Search results for "Tris"
showing 10 items of 2410 documents
Potential for managing life history diversity in a commercially exploited intermediate predator, the goldsinny wrasse (Ctenolabrus rupestris)
2019
Abstract Small-bodied wrasse species are important for structuring coastal marine ecosystems but are also increasingly harvested as parasite cleaners on farmed salmon. Identifying management regulations that will support long-term sustainability of wrasse fisheries is challenging, because there is still limited knowledge about the impacts of fisheries on the demography of these intermediate predators in their natural environments. To this end, we studied individual growth histories of goldsinny wrasse (Ctenolabrus rupestris) at a fine spatial scale across replicated marine protected areas (MPAs) and areas open to commercial harvesting on the Norwegian coast. The MPAs were established 1–7 ye…
Effects of local forest continuity on the diversity of fungi on standing dead pines
2018
Human-induced fragmentation affects forest continuity, i.e. availability of a suitable habitat for the target species over a time period. The dependence of wood-inhabiting fungi on landscape level continuity has been well demonstrated, but the importance of local continuity has remained controversial. In this study, we explored the effects of local forest continuity (microhabitat and stand level) on the diversity of wood-inhabiting fungi on standing dead trunks of Scots pine (Pinus sylvestris L.). We studied species richness and community composition of decomposers and Micarea lichens on 70 trunks in 14 forests in central Finland that differed in their state of continuity. We used dendrochr…
An Egg Parasitoid Efficiently Exploits Cues From a Coevolved Host But Not Those From a Novel Host
2019
Egg parasitoids have evolved adaptations to exploit host-associated cues, especially oviposition-induced plant volatiles and odors of gravid females, when foraging for hosts. The entire host selection process is critical for successful parasitism and relevant in defining host specificity of parasitoids. We hypothesized that naïve egg parasitoid females reared on their coevolved host are able to exploit cues related to the coevolved host but not those from a novel host. We used the egg parasitoid Trissolcus japonicus, its coevolved host Halyomorpha halys, and the non-coevolved host Podisus maculiventris to evaluate this hypothesis. H. halys, a polyphagous pest native from Eastern Asia, has i…
Conceptual Overview of an Anthropocentric Training Station for Manual Operations in Production
2019
Abstract The paper presents a conceptual overview of a human-centred training station for manual operations (ATASMO). It identifies the main users of the system but also the long-tern targeted features of ATASMO. Moreover, the current implementation, its limitations and future work on ATASMO is synthetically presented.
Transcriptomic behavior of genes associated with chromosome 21 aneuploidies in early embryo development.
2019
To analyze how chromosome 21 (HSA21) ploidy affects global gene expression of early human blastocysts.Prospective study.University-affiliated in vitro fertilization clinic.A total of 26 high-quality donated embryos from in vitro fertilization (IVF) patients: trisomy 21 (n = 8), monosomy 21 (n = 10), and euploid (n = 8) blastocysts.None.Blastocyst transcriptome changes and its associated functions.Trisomy 21, monosomy 21, and euploid blastocysts were classified by comparative genomic hybridization. The global transcriptome of whole blastocysts was analyzed with small cell number RNA sequencing, and they were compared to understand the gene expression behavior at early development and its imp…
A Short and Improved Synthesis of the Antiprotozoal Abietane Diterpenoid (–)-Sugikurojin A
2016
An efficient and straightforward semisynthesis of the antiprotozoal abietane diterpenoid (–)-sugikurojin A, starting from the readily available methyl ester of callitrisic acid (4-epi-dehydroabietic acid) isolated from sandarac resin, is reported. This optimized semisynthetic route provides a convenient source of the antiprotozoal compound, in four steps from methyl callitrisate in 50% overall yield, for further biological studies.
Yeast Cth2 protein represses the translation of ARE-containing mRNAs in response to iron deficiency
2018
In response to iron deficiency, the budding yeast Saccharomyces cerevisiae undergoes a metabolic remodeling in order to optimize iron utilization. The tandem zinc finger (TZF)-containing protein Cth2 plays a critical role in this adaptation by binding and promoting the degradation of multiple mRNAs that contain AU-rich elements (AREs). Here, we demonstrate that Cth2 also functions as a translational repressor of its target mRNAs. By complementary approaches, we demonstrate that Cth2 protein inhibits the translation of SDH4, which encodes a subunit of succinate dehydrogenase, and CTH2 mRNAs in response to iron depletion. Both the AREs within SDH4 and CTH2 transcripts, and the Cth2 TZF are es…
mRNA-binding protein tristetraprolin is essential for cardiac response to iron deficiency by regulating mitochondrial function
2018
Cells respond to iron deficiency by activating iron-regulatory proteins to increase cellular iron uptake and availability. However, it is not clear how cells adapt to conditions when cellular iron uptake does not fully match iron demand. Here, we show that the mRNA-binding protein tristetraprolin (TTP) is induced by iron deficiency and degrades mRNAs of mitochondrial Fe/S-cluster-containing proteins, specifically Ndufs1 in complex I and Uqcrfs1 in complex III, to match the decrease in Fe/S-cluster availability. In the absence of TTP, Uqcrfs1 levels are not decreased in iron deficiency, resulting in nonfunctional complex III, electron leakage, and oxidative damage. Mice with deletion of Ttp …
GH57 amylopullulanase from Desulfurococcus amylolyticus JCM 9188 can make highly branched cyclodextrin via its transglycosylation activity.
2018
Abstract Desulfurococcus amylolyticus is an anaerobic and hyperthermophilic crenarchaeon that can use various carbohydrates as energy sources. We found a gene encoding a glycoside hydrolase family 57 amylolytic enzymes (DApu) in a putative carbohydrate utilization gene cluster in the genome of D. amylolyticus . This gene has an open reading frame of 1,878 bp and consists of 626 amino acids with a molecular mass of 71 kDa. Recombinant DApu (rDApu) completely hydrolyzed pullulan to maltotriose by attacking α-1,6-glycosidic linkages, and was able to produce glucose and maltose from soluble starch and amylopectin. Although rDApu showed no activity toward α-cyclodextrin (CD) and β-CD, maltooctao…
The extracellular wall-bound β-N-acetylglucosaminidase from Lactobacillus casei is involved in the metabolism of the human milk oligosaccharide lacto…
2015
Human milk oligosaccharides (HMOs) are considered to play a key role in establishing and maintaining the infant gut microbiota. Lacto-N-triose forms part of both type 1 and type 2 HMOs and also of the glycan moieties of glycoproteins. Upstream of the previously characterized gene cluster involved in lacto-N-biose and galacto-N-biose metabolism from Lactobacillus casei BL23, there are two genes, bnaG and manA, encoding a β-N-acetylglucosaminidase precursor and a mannose-6-phosphate isomerase, respectively. In this work, we show that L. casei is able to grow in the presence of lacto-N-triose as a carbon source. Inactivation of bnaG abolished the growth of L. casei on this oligosaccharide, dem…