Search results for "Throughput"
showing 10 items of 483 documents
Taking digitized points quality into account in geometrical specification measurement by laser sensor
2004
International audience; Today, the new measurement techniques without contact like the laser sensors are used more and more in industrial applications. They allow to obtain many points in a short time. However these techniques have a less good accuracy than the classical process with contact probe for metrology application. The work presented in this paper, is part of a research project on the in-process product inspection. In the case of in-process product inspection, the throughput time must be the shortest with a good dimensional accuracy. So, it is necessary to increase the measurement accuracy of the points cloud for using the laser scanners. For this first approach, we suggest to buil…
Nodulated root imaging within the high throughput plant phenotyping platform (PPHD, INRA, Dijon)
2014
Présentation orale/ Communication avec actes
Exploring the diversity of listeria monocytogenes biofilm architecture by high-throughput confocal laser scanning microscopy and the predominance of …
2015
ABSTRACT Listeria monocytogenes is involved in food-borne illness with a high mortality rate. The persistence of the pathogen along the food chain can be associated with its ability to form biofilms on inert surfaces. While most of the phenotypes associated with biofilms are related to their spatial organization, most published data comparing biofilm formation by L. monocytogenes isolates are based on the quantitative crystal violet assay, which does not give access to structural information. Using a high-throughput confocal-imaging approach, the aim of this work was to decipher the structural diversity of biofilms formed by 96 L. monocytogenes strains isolated from various environments. Pr…
Assessment of Adult Mouse Brain Neuroanatomical Phenotypes Using Quantitative and Precision Histology
2022
Modelling human neurodevelopmental disorders is important in biomedical research since the brain cannot be easily accessed in humans. In this chapter, we describe a series of standardized procedures for the reliable analysis of neuroanatomical phenotypes (NAPs) of the adult mouse brain using quantitative 2D histological practices. Our goal is to provide the reader an experimental pipeline, ranging from experimental work through to data analysis, which can be performed in any academic research setting with or without access to a histology platform. Depending on the type of sections studied, parasagittal or coronal, the assessment of brain neuroanatomy is performed at stereotaxic sections, at…
GnS-PIPE: an optimized bionformatic pipeline to efficiently assess microbial taxonomic diversity of complex environments using high throughput sequen…
2013
International audience; The rRNA genes (16S, 18S, ITS) are widely used to study microbial communities in soils, as they can be easily amplified from metagenomic DNA. Moreover, the recent development of high-throughput sequencing technologies allows the assessment of millions of sequences from a single metagenomic DNA. Some pipelines are already available (e.g. QIIME or Mothur) to efficiently treat such data. However, the development of bioinformatic tools must now be validated by various biological tests. This was particularly true for key steps to appraise microbial diversity and richness. Here, we present a new pipeline named GnS-PIPE, a software application performing bacterial, archaeal…
GnS-PIPE: an optimized bionformatic pipeline to efficiently assess microbial taxonomic diversity of complex environments using high throughput sequen…
2013
International audience; The rRNA genes (16S, 18S, ITS) are widely used to study microbial communities in soils, as they can be easily amplified from metagenomic DNA. Moreover, the recent development of high-throughput sequencing technologies allows the assessment of millions of sequences from a single metagenomic DNA. Some pipelines are already available (e.g. QIIME or Mothur) to efficiently treat such data. However, the development of bioinformatic tools must now be validated by various biological tests. This was particularly true for key steps to appraise microbial diversity and richness. Here, we present a new pipeline named GnS-PIPE, a software application performing bacterial, archaeal…
In the context of sustainable agriculture how, with plant high throughput phenotyping, can we address the various challenges?
2018
International audience; In a context of climate change and soil and water resource degradation, it becomes increasingly important to reduce the need for high nutrient, water, or pesticides inputs, leading to more sustainable agricultural practices. In this context, our aim is to select the best performing crops in various deleterious abiotic environments, having both a higher yield and a better quality, a better environmental « efficiency ». Because plants interact with numerous and diverse microorganisms, especially in the soil volume surrounding roots, called rhizosphere, plant-microorganism relationships in the rhizosphere is also of great agronomical and ecological importance. To addres…
High-throughput sequencing of amplicons for monitoring yeast biodiversity in must and during alcoholic fermentation
2014
Abstract We compared pyrosequencing technology with the PCR-ITS-RFLP analysis of yeast isolates and denaturing gradient gel electrophoresis (DGGE). These methods gave divergent findings for the yeast population. DGGE was unsuitable for the quantification of biodiversity and its use for species detection was limited by the initial abundance of each species. The isolates identified by PCR-ITS-RFLP were not fully representative of the true population. For population dynamics, high-throughput sequencing technology yielded results differing in some respects from those obtained with other approaches. This study demonstrates that 454 pyrosequencing of amplicons is more relevant than other methods …
Gas sensors arrays (electronic noses) : a study about the speed/accuracy ratio
2000
Abstract New Fingerprint Mass Spectra (FMS) systems claim for a higher precision, a reduced analysis time, and a lower drift, compared with Gas Sensors Arrays ‘Electronic Noses’. We demonstrated in this study that metal oxide gas sensors may have highly improved performances for both accuracy and run time, when used in optimum conditions. The different noise components were quantified referring to popular words (equivalent of ethanol concentration) using the transfer function of the sensor. It was pointed out that sample surrounding greatly affects the noise level, and that the signal-to-noise ratio may be dramatically increased, reducing the distortion generally assessed between sensor and…
Electronic noses : specify or disappear
2000
Abstract When the quality control is achieved by using GC or GC/MS, the apparatus must comply with the applicable norms, but what about “electronic noses”? End users demand for formal specifications for selectivity, sensitivity, repeatability and sample throughput. The behavioural modelling of electronic olfactometers may provide specifications for these required parameters. This will allow both the measure itself, and evaluate the influence of the experimental errors on the sample classification. Users may expect to find a methodology allowing the performances of systems to be checked before any final decision and during routine use. We have demonstrated that the system sensitivity and log…