Search results for "Trace"

Kolorektālā audzēja šūnu līniju SW480 un SW620 ekstracelulāro vezikulu endocitozes ceļa noskaidrošana monocītu šūnu līnijā THP-1

2016

Vēža šūnu izdalītās ekstracelulārās vezikulas tiek uzskatītas par vienu no audzēju mikrovides starpšūnu komunikācijas līdzekļiem, kā arī metastātiskās nišas veidošanās iniciatoriem. Makrofāgi ir būtiska audzēja mikrovides sastāvdaļa, tāpēc darba mērķis bija noteikt audzēja šūnu izdalīto ekstracelulāro vezikulu endocitozes ceļu monocītu šūnu līnijā THP-1. Kolorektālā audzēja šūnu līniju SW480 un SW620 normoksijas un hipoksijas apstākļos izdalītās ekstracelulārās vezikulas iezīmēja ar fluorescento krāsvielu Syto RNASelect un noteica to uzņemšana THP-1 šūnās ar plūsmas citometrijas metodi. Izmantojot klatrīna, kaveolīna/lipīdu mikrodomēnu, fagocitozes un makropinocitozes inhibitorus, normoksij…

Processi tradizionali e polifenoli naturali per formaggi innovativi e nutraceutici

2018

l’aggiunta di composti antiossidanti naturali nei prodotti caseari ne aumenta il valore nutrizionale e il periodo di conservabilità

Huit objets de cuivre et de bronze du piémont pyrénéen oriental : analyse croisée typologique et métallique

2015

Cet article porte sur huit pièces à base de cuivre – deux haches plates et six poignards – provenant de la zone sous-pyrénéenne orientale (Corbières, Haute Vallée de l’Aude, Pays d’OImes), dans les départements de l’Aude et de l’Ariège de l’Est (région de Lavelanet). Elles sont décrites et replacées dans leur contexte typochronologique, alors que les aspects technoéconomiques sont envisagés via la composition métallique (composition élémentaire et rapports isotopiques du plomb). Cinq objets en cuivre – deux haches et trois poignards – sont attribués au Chalcolithique. L’une des haches se rapproche des types nord-ibériques et son importation depuis cette région est suggérée par les analyses …

Closed tank pneumatic press application to improve Sauvignon Blanc wine quality and nutraceutical properties

2019

The machines used in the winemaking process directly affect the quality of wine and its nutraceutical properties. Grapes-pressing is a very important step in winemaking as it may promote the presence and/or absence of enzymatic processes on the must, leading to the creation of different products in terms of chemical composition, starting from the same grapes. The aim of the study was to compare two different pressing systems of Sauvignon Blanc grapes using an innovative pneumatic discontinuous closed tank press in two operating modes: the traditional pressing mode in presence of oxygen and the inert pressing mode, performed through grapes pressing under inert gas with nitrogen recovery. Che…

Innovative and Applied Research in Biology: Proceedings, Vol. 2

2020

The collection contains SCIENTIFIC articles on the topics of the LU 78 conference reports. The main focus is on innovative and applied research in biology and interdisciplinary fields.

Exploration of extracellular vesicles from

2018

ABSTRACT The prevalent porcine helminth, Ascaris suum, compromises pig health and reduces farm productivity worldwide. The closely related human parasite, A. lumbricoides, infects more than 800 million people representing a disease burden of 1.31 million disability-adjusted life years. The infections are often chronic in nature, and the parasites have a profound ability to modulate their hosts’ immune responses. This study provides the first in-depth characterisation of extracellular vesicles (EVs) from different developmental stages and body parts of A. suum and proposes the role of these vesicles in the host–parasite interplay. The release of EVs from the third- (L3) and fourth-stage (L4)…

Aislamiento y caracterización de vesículas extracelulares en especies del género Candida

2017

Se ha desarrollado un método de obtención de exosomas, a partir de cultivos de levaduras del género Candida, que ha permitido obtener microvesículas de elevada homogeneidad y pureza. Mediante microscopía electrónica de transmisión y técnicas de detección de nanopartículas (NTA), se ha podido constatar la presencia de microvesículas de tamaños comprendidos entre 100 y 200 nm. La caracterización de proteínas llevada a cabo por técnicas de Western blot, ha puesto de manifiesto la elevada pureza de los exosomas obtenidos, no existiendo contaminación celular en las preparaciones, tal y como lo indican el resultado positivo frente a calnexina en el lisado celular y negativo en las vesículas extra…

Microsoft Dynamics Ax 2012 ātrdarbības uzlabošana

2015

Microsoft Dynamics Ax 2012 ir jaunākais produkts no Microsoft Dynamics Ax produktu saimes. Lai jaunie un esoši klienti būtu apmierināti sistēmas, ir jānodrošina ātra un stabila tās darbība. Bakalaura darba mērķis ir Microsoft Dynamics Ax 2012 ātrdarbības analīze un uzlabošana. Darba teorētiskajā daļā tiek apskatīta Microsoft Dynamics Ax 2012 sistēmas uzbūve un ātrdarbības testēšanas rīki. Darba praktiskajā daļā tiek veikti sistēmas ātrdarbības testi, un veiktas izmaiņas sistēmas konfigurācijā un programmas kodā. Darba rezultātā tika panākts sistēmas ātrdarbības uzlabojums.

Water Dynamics in Biological Systems investigated using Neutron Scattering Techniques

Living systems can not survive in absence of the water environments which play a fundamental role in living functions. Thus in the scienti?c community many studies were and are addressed to characterize water and its dynamics properties in biological systems. However, a clear description of water in such systems has been not reached yet. In fact, the investigations performed with di?erent techniques - those based on Nuclear Magnetic Resonance or those based on Neutron Scattering - look at di?erent di?usive motions and interactions water-biomolecules, leading controversial results and hence generating many debates between scientists. In this thesis we support the idea that two water populati…

Chemical and isotopic characterization of rainwater in volcanic, urban and industrial areas of Sicily (Italy)

2021

The chemical composition of rainwater depends on (i) the dissolution of gases and particulate matter emitted by different sources, (ii) the chemical and physical reactions occurring during local and regional scale transport, and (iii) removal processes. The source of major and trace elements dissolved in rainwater can be both natural (e.g. sea salts, volcanic emissions, geogenic dust, biogenic material) and anthropogenic (e.g. industrial plants, vehicular traffic, domestic heating, vessel traffic). A group of trace elements has been recently brought to attention, especially for their possible environmental impacts, the Technology-critical elements (TCEs): Ga, Ge, In, Te, Nb, Ta, Tl, the Pla…