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showing 10 items of 1841 documents

Lactobacillus sicerae sp. nov., a lactic acid bacterium isolated from Spanish natural cider

2014

Strains CUPV261T and CUPV262 were isolated from ropy natural ciders of the Basque Country, Spain, in 2007. Cells are Gram-stain positive, non-spore-forming, motile rods, facultative anaerobes and catalase-negative. The strains are obligately homofermentative (final product dl-lactate) and produce exopolysaccharides from sucrose. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the highest similarity to both isolates corresponded to the type strain of Lactobacillus vini (99.1 %), followed by Lactobacillus satsumensis (96.4 %), and Lactobacillus oeni (96.2 %), and for all other established species, 16S rRNA gene sequence similarities were below 96 %. The species delineatio…

DNA BacterialMolecular Sequence DataBiologyMicrobiologyGenomeMicrobiologyBeveragesRNA Ribosomal 16SLactic AcidGenePhylogenyEcology Evolution Behavior and SystematicsWhole genome sequencingStrain (chemistry)Phylogenetic treeFatty AcidsSequence Analysis DNAGeneral Medicine16S ribosomal RNABacterial Typing TechniquesRandom Amplified Polymorphic DNA TechniqueRAPDLactobacillusSpainFood MicrobiologyPyrosequencingInternational Journal of Systematic and Evolutionary Microbiology
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Actibacterium mucosum gen. nov., sp. nov., a marine alphaproteobacterium from Mediterranean seawater

2012

Strain R46T, a marine alphaproteobacterium, was isolated from Mediterranean seawater at Malvarrosa beach, Valencia, Spain. It is an aerobic chemo-organotrophic, mesophilic and slightly halophilic organism, with complex ionic requirements. Phylogenetic analyses based on the 16S rRNA and gyrB gene sequences showed that strain R46T formed a separate branch within the family Rhodobacteraceae , bearing similarities below 94.7 and 80.3 %, respectively, to any other recognized species. It contained Q10 as the predominant isoprenoid quinone and C18 : 1ω7c/C18 : 1ω6c as the major cellular fatty acid. Phosphatidylglycerol was the only identified polar lipid, although other lipids were also detected. …

DNA BacterialMolecular Sequence DataBiologyMicrobiologyGenusPhylogeneticsRNA Ribosomal 16SBotanyMediterranean SeaSeawaterRhodobacteraceaePhylogenyEcology Evolution Behavior and SystematicsBase CompositionPhylogenetic treeStrain (chemistry)Fatty AcidsQuinonesPhosphatidylglycerolsSequence Analysis DNAGeneral MedicineRibosomal RNA16S ribosomal RNAHalophileBacterial Typing TechniquesType speciesSpainInternational Journal of Systematic and Evolutionary Microbiology
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Photobacterium aphoticum sp. nov., isolated from coastal water

2010

A facultatively anaerobic marine gammaproteobacterium, designated strain M46T, was isolated from Mediterranean seawater at Malvarrosa beach, Valencia, Spain. The strain was characterized by using a polyphasic approach and was found to be situated within the genus Photobacterium in the family Vibrionaceae. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46T was closely related to P. rosenbergii CECT 7644T, P. halotolerans CECT 5860T and P. ganghwense CECT 7641T, showing sequence similarities of 96.8, 96.4 and 96.2 %, respectively. According to the results of phylogenetic analyses based on recA and gyrB gene sequences, the most closely related taxon was P. ganghwens…

DNA BacterialMolecular Sequence DataMicrobiologyMicrobiologyAesculinchemistry.chemical_compoundVibrionaceaeRNA Ribosomal 16SSeawaterGenePhylogenyEcology Evolution Behavior and SystematicsBase CompositionPhylogenetic treebiologyPhotobacteriumFatty AcidsSequence Analysis DNAGeneral Medicine16S ribosomal RNAPhotobacteriumbiology.organism_classificationBacterial Typing TechniqueschemistrySpainTaxonomy (biology)BacteriaInternational Journal of Systematic and Evolutionary Microbiology
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Canibacter oris gen. nov., sp. nov., isolated from an infected human wound.

