Search results for "Testosterone"
showing 10 items of 420 documents
Constitutive and inducible expression of CYP enzymes in immortal hepatocytes derived from SV40 transgenic mice
2003
1. The expression of liver-specific transcription factors and cytochrome P450 (CYP) enzymes have been studied in three new hepatocyte-like cell lines derived from SV Delta 202 transgenic mice: AMH-Delta 202 (adult mouse hepatocytes), TAMH-Delta 202 (tumour-derived adult mouse hepatocytes) and NMH-Delta 202 (newborn mouse hepatocytes). 2. mRNA levels of liver-enriched transcription factors such as D-element binding protein (DBP), liver-enriched transcription activating protein (LAP) and the hepatic nuclear factors (HNF) 1, 2 and 3 in all Delta 202 transgenic hepatocyte lines were similar to those in the wild-type liver and in primary mouse hepatocytes. 3. Analysis of basal CYP activities and…
Salivary Testosterone and Cortisol as Biomarkers for the Diagnosis of Sarcopenia and Sarcopenic Obesity in Community-Dwelling Older Adults
2021
Nowadays, the appearance of sarcopenia (S) or sarcopenic obesity (SO) is related to aging. According to the criteria of the European Working Group on Sarcopenia in Older People (EWGSOP), the feasibility of using salivary cortisol and testosterone levels was analyzed as diagnostic biomarkers of S or SO. One hundred and ninety non-institutionalized people aged &ge
Effects of high-intensity training on physiological and hormonal adaptions in well-trained cyclists
2017
Purpose: This study aimed to compare the effects of three different high-intensity training (HIT) models, balanced for total load but differing in training plan progression, on endurance adaptations. Methods: Sixty-three cyclists (peak oxygen uptake (V˙ O2peak) 61.3 T 5.8 mLIkgj1 Iminj1 ) were randomized to three training groups and instructed to follow a 12-wk training program consisting of 24 interval sessions, a high volume of low-intensity training, and laboratory testing. The increasing HIT group (n = 23) performed interval training as 4 16 min in weeks 1–4, 4 8 min in weeks 5–8, and 4 4 min in weeks 9–12. The decreasing HIT group (n = 20) performed interval sessions in the opposite me…
IOC consensus statement on relative energy deficiency in sport (RED-S): 2018 update
2018
In 2014, the IOC published a consensus statement entitled ‘Beyond the Female Athlete Triad: Relative Energy Deficiency in Sport (RED-S)’. The syndrome of RED-S refers to ‘impaired physiological functioning caused by relative energy deficiency and includes, but is not limited to, impairments of metabolic rate, menstrual function, bone health, immunity, protein synthesis and cardiovascular health’. The aetiological factor of this syndrome is low energy availability (LEA).1 The publication of the RED-S consensus statement stimulated activity in the field of Female Athlete Triad science, including some initial controversy2 3 followed by numerous scientific publications addressing: 1. The health…
New Molecular Targets and Lifestyle Interventions to Delay Aging Sarcopenia
2014
The term sarcopenia was originally created to refer age-related loss of muscle mass with consequent loss of strength (Morley et al., 2001). There are now four international definitions of sarcopenia (Cruz-Jentoft et al., 2010; Muscaritoli et al., 2010; Morley et al., 2011). In essence they all agree, requiring a measure of walking capability [either low gait speed or a limited endurance (distance) in a 6-min walk], together with an appendicular lean mass of <2 SDs of a sex and ethnically corrected normal level for individuals 20–30 years old. Sarcopenia is a prevalent health problem among the elderly. On average, 5–13 and 11–50% of people aged 60−70 years and ≥80 years, respectively suffer …
Gender- and season-dependent relationships between testosterone, oestradiol and immune functions in wild roach
2004
Plasma testosterone and 17β-oestradiol concentrations, differential leukocyte counts and proportion of dead Rhipidocotyle campanula gill parasites (parasite resistance) were determined five times during a year in two populations of roach Rutilus rutilus and analysed for seasonal and gender differences. In addition to the above immune variables, plasma Immunoglobulin M (IgM) concentration, chemiluminescence and migration differential of head kidney phagocytes, size of the spleen, haematocrit and total leukocyte count were correlated with sex hormones for each population, sampling time and sex separately, using condition factor as a partial correlate. There were no clear gender differences in…
Gonadotropic Therapy in Men with Hypogonadotropic Hypogonadism
1984
Many therapeutic regimens, differing in dose and duration, have been employed in the treatment of hypogonadotropic hypogonadism. This study presents the results from a regimen of hCG plus hMG plus testosterone combined treatment in 15 patients affected by selective hypogonadotropic hypogonadism. The patients were homogeneous in age (16–23 years), testicular size, aspermia, absence of prior hormonal treatment, and normal karyotype. They were administered 5000 IU of hCG plus 75 IU of hMG twice a week for two months and a single dose of depo-testosterone (250 mg) in the third month. This therapeutic plan was carried out continuously for eight three-month cycles (24 months). Sexual maturation a…
Ausgeprägte Virilisierung in der Postmenopause - eine Falldarstellung
2005
Severe postmenopausal virilization is a rare event in clinical practice. To evaluate ovarian or adrenal hyperandrogenism endocrine tests and imaging are useful diagnostic tools. We report a case of a postmenopausal woman with hirsutism and androgenetic alopecia. A malignant cause for the present disorder could be excluded by imaging. Selective venous sampling was administered with increased testosterone level of the right adrenal vein. Right adrenalectomy and right oophorctomy was recommended.
Androgen hydroxylation catalysed by a cell line (SD1) that stably expresses rat hepatic cytochrome P-450 PB-4 (IIB1).
1989
Androgen hydroxylation catalysed by Chinese hamster fibroblast SD1 cells, which stably express cytochrome P-450 form PB-4, the rat P450IIB1 gene product, was assessed and compared to that catalysed by purified cytochrome P-450 PB-4 isolated from rat liver. SD1 cell homogenates catalysed the NADPH-dependent hydroxylation of androstenedione and testosterone with a regioselectivity very similar to that purified by P-450 PB-4 (16 beta-hydroxylation/16 alpha-hydroxylation = 6.0-6.8 for androstenedione; 16 beta/16 alpha = 0.9 for testosterone). Homogenates prepared from the parental cell line V79, which does not express detectable levels of P-450 PB-4 or any other cytochrome P-450, exhibited no a…
Síndrome de déficit de testosterona y disfunción eréctil
2010
OBJETIVOS Analizar la relacion entre el sindrome de deficit de testosterona (SDT) y la disfuncion erectil y sus implicaciones diagnosticas y terapeuticas. METODOS Revision bibliografica mediante la base de datos de Pubmed de la US National Library of Medicine. RESULTADOS La real prevalencia del SDT es desconocida, debido a la falta de criterios diagnosticos uniformes en cuanto a que fraccion de testosterona debe medirse (total, libre o biodisponible) y cuales deben ser los valores diagnosticos. A pesar de ello, se estima que entre un 5-15% de varones con disfuncion erectil presentan niveles sericos disminuidos de testosterona. Existe una solida base experimental que demuestra que la testost…