Search results for "Toxicity"

Cytotoxicity, biaccessibility and transport by Caco-2 cells of enniatins and beauvericin

2011

General Cytotoxicity Assessment by Means of the MTT Assay

2014

Cytotoxicity assays were among the first in vitro bioassay methods used to predict toxicity of substances to various tissues. In vitro cytotoxicity testing provides a crucial means for safety assessment and screening, and for ranking compounds. The choice of using a particular cytotoxicity assay technology may be influenced by specific research goals. As such, four main classes of assays are used to monitor the response of cultured cells after treatment with potential toxicants. These methods measure viability, cell membrane integrity, cell proliferation, and metabolic activity. In this chapter, we focus on the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tetrazolium reducti…

Evaluation of the Cytotoxicity of Cholesterol Oxides in Human Colon Cancer Caco-2 Cells

2014

The content of cholesterol oxides (COPs) in foods varies between 0.1 and 294.3 mg/g. These oxides are formed by auto-oxidative enzymatic processes promoted by the heat treatment of food and/or to exposition of them to the presence of oxygen and sunlight during storage. Their importance is that they are associated with pathological processes like apoptosis, dyslipidemia and pro-oxidative states, among others. The objective was to evaluate the cytotoxicity, by means MTT assay, of 7-keto cholesterol (7KC), cholestane-triol (Triol), α-epoxy cholesterol (α-epoxy C), β-epoxy cholesterol (β-epoxy C) in differentiated Caco-2 cells at 120μM for 24, 48 and 72h.Triol was the most cytotoxic COP, with a…

Abbau und Toxizität von Hexachlorbicyclo [2.2.1]-hept-5-en- Derivaten (Cyclodien-Insektizide) / Structural Aspects of Biological Conversion and Toxic…

1973

The aspects of molecular-structure and biological conversion have been studied for 12 derivatives of the endosulfan ether 1 [4, 5, 6, 7, 8, 8- hexachloro- 1, 3, 3a, 4, 7, 7a- hexahydro- 4, 7-methanoisobenzofuran]. Adults of a normal house-fly strain (Musca domestica) have been used in the assay. Derivatives of 1 monosubstituted in position I are found to be converted substantially faster than products disubstituted in position I and 3, respectively. Metabolism is inhibited for all compounds studied after application of piperonyl butoxide as a synergist. Besides proving that on oxidative metabolism has to be discussed and in fact could be proved for some derivatives, the intrinsic toxicity o…

Aloe-emodin's cytotoxicity against CCRF-CEM cells: NF-κB as a major player induces apoptosis

2017

Interactive effects of boron and salinity on greenhouse tomato grown in closed soilless system.

2012

Germil-un silil-heterociklu sintēze, struktūra un bioloģiskā aktivitāte

2013

Elektroniskā versija nesatur pielikumus

Behavior of Calcium Phosphate–Chitosan–Collagen Composite Coating on AISI 304 for Orthopedic Applications

2022

Calcium phosphate/chitosan/collagen composite coating on AISI 304 stainless steel was investigated. Coatings were realized by galvanic coupling that occurs without an external power supply because it begins with the coupling between two metals with different standard electrochemical potentials. The process consists of the co-deposition of the three components with the calcium phosphate crystals incorporated into the polymeric composite of chitosan and collagen. Physical-chemical characterizations of the samples were executed to evaluate morphology and chemical composition. Morphological analyses have shown that the surface of the stainless steel is covered by the deposit, which has a very r…

Safety of Dietary Guanidinoacetic Acid: A Villain of a Good Guy?

2021

Guanidinoacetic acid (GAA) is a natural amino acid derivative that is well-recognized for its central role in the biosynthesis of creatine, an essential compound involved in cellular energy metabolism. GAA (also known as glycocyamine or betacyamine) has been investigated as an energy-boosting dietary supplement in humans for more than 70 years. GAA is suggested to effectively increase low levels of tissue creatine and improve clinical features of cardiometabolic and neurological diseases, with GAA often outcompeting traditional bioenergetics agents in maintaining ATP status during stress. This perhaps happens due to a favorable delivery of GAA through specific membrane transporters (such as…

Comparative Surface Morphology, Chemical Composition, and Cytocompatibility of Bio-C Repair, Biodentine, and ProRoot MTA on hDPCs

2020

Biocompatibility is an essential property for any vital pulp material that may interact with the dental pulp tissues. Accordingly, this study aimed to compare the chemical composition and ultrastructural morphology of Biodentine (Septodont, Saint Maur-des-Fosses, France), ProRoot MTA (Dentsply Tulsa Dental Specialties, Johnson City, TN, USA), and Bio-C Repair (Angelus, Londrina, PR, Brazil), as well as their biological effects on human dental pulp cells. Chemical element characterization of the materials was undertaken using scanning electron microscopy and energy dispersive X-ray analysis (SEM-EDX). The cytotoxicity was assessed by analyzing the cell viability (MTT assay), cell morphology …