Search results for "Trolox"
showing 10 items of 46 documents
In vitro bioactive properties of phlorotannins recovered from hydrothermal treatment of Sargassum muticum
2016
Abstract The present work addresses the potential of different adsorbents for the recovery of the phlorotannin fraction present in the liquid phase from the autohydrolysis of Sargassum muticum. Operating with the extract generated during autohydrolysis of alginate exhausted algae, the kinetics followed pseudo-second order and the equilibrium corresponded to Freundlich model. In batch configuration, up to 70% phlorotannins were adsorbed and up to 30% were desorbed with ethanolic solutions. Comparable performance was observed with the autohydrolysis extracts from the whole algae. The phloroglucinol content, the reducing and ABTS antiradical capacities of the desorbed product increased by 2–4 …
Wild
2021
Graphical abstract
Antioxidant activity and phenolic composition in pomegranate (Punica granatumL.) genotypes from south Italy by UHPLC-Orbitrap-MS approach
2018
Background: Pomegranate fruits are a rich source of polyphenols with numerous health-promoting effects. Pomegranate juices of five genotypes ('Mollar', 'Kingdom', 'Dente di Cavallo', and two old populations 'Francofonte' and 'Santa Tecla') were evaluated regarding anthocyanin and non-anthocyanin phenolic contents using ultrahigh performance liquid chromatography (UHPLC)-Orbitrap-mass spectrometry (MS). Moreover, total antioxidant activity (TAA) was evaluated using a 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) assay.; Results: Twenty-three phenolic compounds were identified. Cyanidin-3,5-O-diglucoside and pelargonidin-3,5-O-diglucoside were the most represent…
Improved bioaccessibility and antioxidant capacity of olive leaf ( Olea europaea L.) polyphenols through biosorption on Saccharomyces cerevisiae
2016
Abstract Olive ( Olea europaea L.) leaves (OL) are natural by-products that can be used as an advantageous rich source of bioactive compounds, especially polyphenols. This study investigated the potential use of Saccharomyces cerevisiae as a new carrier of OL antioxidants and a means to protect their bioactivity during simulated gastrointestinal digestion. The biosorption on S. cerevisiae allowed the recovery of 25.17 mg/g and 49.40% of the polyphenols from olive leaf (var. Chemlali ) infusions. Both infusions and suspensions before and after biosorption were analyzed for total soluble polyphenols and total antioxidant capacity (oxygen radical absorbance capacity (ORAC) and trolox equivalen…
Impaired plasma nitric oxide availability and extracellular superoxide dismutase activity in healthy humans with advancing age
2006
This study is aimed to verify the modifications of extracellular superoxide dismutase (EC-SOD) activity and its potential involvement on the mechanism responsible for the impairment of plasma nitric oxide (NO) availability occurring with advancing age in healthy humans. For this purpose, plasma samples were drawn from 40 healthy men, aged 20-92 years, in fasting state and used for measurements of stable end-product nitrite/nitrate (NOx), as expression of NO availability, EC-SOD activity, thiobarbituric acid reactive substances (TBARS) as marker of lipid peroxidation, Trolox equivalent antioxidant capacity (TEAC) as a measure of plasma total antioxidant capacity, and in vitro susceptibility …
Different effectiveness of two pastas supplemented with either lipophilic or hydrophilic/phenolic antioxidants in affecting serum as evaluated by the…
2017
Effectiveness in improving serum antioxidant status of two functional pastas was evaluated by the novel Antioxidant/ Oxidant Balance (AOB) parameter, calculated as Antioxidant Capacity (AC)/Peroxide Level ratio, assessed here for the first time. In particular, Bran Oleoresin (BO) and Bran Water (BW) pastas, enriched respectively with either lipophilic (tocochromanols, carotenoids) or hydrophilic/phenolic antioxidants extracted from durum wheat bran, were studied. Notably, BO pasta was able to improve significantly (+65%) serum AOB during four hours after intake similarly to Lisosan G, a wheat antioxidant-rich dietary supplement. Contrarily, BW pasta had oxidative effect on serum so as conve…
Evaluation of the antioxidant capacity, furan compounds and cytoprotective/cytotoxic effects upon Caco-2 cells of commercial Colombian coffee.
2017
Abstract Antioxidant capacity (AC), total phenolics (TPs), furan compounds (HMF and furfural F) and cytoprotective/cytotoxic effects upon Caco-2 cells (MTT, cell cycle and reactive oxygen species (ROS)) were evaluated in Colombian coffee (2 ground and 4 soluble samples). The AC (ABTS and FRAP), TPs and HMF ranged between 124–722, 95–802 μmoles Trolox/g, 21–100 mg gallic acid/g and 69–2900 mg/kg, respectively. Pretreatment of cells for 24 h with lyophilized coffee infusions at the highest dose without cytotoxic effects (500 μg/mL) significantly prevented the decrease in cell viability compared to control stress with H2O2 (5 mM/2 h), recovering viability to values between 34% and 45% and rest…
Cold plasma processing of powdered Spirulina algae for spore inactivation and preservation of bioactive compounds
2020
Abstract Technologies for controlling microbial risks in a heat and humidity sensitive food powder are still limited. To preserve bioactive compounds while inactivating Bacillus subtilis spores in powdered Spirulina microalgae (Arthrospira platensis) with a non-thermal atmospheric plasma is the challenge presented in this paper. Artificially contaminated powder was treated with a custom-made surface micro-discharge cold atmospheric pressure plasma (SMD-CAPP) at the effective, specific surface energy of the plasma (Es) of 7–15 mW/cm2. The inactivation of spores in air plasma was faster than in nitrogen plasma. The final effect after 5 min exposure time of close to 2 log10 reduction could be …
Methods used to evaluate the peroxyl (ROO·) radical scavenging capacities of four common antioxidants
2012
Different methods that are used to measure peroxyl radical (ROO) scavenging capacity were used in model systems containing four common antioxidant compounds: ascorbic acid, cysteine, gallic acid and Trolox, a water-soluble vitamin E analogue. Five tests were performed, with differences in the method of peroxyl radical generation (lipoxygenase, azo-compound thermal degradation and spontaneous oxidation from a natural source of fatty acids) and in the addition of probes such as linoleic acid, crocin, fluorescein and electron paramagnetic resonance (EPR) by spin-trapping with a-phenyl-N-tert-butylnitrone. Significant differences were found in antioxidant reactivity, evaluated by dose–effect se…
Potential in vitro antioxidant, anti-inflammatory, antidiabetic, and anticancer effect of arachidonic acid-elicited basil leaves
2017
Abstract The study presents the impact of elicitation of basil with 0.01 µM (AA1), 1 µM (AA2) and 100 µM (AA3) arachidonic acid on the content and bioactivity of potentially bioavailable polyphenols. Elevated levels of phenolic compounds correlated with increased biological activity were observed in the extracts from basil (control and elicited) subjected to simulated gastrointestinal digestion in comparison to chemical and standard buffer extracts. Elicitation with AA1 and AA2 resulted in an 3-fold and more than 4-fold increase in the content of potentially bioavailable rosmarinic acid, respectively. In some cases, elicitation with arachidonic acid improved the pro-health properties of the…