Search results for "TRIF"

showing 10 items of 1419 documents

Distribution of chlorpromazine in a simplified blood influenced by various drugs

1973

The binding of chlorpromazine to erythrocytes and to albumin as influenced by other drugs was studied in a simplified blood (31.5±0.3% bovine erythrocytes, 4 g-% bovine serum albumin in 0.02 M phosphate buffer solution containing 0.15 M NaCl). the total concentration of chlorpromazine in the simplified blood was 10−4 M, the concentration of the displacing drugs was 10−3 M. After an incubation period of 3 h at 22° C the chlorpromazine concentration was determined in the albumin solution after centrifugation of the blood at 3000×g and in the aqueous phase after ultracentrifugation at 150000×g. Under control conditions 68.1±0.9% of chlorpromazine was bound to the erythrocytes, 28.5±0.9% was bo…

ErythrocytesChlorpromazineIndomethacinSuraminBenzoatesBinding CompetitiveIncubation periodCoumarinsmedicineAnimalsDistribution (pharmacology)CentrifugationThiopentalBovine serum albuminChlorpromazinePharmacologySulfonamidesBinding SitesChromatographyQuininebiologyProbenecidChemistryFatty AcidsAqueous two-phase systemAlbuminSerum Albumin BovineGeneral MedicineChlorothiazideTetracyclineAntidepressive AgentsSalicylatesAcetazolamidePhenylbutazoneSolubilityPhenytoinbiology.proteinCattleUltracentrifugeDeoxycholic AcidProtein Bindingmedicine.drugNaunyn-Schmiedeberg's Archives of Pharmacology
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Physicochemical characterization of the fifth (C5), sixth (C6), seventh (C7), eighth (C8) and ninth (C9) component of guinea pig complement.

1971

A physicochemical characterization of the purified guinea pig complement components C5 to C9 is given. For this purpose the sedimentation rate, the diffusion coefficient, the molecular weight and the isoelectric point were determined and compared with the values already known for the guinea pig and human complement system. For the determination of the physicochemical parameters gel filtration on Sephadex G-200, ultracentrifugation applying a sucrose density gradient and thin-layer isoelectric focusing were used. By comparing the values of the human and guinea pig complement a remarkable similarity is shown.

ErythrocytesDensity gradientChemical PhenomenaImmunologySize-exclusion chromatographyGuinea PigsBiologyGuinea pigHemoglobinsCentrifugation Density GradientImmunology and AllergyAnimalsHumansChromatographyIsoelectric focusingChemistry PhysicalVenomsElectric ConductivitySnakesComplement System ProteinsCatalaseComplement systemMolecular WeightIsoelectric pointSephadexImmunoglobulin GImmunologyChromatography GelUltracentrifugeIsoelectric FocusingEuropean journal of immunology
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A preliminary study on the distribution of morphine and its glucuronides in the subcompartments of blood.

1998

[Abstract ] The distribution of morphine, morphine-3-glucuronide (M3G), and morphine-6-glucuronide (M6G) in whole blood, plasma, and packed erythrocytes was studied. Parameters investigated were the hematocrit values (10, 42, 44, and 71%) and the water content of the samples. The blood-to-plasma ratio of morphine concentrations was unaffected by variations in hematocrit and water content, whereas the corresponding ratios for M3G and M6G were strongly influenced. Ratios were 0.53 to 0.65 and 0.52 to 0.62 in specimens with average hematocrit values (42 and 44%, respectively), and the ratios were 0.81 or 0.89 (hematocrit 10%) and 0.27 or 0.28 (hemalocrit 71%) in blood samples with different he…

ErythrocytesHealth Toxicology and MutagenesisMetaboliteCentrifugationHematocritToxicologyAnalytical Chemistrychemistry.chemical_compoundPharmacokineticsBlood plasmamedicineEnvironmental ChemistryHumansSolid phase extractionWhole bloodMorphine DerivativesChemical Health and SafetyChromatographymedicine.diagnostic_testMorphineRed blood cellmedicine.anatomical_structurechemistryHematocritMorphinemedicine.drugJournal of analytical toxicology
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Phenoloxidase-dependent cytotoxic mechanism in ascidian (Styela plicata) hemocytes active against erythrocytes and K562 tumor cells.

