −1 ), biological oxygen demand (BOD) and total suspended solids (TSS), removal efficiencies were higher than 90%. More than 95% of the nitrogen was removed via a shortcut nitrification-denitrification process. Both the autotrophic and heterotrophic biomass samples exhibited high biological activity. The use of autochthonous halophilic biomass led to high-quality effluent and helped to manage the issues related to nitrogen removal…
Mathematical modelling of greenhouse gas emissions from membrane bioreactors: A comprehensive comparison of two mathematical models.
2018
Abstract This paper compares two mathematical models (Model I and Model II) to predict greenhouse gases emission from a University Cape Town (UCT) – membrane bioreactor (MBR) plant. Model I considers N 2 O production only during denitrification. Model II takes into account the ammonia-oxidizing bacteria (AOB) formation pathways for N 2 O. Both models were calibrated adopting real data. Model comparison was performed in terms of (i) sensitivity analysis (ii) best fit and (iii) model prediction uncertainty. On average 6% of factors of Model I and 9% of Model II resulted to be important. In terms of best fit, Model II had a better capability of reproducing the measured data. The average effici…
The effect of the solids and hydraulic retention time on moving bed membrane bioreactor performance
2018
Abstract The aim of the present paper was to investigate the effect of solids (SRT) and hydraulic (HRT) retention time on Integrated Fixed Film Activated Sludge (IFAS) University of Cape Town (UCT) membrane Bioreactor (MBR). In particular, three different pairs of SRT and HRT values were analysed, namely, Phase I 56 d/30 h, Phase II 31 d/15 h and Phase III 7 d/13 h. The short-term effect of these three SRT/HRT conditions was assessed by analysing several system performance indicators: organic carbon and biological nutrient (nitrogen and phosphorus) removal, biomass respiratory activity, activated sludge filtration properties and membrane fouling. The results showed that the decrease of SRT/…
Modelling denitrification including the dynamics of denitrifiers and their progressive ability to reduce nitrous oxide: comparison with batch experim…
2005
Nitrous oxide contributes to the global greenhouse effect and affects the chemistry of O 3 in the upper troposphere and lower stratosphere. To define a relevant model for microbial NO 3 - and N 2 O reductions in soil and estimate the parameters involved, we propose a method combining measurements of anaerobic soil slurry and simulations of NO 3 - and N 2 O reductions, including non-enzymatic competition between NO 3 - and N 2 O as electron acceptors and the microbial dynamics of two denitrifier groups that are either able or unable to reduce N 2 O. Three models varying in the description of soil capability to reduce N 2 O through denitrification were assessed. The procedure was applied on a…
Streamwater responses to reduced nitrogen deposition at four small upland catchments in Norway
2020
AbstractReduced emissions of nitrogen (N) in Europe have resulted in decreasing atmospheric deposition since 1990. Long-term data (1988–2017) from four small Norwegian catchments located along gradients in N deposition, rainfall, and organic carbon (C) show different responses to 25–30% reductions in N deposition during the same period. At three sites the decreased N deposition caused reduced leaching of nitrate to surface water, whereas the westernmost site showed no decrease, probably due to thin soils with low C:N ratio, poor vegetation cover and high precipitation. The loss of total N to streamwater constituted 30–50% of the N deposition. Losses via denitrification are unknown but assum…
Enhanced nitrogen removal of low carbon wastewater in denitrification bioreactors by utilizing industrial waste toward circular economy
2020
Abstract Aquaculture needs practical solutions for nutrient removal to achieve sustainable fish production. Passive denitrifying bioreactors may provide an ecological, low-cost and low-maintenance approach for wastewater nitrogen removal. However, innovative organic materials are needed to enhance nitrate removal from the low carbon effluents in intensive recirculating aquaculture systems (RAS). In this study, we tested three additional carbon sources, including biochar, dried Sphagnum sp. moss and industrial potato residues, to enhance the performance of woodchip bioreactors treating the low carbon RAS wastewater. We assessed nitrate (NO3−) removal and microbial community composition durin…
Comparison of Two Mathematical Models for Greenhouse Gas Emission from Membrane Bioreactors
2017
In this study two mathematical models (Model I and Model II), able to predict the nitrous oxide (N2O) and carbon dioxide (CO2) emission from an University Cape Town (UCT) – membrane bioreactor (MBR) plant, have been compared. Model I considers the N2O production only during the denitrification. Model II takes into account the two ammonia-oxidizing bacteria (AOB) formation pathways for N2O. Both models were calibrated adopting real data. Results highlight that Model II had a better capability of reproducing the measured data especially in terms of N2O model outputs. Indeed, the average efficiency related to the N2O model outputs was equal to 0.3 and 0.38 for Model I and Model II respectively.
Insights into the effect of soil pH on N(2)O and N(2) emissions and denitrifier community size and activity.
2010
ABSTRACT The objective of this study was to investigate how changes in soil pH affect the N 2 O and N 2 emissions, denitrification activity, and size of a denitrifier community. We established a field experiment, situated in a grassland area, which consisted of three treatments which were repeatedly amended with a KOH solution (alkaline soil), an H 2 SO 4 solution (acidic soil), or water (natural pH soil) over 10 months. At the site, we determined field N 2 O and N 2 emissions using the 15 N gas flux method and collected soil samples for the measurement of potential denitrification activity and quantification of the size of the denitrifying community by quantitative PCR of the narG , napA ,…
Characterization of denitrification gene clusters of soil bacteria via a metagenomic approach
2009
International audience; Denitrification is a microbial respiratory process contributing to the emission of greenhouse gas. The study of denitrifying bacteria, like that of others, is hindered by characteristics that can prevent up to 99% of soil bacteria from being cultivated in vitro. New approaches based on the direct extraction of DNA from the natural environment and PCR amplifications can overcome limitations due to bacterial unculturability, but until now their application to denitrification genes has led only to the recovery of partial sequences for some of these genes.Our goals in this study were to apply a metagenomic approach characterized by cloning of DNA extracted from soil and …
Relative involvement of nitrate and nitrite reduction in the competitiveness of Pseudomonas fluorescens in the rhizosphere of maize under non-limitin…
2002
Competition between different isogenic mutants of Pseudomonas fluorescens unable to carry out the first steps of the denitrification pathway was compared in soil micro-columns non-planted or planted with maize. A new isogenic mutant of P. fluorescens YT101 affected in both nitrate and nitrite respirations was constructed and used as a model of non-denitrifying strain (FM69MS strain). The outcome of the selection exerted by the plant after co-inoculation of FM69MS at the same ratio either with an isogenic denitrifier unable to reduce nitrate (Nar(-) mutant) or with an isogenic NO2 (-) accumulator (Nir(-) mutant) was investigated in non-limiting NO3 (-) conditions. Regardless of the inoculate…