Search results for "Two-photon excitation microscopy"

Absolute frequency measurement of rubidium 5S-7S two-photon transitions.

2013

We report the absolute frequency measurements of rubidium 5S-7S two-photon transitions with a cw laser digitally locked to an atomic transition and referenced to an optical frequency comb. The narrow, two-photon transition, 5S-7S (760 nm) insensitive to first order in a magnetic field, is a promising candidate for frequency reference. The performed tests yield the transition frequency with accuracy better than reported previously.

Collinear two-photon excitation of indium rydberg states in a fast atomic beam

1986

The 29p-Rydberg state of neutral indium was produced by double resonant two-photon excitation in a fast atomic beam and detected via field ionization in a longitudinal electric field.

Two-photon microscopy and neuroinflammation: Crosstalk between T cells and neurons

2014

Dominance of the first excitation step for magnetic circular dichroism in near-threshold two-photon photoemission

2012

Magnetic circular dichroism (MCD) in near-threshold photoemission is measured for a perpendicularly magnetized Cs/Co/Pt(111) film with work function adjusted by Cs adsorption. For one-photon photoe ...

Quasi-isotropic 3-D resolution in two-photon scanning microscopy.

2009

One of the main challenges in three-dimensional microscopy is to overcome the lack of isotropy of the spatial resolution, which results from the axially-elongated shape of the point spread function. Such anisotropy gives rise to images in which significant axially-oriented structures of the sample are not resolved. In this paper we achieve an important improvement in z resolution in two-photon excitation microscopy through spatial modulation of the incident beam. Specifically, we demonstrate that the design and implementation of a simple shaded ring performs quasi-isotropic three-dimensional imaging and that the corresponding loss in luminosity can be easily compensated by most available fe…

Single-shot generation and detection of a two-photon generalized binomial state in a cavity

2006

A "quasi-deterministic" scheme to generate a two-photon generalized binomial state in a single-mode high-Q cavity is proposed. We also suggest a single-shot scheme to measure the generated state based on a probe two-level atom that "reads" the cavity field. The possibility of implementing the schemes is discussed.

Two-photon exchange correction in elastic unpolarized electron-proton scattering at small momentum transfer

2015

We evaluate the two-photon exchange (TPE) correction to the unpolarized elastic electron-proton scattering at small momentum transfer $ Q^2 $. We account for the inelastic intermediate states approximating the double virtual Compton scattering by the unpolarized forward virtual Compton scattering. The unpolarized proton structure functions are used as input for the numerical evaluation of the inelastic contribution. Our calculation reproduces the leading terms in the $ Q^2 $ expansion of the TPE correction and goes beyond this approximation by keeping the full $ Q^2 $ dependence of the proton structure functions. In the range of small momentum transfer, our result is in good agreement with …

Two-photon spectroscopy of mercury and velocity-selective double resonances

2009

Two-photon laser spectroscopy of the $6\text{ }{^{1}S}_{0}\ensuremath{-}7\text{ }{^{1}S}_{0}$ transition in mercury has been performed using two copropagating continuous-wave laser beams. One laser beam is at 254 nm wavelength and can be tuned to the $6\text{ }{^{1}S}_{0}\ensuremath{-}6\text{ }{^{3}P}_{1}$ resonance. The other laser beam is at 408 nm. Two very different regimes can be distinguished, one far off resonance and one near resonance with the one-photon resonance. A resonance which is not Doppler broadened has been observed for low Rabi frequencies. This velocity-selective double resonance in a three-level ladder system is analogous to the dark resonance in three-level $\ensuremat…

Operation conditions and stability of a degenerate two-photon laser

1993

Abstract The presence of a far off-resonant intermediate level in a degenerate three-level two-photon laser model manifests through a steady pump-dependent and a dynamic frequency shift on the laser equations. The steady shift strongly affects the pump threshold necessary for emission, preventing it in certain cases. The dynamic shift makes the frequency pulling/pushing to be intensity-dependent and substantially modify the domains of existence and stability of the lasing solution permitting stable operation for large cavity losses in certain cases.

Quasi-spherical focal spot in two-photon scanning microscopy by three-ring apodization

2005

International audience; We present a beam-shaping technique for two-photon excitation (TPE) fluorescence microscopy. We show that by inserting a properly designed three-ring pupil filter in the illumination beam of the microscope, the effective optical sectioning capacity of such a system improves so that the point spread function gets a quasi-spherical shape. Such an improvement, which allows the acquisition of 3D images with isotropic quality, is obtained at the expense of only a small increase of the overall energy in the axial sidelobes. The performance of this technique is illustrated with a scanning TPE microscopy experiment in which the image of small beads is obtained. We demonstrat…