1997

The cytotoxic activity against rabbit erythrocytes (RE) and human K562 tumor cells by Styela plicata hemocytes was significantly related to the phenoloxidase (PO) which converts phenols to quinone and initiates the melanogenic pathway. The effector hemocyte population, separated in a Percoll density gradient band, enriched in a granulocyte type named "morula cells", was examined with RE in a hemocyte cytotoxic assay and plaque forming cell assay. Inhibition experiments with the copper chelating agents 1-phenyl-2-thiourea and tropolone, the substrate analogue sodium benzoate and sodium ascorbate support the notion that hemocyte cytotoxic activity is a PO-dependent mechanism. Treatments of he…

ErythrocytesHemocytesMonophenol MonooxygenaseCytotoxicitySettore BIO/05 - ZoologiaHemocyteHydrogen PeroxideTunicateCell FractionationNitric OxidePhenylthioureaTropoloneErythrocytePhenoloxidaseCentrifugation Density GradientTumor Cells CulturedAnimalsHumansQuinoneRabbitsUrochordataK562Settore BIO/06 - Anatomia Comparata E CitologiaReactive Oxygen SpeciesEuropean journal of cell biology
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Refractive Indices and Deviations in Refractive Indices for Binary Mixtures of 1-Ethyl-3-methylimidazolium Trifluoromethanesulfonate with Methanol, E…

2009

Refractive indices of the binary mixtures of 1-ethyl-3-methylimidazolium trifluoromethanesulfonate ([emim][triflate]) with methanol, ethanol, 1-propanol, and 2-propanol were experimentally measured over the whole range of compositions at T = (288.15 to 338.15) K and atmospheric pressure. From the experimental data, deviations in refractive indices have been calculated and fitted to an extended version of the Redlich−Kister equation, which takes into account the dependence on composition and temperature simultaneously. This dependence has been discussed. The deviations are always positive, and their extent follows the sequence methanol > ethanol > 2-propanol > 1-propanol, increasing when tem…

EthanolAtmospheric pressureGeneral Chemical EngineeringAnalytical chemistryGeneral ChemistryPropanolchemistry.chemical_compound1-PropanolchemistryIonic liquidOrganic chemistryMethanolTrifluoromethanesulfonateRefractive indexJournal of Chemical & Engineering Data
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Isobaric Vapor−Liquid Equilibria for Ethyl Acetate + Ethanol + 1-Ethyl-3-methylimidazolium Trifluoromethanesulfonate at 100 kPa

2007

Isobaric vapor−liquid equilibria for the binary systems ethyl acetate + ethanol, ethyl acetate + 1-ethyl-3-methylimidazolium trifluoromethanesulfonate ([emim][triflate]), and ethanol + [emim][triflate] as well as the vapor−liquid equilibria for the ethyl acetate + ethanol + [emim][triflate] ternary system have been obtained at 100 kPa using a recirculating still. NRTL fitting parameters for the ethyl acetate + ethanol and ethanol + [emim][triflate] systems were calculated. The measured ternary data were correlated using the Mock electrolyte NRTL model, which reproduces reasonably well the experimental values. The results suggest that the addition of [emim][triflate] to the ethyl acetate + e…

EthanolTernary numeral systemGeneral Chemical EngineeringEthyl acetateGeneral ChemistryMole fractionchemistry.chemical_compoundchemistryAzeotropeIonic liquidNon-random two-liquid modelOrganic chemistryTrifluoromethanesulfonateNuclear chemistryJournal of Chemical & Engineering Data
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ChemInform Abstract: Organocatalytic Enantioselective Friedel-Crafts Alkylation of 1-Naphthol Derivatives and Activated Phenols with Ethyl Trifluorop…

2016

Financial support from the MINECO [Gobierno de Espana and FEDER (EU)] (CTQ2013-47949-P) and from Generalitat Valenciana (ISIC2012/001) is gratefully acknowledged. M.M.-M. thanks the Universitat de Valencia for a predoctoral grant. C. V. thanks MINECO for JdC contract. Access to NMR and MS facilities from the Servei central de support a la investigacio experimental (SCSIE)-UV is also acknowledged.

