Search results for "TUBERCULOSIS"
showing 10 items of 303 documents
Mycobacterium tuberculosis secretory proteins downregulate T cell activation by interfering with proximal and downstream T cell signalling events
2015
Background Mycobacterium tuberculosis (M. tuberculosis) modulates host immune response, mainly T cell responses for its own survival leading to disease or latent infection. The molecules and mechanisms utilized to accomplish immune subversion by M. tuberculosis are not fully understood. Understanding the molecular mechanism of T cell response to M. tuberculosis is important for development of efficacious vaccine against TB. Methods Here, we investigated effect of M. tuberculosis antigens Ag85A and ESAT-6 on T cell signalling events in CD3/CD28 induced Peripheral blood mononuclear cells (PBMCs) of PPD+ve healthy individuals and pulmonary TB patients. We studied CD3 induced intracellular calc…
Granzyme A is an additional marker for tuberculosis (TB) to discriminate between patients with active disease and subjects with latent infection.
2013
A helicopter perspective on TB biomarkers: pathway and process based analysis of gene expression data provides new insight into TB pathogenesis.
2013
Biomarker host genetic signatures are considered key tools for improved early diagnosis of tuberculosis (TB) disease (development). The analysis of gene expression changes based on a limited number of genes or single study designs, however, may not be sufficient for the identification of universal diagnostic biomarker profiles. Here we propose that biological pathway and process based analyses from multiple data sets may be more relevant for identification of key pathways in TB pathogenesis, and may reveal novel candidate diagnostic TB biomarkers. A number of independent genome-wide gene expression studies have recently been performed to study expression of biomarkers for TB disease. We hav…
IL-4 depletion enhances host resistance and passive IgA protection against tuberculosis infection in BALB/c mice.
2007
The influence of Th2 cytokines in tuberculosis has been a matter of dispute. Here we report that IL-4 has a profound regulatory effect on the infection of BALB/c mice with Mycobacterium tuberculosis. Depletion of IL-4 with a neutralizing mAb caused only evanescent reduction of lung infection, but when combined with i.n. inoculations of IgA anti-mycobacterial alpha-crystallin mAb and mouse rIFN-gamma, we observed a 40-fold reduction of the bacterial counts in the lungs at 3 wks following i.n. infection (p<0.001). In genetically deficient IL-4-/- BALB/c mice, infection in both lung and spleen was substantially reduced for up to 8 wks without further treatment. Reconstitution of IL-4-/- mice w…
Tuberculosis en el Departamento de Salud Valencia-Doctor Peset. Aportación de los métodos de microbiología molecular
2018
La tuberculosis (TB) se mantiene como problema importante de salud pública a pesar de la disminución progresiva de su incidencia en nuestro medio. Los programas de control deben incluir objetivos de mejora en detección precoz de pacientes bacilíferos y la eficacia y rapidez de los métodos de rutina microbiológica para identificación y sensibilidad de Mycobacterium tuberculosis complex, detectando cepas multirresistentes (MDR-TB) de forma rápida y sencilla. En este trabajo se describe el modelo de funcionamiento del Servicio de Microbiología en la detección y control de las TB del Departamento de Salud Valencia- Dr. Peset en los últimos once años, calculando la evolución de tasas de incidenc…
Mycobacterium tuberculosis rezistence pret antibakteriāliem preparātiem
2018
Tuberkuloze ir viens no vadošajiem nāves cēloņiem pasaulē. Par tuberkulozes izraisītāju uzskata M. tuberculosis. Praktiskajā daļā tiek veikti Zāļu jutības testi (ZJT) uz Lēvenšteina-Jensena barotnēm ar M. tuberculosis celmiem, lai noteiktu to rezistenci, kā arī tika apstrādāti rezultāti, kas bija iegūti un apkopoti SIA „RAKUS” Tuberkulozes un plaušu slimību centra Mikobakterioloģijas laboratorijas informācijas sistēmā(LIS). Statistikas analīzē tiek pierādīts, ka pasaulē un Latvijā saslimstības ar tuberkulozes gadījumu skaits samazinās un, savukārt, izārstēto cilvēku skaits paaugstinās. Starp visiem rezistentiem gadījumiem visbiežāk rezistence bija pret pirazinamīdu un izoniazīdu no pirmās r…
Hydroquinone derivatives from the marine-derived fungus Gliomastix sp.
2017
Eight new hydroquinone derivatives, gliomastins A–D (1–4), 9-O-methylgliomastin C (5), acremonin A 1-O-β-D-glucopyranoside (6), gliomastin E 1-O-β-D-glucopyranoside (7), and 6′-O-acetyl-isohomoarbutin (8), together with seven known analogues were isolated from the marine-derived fungus Gliomastix sp. Their structures were elucidated by extensive spectroscopic analysis including 1D and 2D NMR measurements aided by DFT NMR calculations as well as MS data. TDDFT-ECD and OR calculations were performed to determine the absolute configurations of 1 and the aglycones of 6 and 7. Compound 1 features a novel skeleton, biogenetically derived from a Diels–Alder reaction between derivatives of 11 and 1…
BLAST Database with all accesible non-tuberculous mycobacteria genomic sequences (Oct, 2018)
2019
In our work "Towards next generation diagnostics for tuberculosis: identification of novel molecular targets by large-scale comparative genomics" (https://doi.org/10.1101/569384 ). We look for M. tuberculosis genes that are not present in organisms other tan MTBC. This database corresponds with the described database of 4,277 NTM assemblies.
Diabetes in active tuberculosis in low-income countries: to test or to take care?
2019
Characterization of HLA-DR- and TCR-binding residues of an immunodominant and genetically permissive peptide of the 16-kDa protein of Mycobacterium t…
2004
The 16-kDa protein of Mycobacterium tuberculosis represents an important antigenic target during bacillary latency and, consequently, should be considered as candidate subunit vaccine component. In this study, we have used CD4 T cell clones that recognize the peptide p91-110, an immunodominant and genetically permissive epitope, in the context of five different HLA-DR molecules and truncated and substituted variants of this peptide, to identify the minimal binding sequence (HLA-DR-binding core) and the minimal stimulatory sequence (TCR-binding core), as well as the residues that contact HLA-DR molecules and the TCR. We have found a common 9-mer sequence, spanning amino acids 93-101, as the …