Search results for "TRIX"

The generalized H-point standard-additions method to determine analytes present in two different chemical forms in unknown matrix samples. Part I. Ge…

2000

This paper shows how the generalized H-point standard-additions method (GHPSAM) can be used to obtain the total concentration or concentrations of different chemical forms of an analyte when the matrix of the sample is completely unknown. The spectral regions where the interferent behaviour can be considered as linear are found and the analyte concentration free from bias error is estimated. This paper includes the already published features of the GHPSAM and a new modification of this method which allows the simultaneous determination of two chemical forms of an analyte in a sample.

2018

We show that the measured intrinsic octupole moments of $^{220}\mathrm{Rn}$, $^{224}\mathrm{Ra}$, and $^{226}\mathrm{Ra}$ constrain the intrinsic Schiff moments of $^{225}\mathrm{Ra}$, $^{221}\mathrm{Rn}$, $^{223}\mathrm{Rn}$, $^{223}\mathrm{Fr}$, $^{225}\mathrm{Ra}$, and $^{229}\mathrm{Pa}$. The result is a dramatically reduced uncertainty in intrinsic Schiff moments. Direct measurements of octupole moments in odd nuclei will reduce the uncertainty even more. The only significant source of nuclear-physics error in the laboratory Schiff moments will then be the intrinsic matrix elements of the time-reversal noninvariant interaction produced by $CP$-violating fundamental physics. Those matri…

Micromorphological approach to the systematics of Mediterranean Isoëtes species (Isoëtaceae, Lycopodiophyta): analysis of the megaspore surface

2012

Megaspore ornamentation is one of the few morphological characters used in the taxonomy of the genus Isoëtes. In the present work, we test the application of this character for distinguishing some Isoëtes species occurring in the Mediterranean: Isoëtes sicula, which according to some authors should be included as a variety in I. histrix, and the recently described I. todaroana, whose affinity with other taxa is currently unknown. Two additional species (I. duriei, I. velata) were also included in the analysis. The megaspores were studied using scanning electron microscopy combined with energy-dispersive X-ray spectroscopy and chemical treatment. In all species, the megaspore surface is cove…

Quantitation of antigen-reactive T cells in peripheral blood by IFNgamma-ELISPOT assay and chromium-release assay: a four-centre comparative trial

2000

The ELISPOT assay is increasingly being used for the monitoring of the induction of antigen-reactive T cells in cancer vaccination trials. In order to evaluate the reliability of T cell frequency analysis with the ELISPOT assay, a comparative study was performed in four European laboratories. Six samples from healthy subjects were analyzed for the frequency of influenza-reactive CD8+ T cells in peripheral blood mononuclear cells (PBMC) by IFNgamma-ELISPOT assay. In addition, one laboratory determined cytotoxic T cell precursor (CTL) frequencies in these samples by limiting dilution chromium-release assay (LDA), and three laboratories performed a variant of the LDA, the multiple microculture…

Identification of T cell epitopes by the use of rapidly generated mRNA fragments.

2004

Abstract Although the number of defined T cell epitopes of clinically relevant antigens is constantly increasing, there is still an enormous need to identify further peptides, processed from new antigens or presented by rare HLA molecules, respectively. Here we introduce a novel two-step approach for the rapid identification of T cell epitopes. It was established in the CMV infection model. From the peripheral blood of healthy donors sharing HLA-A1 according to HLA serotyping we isolated CD8 + T lymphocytes and generated dendritic cells (DCs). DCs were electroporated with CMV pp65 mRNA and tested for recognition by autologous CD8 + T lymphocytes in IFN-γ ELISPOT assays. In all 10 CMV-seropo…

The use of clonal mRNA as an antigenic format for the detection of antigen-specific T lymphocytes in IFN-gamma ELISPOT assays.

2003

Abstract Most assay systems for the quantification of antigen-specific T-cell responses in infectious, malignant and autoimmune disease depend on the peptide antigen format and are therefore restricted to known epitopes and their presenting HLA molecules. Here we tested in ELISPOT assays the application of in vitro-transcribed clonal mRNA as an alternative antigen format covering all potential epitopes of a given antigen. As model antigens, we chose pp65 of human cytomegalovirus (HCMV) and human tyrosinase (hTyr). Antigen-presenting cells (APC) were K562 cells stably transfected with single HLA class I alleles and autologous dendritic cells (DC). As effectors, we applied in vitro-generated …

A New Approach to the Modeling of Anisotropic Media with the Transmission Line Matrix Method

2021

A reformulation of the Transmission Line Matrix (TLM) method is presented to model non-dispersive anisotropic media. Two TLM-based solutions to solve this problem can already be found in the literature, each one with an interesting feature. One can be considered a more conceptual approach, close to the TLM fundamentals, which identifies each TLM in Maxwell’s equations with a specific line. But this simplicity is achieved at the expense of an increase in the memory storage requirements of a general situation. The second existing solution is a more powerful and general formulation that avoids this increase in memory storage. However, it is based on signal processing techniques and considerabl…

A Transition Zone Complex Regulates Mammalian Ciliogenesis and Ciliary Membrane Composition

2011

Mutations in genes encoding ciliary components cause ciliopathies, but how many of these mutations disrupt ciliary function is unclear. We investigated Tectonic1 (Tctn1), a regulator of mouse Hedgehog signaling, and found that it is essential for ciliogenesis in some, but not all, tissues. Cell types that do not require Tctn1 for ciliogenesis require it to localize select membrane-associated proteins to the cilium, including Arl13b, AC3, Smoothened and Pkd2. Tctn1 forms a complex with multiple ciliopathy proteins associated with Meckel (MKS) and Joubert (JBTS) syndromes, including Mks1, Tmem216, Tmem67, Cep290, B9d1, Tctn2, and Cc2d2a. Components of the Tectonic ciliopathy complex colocaliz…

Type-II transmembrane prolyl dipeptidases and matrix metalloproteinases in membrane vesicles of active endothelial cells.

2006

Conclusions: Endothelia cells in sparse culture are migratory and increase the production of gelatinases of serine- and metallo-classes in membrane vesicles. Collectively, proteases associated with membrane vesicles degrade extracellular matrix components including type-I and type-IV collagens, laminin and fibronectin. Inhibitor studies suggest the existence of small gelatinases that were derived from these serine- and metallo-proteases. Thus, further studies are warranted to demonstrate the cooperative action of metallo- and serine proteases on cell surfaces and in extracellular vesicles during endothelial cell migration in 3D collagenous matrices, and potential proteolytic activation mech…

Robust fault detection design for unknown inputs Takagi-Sugeno models with parametric uncertainties and time-varying delays

2014

This paper deals with the design of robust fault detection system for Takagi-Sugeno (T-S) modes with parametric uncertainties and time-varying delay. An Unknown Input Observer (UIO) is designed such that the unknown inputs are thoroughly decoupled from residual signals while they show the maximum possible sensitivity to the faults and the minimum possible sensitivity to the external disturbances. Since the system under consideration is subjected to parametric uncertainties, the H ∞ model matching approach is used to design an optimal observer. Design procedure is given in terms of Linear Matrix Inequalities (LMIs). Finally, a numerical example is presented to show the effectiveness of the p…