Ethyl trifluoropyruvatechemistry.chemical_compoundchemistrybiology1-NaphtholOrganocatalysisEnantioselective synthesisOrganic chemistryGeneral MedicinePhenolsbiology.organism_classificationValenciaFriedel–Crafts reactionChemInform
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In vivo survival rate of rabbit morulae after vitrification in a medium without serum protein.

2000

The in vivo survival rate of rabbit morulae after vitrification in a mixture of dimethyl sulphoxide and ethylene glycol solution without protein supplement (WPS) was compared with two types of protein supplements: rabbit serum (RS) and bovine serum albumin (BSA). Significant dif- ferences were observed in the percentage of transferable embryos (undamaged embryos after devit- rification, 80.4 % versus 93.2 and 92.1 %, WPS, BSA and RS, respectively, P < 0.05) and live born rate (40.9 % versus 56.1 %, WPS and BSA, respectively, P < 0.05). Non-significant differences were, however, observed in the percentages of implanted embryos at 12 days post-ovulation induc- tion (56.7, 69.7 and 68.6 %), po…

Ethylene GlycolMorulaAndrologychemistry.chemical_compoundCryoprotective AgentsPregnancyIn vivo[SDV.BDD] Life Sciences [q-bio]/Development BiologyAnimalsDimethyl SulfoxideVitrificationEmbryo ImplantationBovine serum albuminFetal DeathSurvival rate[SDV.BDLR] Life Sciences [q-bio]/Reproductive BiologyCryopreservationLagomorphabiologySerum Albumin BovineEmbryo cultureBlood ProteinsEmbryo Transferbiology.organism_classificationEmbryo transfer[SDV.AEN] Life Sciences [q-bio]/Food and NutritionBloodchemistryBiochemistrybiology.proteinFemaleRabbitsEthylene glycol
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Reactions at Interfaces: Oxygenation of n-Butyl Ligands Anchored on Silica Surfaces with Methyl(trifluoromethyl)dioxirane

2011

The oxygenation of n-butyl and n-butoxy chains bonded to silica with methyl(trifluoromethyl)dioxirane (1) revealed the ability of the silica matrix to release electron density toward the reacting C(2)-H σ-bond through the Si-C(1) and Si-O(1) σ-bonds connecting the alkyl chain to the surface (silicon β-effect). The silica surface impedes neither the alkyl chain adopting the conformation required for the silicon β-effect nor dioxirane 1 approaching the reactive C(2) methylene group. Reaction regioselectivity is insensitive to changes in the solvation of the reacting system, the location of organic ligands on the silica surface, and the H-bonding character of the silica surface. Reaction rates…

Ethylene OxideTrimethylsilylSiliconSurface PropertiesMolecular Conformationchemistry.chemical_elementChemistry Techniques SyntheticPhotochemistryReaction ratechemistry.chemical_compoundDioxiraneMethyleneAlkylchemistry.chemical_classificationTrifluoromethylOrganic ChemistryRegioselectivityEstersHydrogen BondingStereoisomerismSilicon DioxideOxygenSolutionsKineticschemistryButanesOxidation-ReductionThe Journal of Organic Chemistry
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Analysis of DNA single-strand breaks in human venous blood: a technique which does not require isolation of white blood cells.

1997

For DNA strand break analysis in human white blood cells, usually metrizoate-Ficoll centrifugation is used to isolate mononuclear cells. This procedure is time-consuming and requires at least 20 ml of blood per sample. Therefore, we developed a technique which does not require isolation of white blood cells prior to DNA strand break analysis by alkaline elution (direct method). The sensitivity of this new technique was compared to that of the standard method, which includes isolation of mononuclear blood cells. A statistically significant increase in sensitivity was observed using the direct method. After in vitro gamma-irradiation of venous blood, an increase in the elusion rate of 7.7 × 1…

Ethylene OxideVincristineEpidemiologyHealth Toxicology and Mutagenesismedicine.medical_treatmentDNA Single-StrandedMutagenBiologymedicine.disease_causePeripheral blood mononuclear cellVeinsNeoplasmsmedicineHumansCentrifugationGenetics (clinical)ChemotherapyHeparinReproducibility of ResultsCombination chemotherapyVenous bloodMolecular biologyBloodGenetic TechniquesGamma RaysToxicityImmunologymedicine.drugDNA DamageEnvironmental and molecular mutagenesis
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