Search results for "TRIF"

Fusarium proliferatum: induction and intracellular location of a lipoxygenase

1998

The fungus Fusarium proliferatum was grown in a soya oil- and glucose-supplemented medium. Induction of lipoxygenase activity (13-fold) in an extract of Fusarium biomass has been observed at day four on soya oil culture medium. A band at 232 kDa was detected using a specific lipoxygenase stain combined with native PAGE. The method of fungal homogenate obtained has been checked via subcellular marker enzymes activities: succinate dehydrogenase (SDH) for mitochondrial fraction and NADH-cytochrome C reductase (NADH-cyt. C) for the microsomal fraction. Protoplasts production and disintegration followed by subcellular fractionation using differential centrifugation was the best method to recover…

Chitin synthetase activity in Candida albicans: subcellular distribution in yeast cells and protoplasts

1991

Chitin synthetase activity was detected in a partly zymogenic form in cell-free homogenates obtained from C. albicans yeast cells and protoplasts of the same type of cells. By isopycnic centrifugation on sucrose gradients of the cell-free homogenates two fractions with chitin synthetase activity were obtained: one was associated with the plasma membrane (labelled with [ 3 H]concanavalin A (con A) and buoyant density 1·195 g ml −1 ) in a partly active state, and the second was in the cytoplasm, where the enzyme was in a particulate fully zymogenic form, lacking affinity to con A. The buoyant density of the enzyme found in this location depended on the method of cell breakage. Lysis of partly…

Numerical analysis of density gradient centrifugation profiles from eukaryotic DNA

1990

A numerical method for the deconvolution of superimposed Gaussian distributions with a unique solution has been proposed by Medgyessy [10]. We have tested the usefulness of this method for the analysis of density gradient centrifugation profiles from eukaryotic DNA, which are normally composed from overlapping Gaussian distributed profiles of several subcomponents with different mean buoyant densities. From the analysis of human DNA and from model calculations we conclude that major subcomponents can be identified by this method, if they differ in their buoyant density by approximatly 0.005 g/ml. Minor components can only be identified if the total DNA has been fractionated according to buo…

Nuclear DNA fractions with grossly different base ratios in the genome of the marine sponge Geodia cydonium

2008

The DNA of the marine sponge Geodia cydonium (G.c.), a member of the phylogenetically old phylum Porifera, was characterized by density gradient centrifugation and by determining its genetic complexity by reassociation kinetics. At least five subcomponents were identified by curve-fit analyses of analytical density gradient centrifugation profiles of total G.c.-DNA. Four of these subcomponents were isolated from total G.c.-DNA by preparative density gradient centrifugation. The GC-contents of the subcomponents were determined to be 36.4%, 44.0%, 58.7%, and 66.1%, respectively. To our knowledge, such an extreme heterogeneity of DNA composition has never before been observed for any organism.…

Demonstration and partial characterization of an intermediate HBcAG (Dane particle) population.

1981

Abstract Hepatitis-B core antigen (HBcAg) was released from Dane particles previously separated from anti-HBc by repeated pelleting through sucrose gradients separated into three HBcAg populations when analysed by cesium chloride density gradient centrifugation. Heavy HBcAg particles banded at a density of 1.355 gm/ml, intermediate HBcAg particles at a density of 1.33 gm/ml, and light mediate HBcAg particles at a density of 1.30 gm/ml. Like heavy HBcAg particles, intermediate HBcAg particles contained DNA polymerase activity, but the ratio of HBcAg to DNA polymerase activity was significantly different in both populations. Intermediate HBcAg particles could not be separated from heavy HBcAg…

Steroid hormone receptors in the female urogenital tract

1994

The increasing clinical use of estrogens in the treatment of stress-incontinent women caused us to examine receptor patterns in the female bladder and urethral tissue, and to characterize possible receptors by means of Scatchard plot analysis, competition experiments and density gradient centrifugation.

Electron-deficient trifluoromethyl-substituted sub-components affect the properties of M4L4 tetrahedral cages

2017

Two supramolecular tetrahedral cages based on a new electron-deficient trifluoromethyl-substituted pyridylimine ligand are synthesised by sub-component self-assembly. Their structures are characterised by NMR und UV-Vis spectroscopy, high-resolution mass spectrometry and single crystal X-ray diffraction. The iron(II) complex shows host–guest chemistry, complex-to-complex transformations and novel electronic properties.

Ultracentrifugation studies on the native form of the first component of human complement (C1)

1976

Dracocephalum ruyschiana L. (Ogres Kangari)

1977

Ruiša pūķgalve, atradne: Ogres rajonā, Ogres Kangaros, granstkarjera A galā priežu meža klajumiņā dienvidrietumu nogāzē, kopā ar Onobrychis, Geranium sanguineum, Filipendula hexapetala, Trifolium montanum (esparsete, asinssarkanā gandrene, lielziedu vīgrieze, kalnu āboliņš). /// Northern Dragon-head, deposit: In Ogre district, Ogres Kangaros, at the eastern end of the gravel quarry in the pine forest clearing on the south-west side, together with Onobrychis, Geranium sanguineum, Filipendula hexapetala, Trifolium montanum. [Attēls no LU Muzeja kolekcijas Herbarium Latvicum (RIG II); (BOT1029_7)]

Tendencia en la utilización de antiagregantes en la Comunidad Valenciana (2000-2005)

2007

Beatriz Román Llamosí, Rocío Broseta Solaz, Joan Quiles Izquierdo y Amalia Úbeda Pascual (Amalia.Ubeda@uv.es) Los antiagregantes plaquetarios son fármacos básicos para la prevención de enfermedades isquémicas arteriales. El objetivo de este trabajo es conocer la evolución de su utilización en Atención Primaria en la Comunidad Valenciana durante el periodo 2000-2005. Métodos: Estudio descriptivo del consumo de los antiagregantes plaquetarios (código ATC: B01AC) dispensados con cargo al Sistema Nacional de Salud en la Comunidad Valenciana en el ámbito de Atención Primaria. Datos expresados en dosis diarias definidas (DDD) por 1.000 habitantes y día. Resultados: En 2005 tres fármacos represent…