Search results for "Titi"
showing 10 items of 6322 documents
Single-chain antibodies against a plant viral RNA-dependent RNA polymerase confer virus resistance.
2004
Crop loss due to viral diseases is still a major problem for agriculture today. We present a strategy to achieve virus resistance based on the expression of single-chain Fv fragments (scFvs) against a conserved domain in a plant viral RNA-dependent RNA polymerase (RdRp), a key enzyme in virus replication. The selected scFvs inhibited complementary RNA synthesis of different plant virus RdRps in vitro and virus replication in planta. Moreover, the scFvs also bound to the RdRp of the distantly related hepatitis C virus. T(1) and T(2) progeny of transgenic lines of Nicotiana benthamiana expressing different scFvs either in the cytosol or in the endoplasmic reticulum showed varying degrees of r…
Biochemical and structural analysis of the NS5B RNA-dependent RNA polymerase of the hepatitis C virus.
2000
Hepatitis C virus (HCV), the major causative agent of chronic and sporadic non-A, non-B hepatitis worldwide, is a distinct member of the Flaviviridae virus family. These viruses have in common a plus-strand RNA genome that is replicated in the cytoplasm of the infected cell via minus-strand RNA intermediates. Owing to the lack of reliable cell culture systems and convenient animal models for HCV, the mechanisms governing RNA replication are not known. As a first step towards the development of appropriate in vitro systems, we expressed the NS5B RNA-dependent RNA polymerase (RdRp) in insect cells, purified the protein to near homogeneity and studied its biochemical properties. It is a primer…
Modulation of Hepatitis C Virus NS5A Hyperphosphorylation by Nonstructural Proteins NS3, NS4A, and NS4B
1999
NS5A of the hepatitis C virus (HCV) is a highly phosphorylated protein involved in resistance against interferon and required most likely for replication of the viral genome. Phosphorylation of this protein is mediated by a cellular kinase(s) generating multiple proteins with different electrophoretic mobilities. In the case of the genotype 1b isolate HCV-J, in addition to the basal phosphorylated NS5A (designated pp56), a hyperphosphorylated form (pp58) was found on coexpression of NS4A (T. Kaneko, Y. Tanji, S. Satoh, M. Hijikata, S. Asabe, K. Kimura, and K. Shimotohno, Biochem. Biophys. Res. Commun. 205:320‐326, 1994). Using a comparative analysis of two full-length genomes of genotype 1b…
Functional properties of a monoclonal antibody inhibiting the hepatitis C virus RNA-dependent RNA polymerase.
2001
The hepatitis C virus (HCV) RNA-dependent RNA polymerase (RdRp), represented by nonstructural protein 5B (NS5B), has recently emerged as a promising target for antiviral intervention. Here, we describe the isolation, functional characterization, and molecular cloning of a monoclonal antibody (mAb) inhibiting the HCV RdRp. This mAb, designated 5B-12B7, binds with high affinity to a conformational epitope in the palm subdomain of the HCV RdRp and recognizes native NS5B expressed in the context of the entire HCV polyprotein or subgenomic replicons. Complete inhibition of RdRp activity in vitro was observed at equimolar concentrations of NS5B and mAb 5B-12B7, whereas RdRp activities of classica…
Evaluation of HBs, HBc, and frCP virus-like particles for expression of human papillomavirus 16 E7 oncoprotein epitopes.
2002
<i>Objectives:</i> In an attempt to develop virus-like particles (VLPs) as experimental vaccine against human papilloma virus (HPV)-induced tumours, the HPV16 E7 oncoprotein epitopes spanning amino acid (aa) residues 35–98 were expressed on three proteins capable of VLP formation: hepatitis B virus (HBV) surface (HBs) and core (HBc) antigens, and RNA phage fr coats (frCP). <i>Methods:</i> The profile of immunoglobulin isotypes induced in Balb/C mice after immunization with purified chimeric proteins was studied. <i>Results:</i> The HBs*-E7(35–54) protein expressing E7 residues 35–54 between residues 139 and 142 of the HBs carrier formed HBs-like particles…
Tumor targeting of baculovirus displaying a lymphatic homing peptide.
2008
Background Tumor-associated cells and vasculature express attractive molecular markers for site-specific vector targeting. To attain tumor-selective tropism, we recently developed a baculovirus vector displaying the lymphatic homing peptide LyP-1, originally identified by ex vivo/in vivo screening of phage display libraries, on the viral envelope by fusion to the transmembrane anchor of vesicular stomatitis virus G-protein. Methods In the present study, we explored the specificity and kinetics of viral binding and internalization as well as in vivo tumor homing of the LyP-1 displaying virus to elucidate the applicability of baculovirus for targeted therapies. Results We demonstrated that th…
Wybory niekonkurencyjne w Polsce na poziomie lokalnym – wstępne oceny
2020
Artykuł podejmuje problem obsadzania mandatów bez głosowania w wyborach do rad gmin w gminach liczących do 20 tys. mieszkańców. W stosunku do wyborów z 2014 r. liczba takich przypadków wzrosła w 2018 r. o 77%. Wydaje się, że obowiązujące prawo wyborcze, w szczególności art. 418 § 1 (podział gmin na jednomandatowe okręgi wyborcze), art. 434 § 1 i 2 (zasady organizacji wyborów w przypadku zgłoszenia liczby kandydatów równej liczbie mandatów do obsadzenia) i art. 478 § 2 (tryb zgłaszania kandydatów na wójta lub burmistrza) Kodeksu wyborczego, w połączeniu z uwarunkowaniami lokalnej rywalizacji wyborczej, może sprzyjać powstawaniu zjawiska niekonkurencyjnych wyborów, które skutkują m.in. spadki…
Palermo
2021
Il capitolo analizza la distribuzione del disagio sociale nelle aree suburbane della città e si concentra sulla relazione fra disagio sociale e comportamento elettorale.
Retail banking in the aftermath of the financial crisis
2018
This thesis considers the effects of the global financial crisis that started in 2008 on the retail banking environment and on banks’ behavior during and in the aftermath of the crisis. The thesis consists of an introductory chapter and three empirical articles that use unique datasets on Finnish cooperative banks. The introductory chapter provides background and an overview of the thesis and considers the effects of the crisis on retail banking from an overall perspective. The empirical articles investigate the roles of a competitive environment, a low interest rate environment, and relationship banking during the crisis and in the post-crisis period. The first article looks at whether the…
Dlaczego warto jeść piętki chleba? : o przesądach kulinarnych
2016
Z kulinariami związanych jest wiele różnorodnych przesądów. W kuchni polskiej znajduje się mnóstwo składników żywieniowych, których spożywanie na przestrzeni wieków obrosło wieloma wierzeniami. W niniejszym artykule omówiono zachowania przesądne dotyczące wybranych składników żywnościowych (chleba, kapusty, ziemniaków, cukru) oraz rekwizytów kuchennych (garnków i sztućców). Zaprezentowane materiały pochodzą z dwóch źródeł: zastanych (opracowań etnograficznych wydanych przed 1945 r.) i wywołanych (zebranych podczas wywiadów prowadzonych w 2015 r. w obrębie dwóch województw - śląskiego i opolskiego).