2014

A facultatively anaerobic, Gram-reaction-positive, catalase- and oxidase-negative, rod-shaped bacterium isolated from an infected human wound caused by a dog bite was characterized by phenotypic and molecular genetic methods. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain IMMIB Q2029717T was a member of the order Micrococcales of the class Actinobacteria , displaying 91.6 % to 96 % sequence similarity with members of the family Microbacteriaceae . Phylogentic trees generated by different algorithms indicated that the strain forms an independent phylogenetic line of descent that consistently clustered proximal to the base of the genus Leucobacter . Chemical studies…

DNA BacterialMolecular Sequence DataPeptidoglycanMicrobiologyRibotypingCanibacter orisMicrobiologyRibotypingDogsGenusRNA Ribosomal 16SActinomycetalesAnimalsHumansBites and StingsGeneEcology Evolution Behavior and SystematicsPhylogenyBase CompositionbiologyStrain (chemistry)Phylogenetic treeVitamin K 2General MedicineSequence Analysis DNAMiddle Agedbiology.organism_classification16S ribosomal RNABacterial Typing TechniquesFemaleBacteriaInternational journal of systematic and evolutionary microbiology
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Description of Enterovibrio nigricans sp. nov., reclassification of Vibrio calviensis as Enterovibrio calviensis comb. nov. and emended description o…

2009

Eleven strains of halophilic, facultative anaerobes isolated from healthy and diseased Dentex dentex and Sparus aurata (bony fishes) cultured in Spanish Mediterranean fisheries have been studied by a polyphasic approach that included a wide phenotypic characterization, DNA-DNA hybridization and phylogenetic analysis using 16S rRNA, recA and rpoD gene sequences. All strains were phylogenetically related to Enterovibrio species and Vibrio calviensis. On the basis of sequence analysis and DNA-DNA hybridization data, eight of the strains were identified as Enterovibrio coralii. The remaining three strains formed a tight, independent clade in all sequence analyses and showed less than 70 % DNA-D…

DNA BacterialMolecular Sequence DataVibrionaceaeSigma FactorDNA RibosomalMicrobiologyMicrobiologyVibrionaceaePhylogeneticsRNA Ribosomal 16SSequence Homology Nucleic AcidAnimalsCluster AnalysisPhylogenyEcology Evolution Behavior and SystematicsGeneticsbiologyPhylogenetic treeNucleic Acid HybridizationDentex dentexDNA-Directed RNA PolymerasesSequence Analysis DNAGeneral MedicineRibosomal RNAbiology.organism_classification16S ribosomal RNAVibrioBacterial Typing TechniquesPerciformesRec A RecombinasesSpainTaxonomy (biology)International Journal of Systematic and Evolutionary Microbiology
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Lactobacillus oeni sp. nov., from wine.

2009

Ten Lactobacillus strains, previously isolated from different Bobal grape wines from the Utiel-Requena Origin Denomination of Spain, were characterized phylogenetically, genotypically and phenotypically. The 16S rRNA genes were sequenced and phylogenetic analysis showed that they form a tight phylogenetic clade that is closely related to reference strains Lactobacillus satsumensis NRIC 0604T, ‘Lactobacillus uvarum’ 8 and Lactobacillus mali DSM 20444T. DNA–DNA hybridization results confirmed the separation of the strains from other Lactobacillus species. Genotypically, the strains could be differentiated from their closest neighbours by 16S amplified rDNA restriction analysis and random ampl…

DNA BacterialMolecular Sequence DataWineMicrobiologyDNA RibosomalMicrobiologyAesculinchemistry.chemical_compoundPhylogeneticsLactobacillusRNA Ribosomal 16SGenotypeCluster AnalysisAnaerobiosisEcology Evolution Behavior and SystematicsPhylogenyWinebiologyPhylogenetic treefood and beveragesNucleic Acid HybridizationGeneral MedicineSequence Analysis DNAbiology.organism_classification16S ribosomal RNACatalaseDNA FingerprintingBacterial Typing TechniquesRandom Amplified Polymorphic DNA TechniqueLactobacilluschemistrySpainBacteriaPolymorphism Restriction Fragment LengthInternational journal of systematic and evolutionary microbiology
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Mucilaginibacter frigoritolerans sp. nov., Mucilaginibacter lappiensis sp. nov. and Mucilaginibacter mallensis sp. nov., isolated from soil and liche…

