Search results for "Techniques"

Dipeptidyl Enoates As Potent Rhodesain Inhibitors That Display a Dual Mode of Action

2015

Dipeptidyl enoates were prepared through a high-yielding two-step synthetic route. They have a dipeptidic structure with a 4-oxoenoate moiety as a warhead with multiple reactive sites. Dipeptidyl enoates were screened against rhodesain and human cathepsins B and L, and were found to be potent and selective inhibitors of rhodesain. Among them (S,E)-ethyl 5-((S)-2-{[(benzyloxy)carbonyl]amino}-3-phenylpropanamido)-7-methyl-4-oxooct-2-enoate (6) was the most potent, with an IC50 value of 16.4 nm and kinact/Ki=1.6×106 m−1 s−1 against rhodesain. These dipeptidyl enoates display a reversible mode of inhibition at very low concentrations and an irreversible mode at higher concentrations. Inhibition…

Extending Physics Potential of Large Liquid Scintillator Neutrino Detectors

2016

La "salle du moulin" à la forge de l'abbaye de Fontenay

1996

P. Benoit et L. Pressouyre; International audience

Female reproductive factors are associated with objectively measured physical activity in middle-aged women

2017

Physical activity improves health and may delay the onset of several chronic diseases. For women in particular, the rate of these diseases accelerates at middle age; therefore it is important to identify the determinants of health-enhancing physical activity during midlife in this population. In this study, we focused on determinants that are unique to the female sex, such as childbearing and menopause. The main objective was to characterize the level of physical activity and differences between active and inactive middle-aged Finnish women. In addition, we examined the association of physical activity with female reproductive factors at midlife. The study population consisted of 647 women …

Variational approach for analysis of harmonic vibration and stabiligy of moving panels

2014

In this paper, the stability of a simply supported axially moving elastic panel (plate undergoing cylindrical deformation) is considered. A complex variable technique and bifurcation theory are applied. As a result, variational equations and a variational principle are derived. Analysis of the variational principle allows the study of qualitative properties of the bifurcation points. Asymptotic behaviour in a small neighbourhood around an arbitrary bifurcation point is analyzed and presented. It is shown analytically that the eigenvalue curves in the (ω, V0) plane cross both the ω and V0 axes perpendicularly. It is also shown that near each bifurcation point, the dependence ω(V0) for each m…

« Deirdre O’Mahony, artiste et archéologue des savoirs techniques indigènes »

2022

Les projets participatifs et collaboratifs de l’artiste irlandaise Deirdre O’Mahony, ancrés dans le monde rural du Burren, valorisent le patrimoine vernaculaire et les savoir-faire indigènes. Ses recherches de terrain portant sur l’évolution du paysage abordé comme milieu et les collaborations multiples qu’elle entreprend aboutissent à des propositions artistiques revisitant le land art. Ses interventions dans le paysage sont souvent complétées par des propositions dialogiques, dont le repas partagé, qui invite les participants à explorer l’histoire des lieux, les jeux de pouvoir qui s’y tissent et la pertinence des savoirs vernaculaires pour les enjeux de demain, notamment en matière de sé…

CVB3 VP1 interacts with MAT1 to inhibit cell proliferation by interfering with Cdk-activating kinase complex activity in CVB3-induced acute pancreati…

2021

Coxsackievirus B3 (CVB3) belongs to the genus Enterovirus of the family Picornaviridae and can cause acute acinar pancreatitis in adults. However, the molecular mechanisms of pathogenesis underlying CVB3-induced acute pancreatitis have remained unclear. In this study, we discovered that CVB3 capsid protein VP1 inhibited pancreatic cell proliferation and exerted strong cytopathic effects on HPAC cells. Through yeast two-hybrid, co-immunoprecipitation, and confocal microscopy, we show that Menage a trois 1 (MAT1), a subunit of the Cdk-Activating Kinase (CAK) complex involved in cell proliferation and transcription, is a novel interaction protein with CVB3 VP1. Moreover, CVB3 VP1 inhibited MAT…

Internalization of novel non-viral vector TAT-streptavidin into human cells

2007

BMC Biotechnology, 7 (1)

Baculovirus display strategies: Emerging tools for eukaryotic libraries and gene delivery

2004

Recombinant baculoviruses have been extensively used as vectors for abundant expression of a large variety of foreign proteins in insect cell cultures. The appeal of the system lies essentially in easy cloning techniques and virus propagation combined with the eukaryotic post-translational modification machinery of the insect cell. Recently, a novel molecular biology tool was established by the development of baculovirus surface display, using different strategies for presentation of foreign peptides and proteins on the surface of budded virions. This eukaryotic display system enables presentation of large complex proteins on the surface of baculovirus particles and has thereby become a ver…

The Nasal Epithelium as a Factory for Systemic Protein Delivery

2002

We have previously shown that recombinant Sendai virus (SeV) produces efficient in vivo airway epithelial gene transfer. The ability to produce therapeutic levels of circulating proteins following noninvasive gene transfer would have widespread clinical application. Here, we compared nose, lung, and skeletal muscle for the ability to produce circulating levels of the secreted mouse antiinflammatory cytokine interleukin-10 (IL10) following SeV-mediated gene transfer. High levels of serum IL10 were obtained from each site with a potency order of lung > nose > muscle for a given viral titer. Serum levels from each site were within the likely required range for anti-inflammatory effects. The co…