Search results for "Template"

Novel simple templates for reproducible positioning of skin applicators in brachytherapy.

2016

Purpose : Esteya and Valencia surface applicators are designed to treat skin tumors using brachytherapy. In clinical practice, in order to avoid errors that may affect the treatment outcome, there are two issues that need to be carefully addressed. First, the selected applicator for the treatment should provide adequate margin for the target, and second, the applicator has to be precisely positioned before each treatment fraction. In this work, we describe the development and use of a new acrylic templates named Template La Fe-ITIC. They have been designed specifically to help the clinical user in the selection of the correct applicator, and to assist the medical staff in reproducing the po…

Automatic Acoustic Target Detecting and Tracking on the Azimuth Recording Diagram with Image Processing Methods

2019

International audience; Passive acoustic target detection has been a hot research topic for a few decades. Azimuth recording diagram is one of the most promising techniques to estimate the arrival direction of the interested signal by visualizing the sound wave information. However, this method is challenged by the random ambient noise, resulting in low reliability and short effective distance. This paper presents a real-time postprocessing framework for passive acoustic target detection modalities by using a sonar array, in which image processing methods are used to automate the target detecting and tracking on the azimuth recording diagram. The simulation results demonstrate that the prop…

A practical method for barcoding and size-trimming PCR templates for amplicon sequencing

2016

Sample barcoding facilitates the analysis of tens or even hundreds of samples in a single next-generation sequencing (NGS) run, but more efficient methods are needed for high-throughput barcoding and size-trimming of long PCR products. Here we present a two-step PCR approach for barcoding followed by pool shearing, adapter ligation, and 5′ end selection for trimming sets of DNA templates of any size. Our new trimming method offers clear benefits for phylogenetic studies, since targeting exactly the same region maximizes the alignment and enables the use of operational taxonomic unit (OTU)-based algorithms.

Manganese Ions Individually Alter the Reverse Transcription Signature of Modified Ribonucleosides

2020

Reverse transcription of RNA templates containing modified ribonucleosides transfers modification-related information as misincorporations, arrest or nucleotide skipping events to the newly synthesized cDNA strand. The frequency and proportion of these events, merged from all sequenced cDNAs, yield a so-called RT signature, characteristic for the respective RNA modification and reverse transcriptase (RT). While known for DNA polymerases in so-called error-prone PCR, testing of four different RTs by replacing Mg2+ with Mn2+ in reaction buffer revealed the immense influence of manganese chloride on derived RT signatures, with arrest rates on m1A positions dropping from 82% down to 24%. Additi…

Rational Design of Thermoresponsive Microgel Templates with Polydopamine Surface Coating for Microtissue Applications.

2021

Functional microgels provide a versatile basis for synthetic in vitro platforms as alternatives to animal experiments. The tuning of the physical, chemical, and biological properties of synthetic microgels can be achieved by blending suitable polymers and formulating them such to reflect the heterogenous and complex nature of biological tissues. Based on this premise, this paper introduces the development of volume-switchable core-shell microgels as 3D templates to enable cell growth for microtissue applications, using a systematic approach to tune the microgel properties based on a deep conceptual and practical understanding. Microscopic microgel design, such as the tailoring of the microg…

Reflections about the authentic document in "post mortem" fecundation

2018

[EN] The legislation on assisted reproduction contemplates the ¿post mortem¿ fecundation. Nevertheless, the document for the presentation of the assent raises some doubts in relation with the principle of party autonomy and the new technologies of the information and communication (ICTs). In the present work one thinks about these questions and one tries to contribute some solution of application in the Spanish legal system.

Generics: Ada 95 vs. C++ vs. Java 1.5

2005

Ni-Based Alloy Nanostructured Electrodes for Alkaline Electrolyzers

In water alkaline electrolyzer field, the development of Nickel-based nanostructured electrode is one of the possible ways to improve the water electrolysis efficiency

Ruthenium Oxide Nanotubes Via Template Electrosynthesis

2011

Ruthenium oxide nanotubes were fabricated by a single-step galvanostatic deposition using porous anodic alumina membrane as template. For the electrodeposition process, we used a electrochemical cell specifically designed in order to employ only 0.5 ml of 0.02 M RuCl3•xH2O solution. The deposition from a very small volume was specifically addressed owing to the high cost of ruthenium compounds, which could be of some relevance from an applicative point of view. Several techniques were used to characterize the samples prior to and after thermal treatment, which was carried out at different temperatures in order to study the crystallization process of the deposit. Raman spectroscopy of as-dep…

Tin(IV) catalyzed D-galacturonic acid anomerization

2008

A comparative kinetic study of anomerization between the four main forms (alpha/beta-pyranosides and alpha/beta-furanosides) of D-galacturonic acid (GalAH(2), 1) and tin(IV) complexed D-galacturonate ([Sn(CH(3))(2)(GalA)(H(2)O)(2)], 2) in D(2)O, is reported. Important differences are shown by pD vs. mutarotation rate profiles of 1 and 2. Coordination, in fact, causes: i) a spectacular increase of the rate due to kinetic template assistance of the carboxylic tail holding the metal in close proximity to the reactive centre and ii) a change in the dependence of the rate on the pD. Entropic and enthalpic factors contribute to the huge activation energy decrease in 2. Anomerization rates of the …