2010

Five cold-adapted bacteria belonging to the genus Mucilaginibacter were isolated from lichen and soil samples collected from Finnish Lapland and investigated in detail by phenotypic and phylogenetic analyses. Based on 16S rRNA gene phylogeny, the novel strains represent three new branches within the genus Mucilaginibacter. The strains were aerobic, chemo-organotrophic, non-motile rods and formed pigmented, smooth, mucoid colonies on solid media. The strains grew between 0 and 33 °C (optimum growth at 25 °C) and at pH 4.5–8.0 (optimum growth at pH 6.0). The main cellular fatty acids were iso-C15 : 0, summed feature 3 (C16 : 1 ω7c/iso-C15 : 0 2-OH) and iso-C17 : 0 3-OH and the major respirato…

DNA BacterialMucilaginibacter frigoritoleransfood.ingredientLichensMolecular Sequence DataBiologymedicine.disease_causeMicrobiologyMicrobiologyfoodPhylogeneticsRNA Ribosomal 16SBotanymedicineLichenEcology Evolution Behavior and SystematicsFinlandPhylogenySoil MicrobiologyBase CompositionMucilaginibacter mallensisPhylogenetic treeBacteroidetesFatty AcidsMucilaginibacterVitamin K 2General MedicineSequence Analysis DNA16S ribosomal RNABacterial Typing TechniquesMucilaginibacter lappiensisInternational journal of systematic and evolutionary microbiology
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Neptuniibacter caesariensis gen. nov., sp. nov., a novel marine genome-sequenced gammaproteobacterium.

2007

7 pages, 1 figure, 1 table

DNA BacterialOceanospirillaceaeSequence analysisMovementMolecular Sequence DataCarboxylic AcidsZoologyAlteromonadaceaeMicrobiologyDNA RibosomalMicrobiologyMembrane LipidsPhylogeneticsRNA Ribosomal 16SSequence Homology Nucleic AcidMediterranean SeaSeawaterAmino AcidsEcology Evolution Behavior and SystematicsPhylogenyBase CompositionbiologyPhylogenetic treeAlteromonadaceaeFatty AcidsGenes rRNAGeneral MedicineSequence Analysis DNARibosomal RNAbiology.organism_classification16S ribosomal RNAHalophileBacterial Typing TechniquesRNA BacterialOceanospirillaceaeGammaproteobacteriaGenome BacterialInternational journal of systematic and evolutionary microbiology
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Streptococcus lactarius sp. nov., isolated from breast milk of healthy women.

2011

Three strains of a hitherto-unknown, Gram-stain-positive coccus were recovered from the milk of three non-related healthy women. The isolates shared 99% 16S rRNA gene sequence similarity with sequences from uncultured members of the Lactobacillales and Streptococcus. The closest sequence corresponding to a defined species was that of Streptococcus peroris GTC 848T, with a similarity of 98%. A partial sequence (488 bp) of the tuf gene also showed 97% similarity with that of S. peroris CCUG 39814T. The combined 16S rRNA/tuf-based phylogeny revealed that all the isolates grouped in a statistically well-supported cluster separate from S. peroris. Enzyme activity profiles as well as fermentation…

DNA BacterialPhylogenetic treebiologyMilk HumanStreptococcusLactobacillalesMolecular Sequence DataBacterialStreptococcusGeneral Medicine16S ribosomal RNAmedicine.disease_causebiology.organism_classificationStreptococcaceaeMicrobiologyMicrobiologyStreptococcus perorisStreptococcus mitisLactariusRNA Ribosomal 16SmedicineHumansFemaleEcology Evolution Behavior and SystematicsPhylogenyInternational journal of systematic and evolutionary microbiology
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Polyphyletic Origin of Vibrio vulnificus Biotype 2 as Revealed by Sequence-Based Analysis ▿ †

2011

ABSTRACT A sequence-based analysis of seven housekeeping and virulence-related genes shows that the species Vibrio vulnificus is subdivided into three phylogenetic lineages that do not correspond with the biotypes and that biotype 2 is polyphyletic. These results support the reclassification of biotype 2 as a pathovar that would group the strains with pathogenic potential to develop vibriosis in fish.

DNA BacterialPolymorphism GeneticEcologyPhylogenetic treeVibrio vulnificusPublic Health MicrobiologyBiologybiology.organism_classificationApplied Microbiology and BiotechnologyBacterisMicrobiologyBacterial Typing TechniquesPathovarVibrionaceaePhylogeneticsPolyphylyAnimalsGeneVibrio vulnificusPhylogenyFood ScienceBiotechnologySequence (medicine)
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