Search results for "Trace"
showing 10 items of 3218 documents
Trace elements in carbonate raw materials being used in cement and lime industry in Opole Province
2009
Extracellular vesicles in the human maternal-embryo crosstalk during the preconception period
2020
ANTECEDENTES: nuestro grupo demostró que el endometrio humano secreta exosomas (EXOs) al líquido endometrial (LE) con un contenido en miRNAs específico que puede ser internalizado por el embrión para favorecer la adhesión embrionaria. OBJETIVOS: definir y caracterizar los diferentes tipos de vesículas extracelulares (VEs) secretados por el endometrio humano al LE y estudiar su contenido en DNA, determinar si es internalizado por el embrión y si afecta a su regulación energética. MATERIALES Y MÉTODOS: El aislamiento de las VEs del LE se realizó mediante centrifugación diferencial. Se estudió su morfología, marcadores moleculares, tamaño y concentración a lo largo del ciclo menstrual. Su cont…
Effects of the Extraction Technology on Pomegranate Juice Quality
2020
Pomegranate juice is a rich source of phenolic components; its consumption has considerably increased throughout the world in recent years, due to its nutraceutical properties. Commercial juice production involves pressing the fruits. The aim of this study was to assess the influence of the pressing stage on pomegranate juice properties, in terms of value, duration of the applied pressure and juice yield in order to examine the influence of pressure level on volatiles and nutraceutical properties of the juice. Pomegranate fruits cv. Wonderful One were manually harvested and mechanically processed for extracting the juice by means of a shelling machine, a peristaltic pump and a pneumatic pre…
RAÍCES MITOLÓGICAS EN LA ICONOGRAFÍA DE LA BELLA DURMIENTE EL CASO DE LA BELLA DURMIENTE DE WALT DISNEY
2020
Sleeping Beauty is a story we can all tell. A cursed princess who pricks herself and falls into a deep sleep until her prince wakes her up. But, like all stories, she has evolved into this form. If we trace its origins, the first time it was put down on paper was in the 13th century. Later, in the 14th century, three more versions were added. In the 17th century Giambattista Basile popularized it, but in that same century Perrault adapted it to his own way and in the 19th century the Brothers Grimm simplified it. Through all these forms there remains an essence, a common narrative, some characteristic elements that allow us to identify this story as the same one. And in them we …
N-Methyl-β-carboline alkaloids: structure-dependent photosensitizing properties and localization in subcellular domains
2020
N-Methyl-β-carboline (βC) alkaloids, including normelinonine F (1b) and melinonine F (2b), have been found in a vast range of living species playing different biological, biomedical and/or pharmacological roles. Despite this, molecular bases of the mechanisms through which these alkaloids would exert their effect still remain unknown. Fundamental aspects including the photosensitizing properties and intracellular internalization of a selected group of N-methyl-βC alkaloids were investigated herein. Data reveal that methylation of the βC main ring enhances its photosensitizing properties either by increasing its binding affinity with DNA as a biomolecular target and/or by increasing its oxid…
Art as Inquiry: The Autopsy of an [Art] Experience
2003
In this article, I explore transgressive experience by embracing my personal encounters with art and life. In accordance with a phenomenological approach, I emphasize immediate experience, description, and analysis of the world as a source of knowledge and understanding. From the perspective both of art education and research, I attempt to show the enslaving effect of conventions. Furthermore, I trace and lay bare the origins of my transgressive experience from a methodological viewpoint by describing and interpreting the moments and threads that link them with time, place, and situations, thus making transparent my actions as a researcher. I travel upstream, first discussing writing, then…
In vitro antitumor effects of the cold-water extracts of Mediterranean species of genus Pleurotus (higher Basidiomycetes) on human colon cancer cells
2014
The aim of this study was to evaluate whether the cold-water extracts of Pleurotus eryngii var. ferulae (CWE-Pef) and Pleurotus nebrodensis (CWE-Pn), 2 of the most prized wild and cultivated edible mushrooms, can affect the tumor phenotype of human colon cancer HCT116 cells. Our results showed that treatment with CWE- Pef and CWE-Pn resulted in a significant inhibition of the viability of HCT116 cells and promoted apoptosis, as also demonstrated by the increase of Bax-to-Bcl-2 messenger RNA ratio. Moreover, we observed that both extracts were able to inhibit cell migration and to affect homotypic and heterotypic cell-cell adhesion. It also was found that treatment with CWE-Pef and CWE-Pn ne…
Mechanisms of Cell-Volume Regulation in the Central Nervous System
2017
Function and viability of every cell is dependent on the proper control of its shape and volume. In the brain, which is protected by a rigid outer shell, cell-volume regulation is of particular importance, since large volume changes affect cerebral perfusion and, hence, function. Neuronal activation leads to constant changes of the ionic and metabolic composition of the brain’s extracellular space. These changes are buffered by astrocytes on the expense of constant changes in cell volume. Under pathological conditions, the ability of astrocytes to maintain the homeostasis of the brain is overwhelmed and permanent cell swelling, cytotoxic edema, occurs. The current chapter describes the prin…
Isolation of nuclei and downstream processing of cell-type-specific nuclei from micro-dissected mouse brain regions – techniques and caveats
2020
AbstractThe mammalian brain consists of several structurally and functionally distinct regions equipped with an equally complex cell-type system. Due to its relevance in uncovering disease mechanisms, the study of cell-type-specific molecular signatures of different brain regions has increased. The rapid evolution of newer and cheaper sequencing techniques has also boosted the interest in cell-type-specific epigenetic studies. In fact, the nucleus holds most of the cell’s epigenetic information and is quite resistant to tissue dissociation processes as compared to cells. As such, nuclei are continually preferred over cells for epigenetic studies. However, the isolation of nuclei from cells …
Hsp60 in Skeletal Muscle: From Molecular Anatomy to Pathophysiology
2019
The chaperoning system of an organism is composed of the entire set of chaperones, co-chaperones, and chaperone co-factors and their interactors and receptors. Its functions pertain typically to protein homeostasis but also to many other activities inside and outside cells. In the skeletal muscle, with its multi-molecular structures rich in proteins and their continuous rearrangements, the chaperoning system plays a crucial role. However, little is known about the details of the workings of the chaperoning system in skeletal muscle development and during exercise and disease. Molecular chaperones are surely involved in muscle formation and maintenance under physiologic conditions and under …