Search results for "Two-photon"
showing 10 items of 90 documents
Quasi-isotropic 3-D resolution in two-photon scanning microscopy.
2009
One of the main challenges in three-dimensional microscopy is to overcome the lack of isotropy of the spatial resolution, which results from the axially-elongated shape of the point spread function. Such anisotropy gives rise to images in which significant axially-oriented structures of the sample are not resolved. In this paper we achieve an important improvement in z resolution in two-photon excitation microscopy through spatial modulation of the incident beam. Specifically, we demonstrate that the design and implementation of a simple shaded ring performs quasi-isotropic three-dimensional imaging and that the corresponding loss in luminosity can be easily compensated by most available fe…
Single-shot generation and detection of a two-photon generalized binomial state in a cavity
2006
A "quasi-deterministic" scheme to generate a two-photon generalized binomial state in a single-mode high-Q cavity is proposed. We also suggest a single-shot scheme to measure the generated state based on a probe two-level atom that "reads" the cavity field. The possibility of implementing the schemes is discussed.
Two-photon exchange correction in elastic unpolarized electron-proton scattering at small momentum transfer
2015
We evaluate the two-photon exchange (TPE) correction to the unpolarized elastic electron-proton scattering at small momentum transfer $ Q^2 $. We account for the inelastic intermediate states approximating the double virtual Compton scattering by the unpolarized forward virtual Compton scattering. The unpolarized proton structure functions are used as input for the numerical evaluation of the inelastic contribution. Our calculation reproduces the leading terms in the $ Q^2 $ expansion of the TPE correction and goes beyond this approximation by keeping the full $ Q^2 $ dependence of the proton structure functions. In the range of small momentum transfer, our result is in good agreement with …
Topological protection of highly entangled non-Gaussian two-photon states
2021
Abstract We study theoretically the evolution of entangled non-Gaussian two-photon states in disordered topological lattices. Specifically, we consider spatially entangled two-photon states, modulated by Laguerre polynomials up to the 3rd order, which feature ring-shaped spatial and spectral correlation patterns. Such states are discrete analogs of photon-subtracted squeezed states, which are ubiquitous in optical quantum information processing or sensing applications. We find that, in general, a higher degree of entanglement coincides with a loss of topological protection against disorder, this is in line with previous results for Gaussian two-photon states. However, we identify a particul…
Two-photon spectroscopy of mercury and velocity-selective double resonances
2009
Two-photon laser spectroscopy of the $6\text{ }{^{1}S}_{0}\ensuremath{-}7\text{ }{^{1}S}_{0}$ transition in mercury has been performed using two copropagating continuous-wave laser beams. One laser beam is at 254 nm wavelength and can be tuned to the $6\text{ }{^{1}S}_{0}\ensuremath{-}6\text{ }{^{3}P}_{1}$ resonance. The other laser beam is at 408 nm. Two very different regimes can be distinguished, one far off resonance and one near resonance with the one-photon resonance. A resonance which is not Doppler broadened has been observed for low Rabi frequencies. This velocity-selective double resonance in a three-level ladder system is analogous to the dark resonance in three-level $\ensuremat…
Operation conditions and stability of a degenerate two-photon laser
1993
Abstract The presence of a far off-resonant intermediate level in a degenerate three-level two-photon laser model manifests through a steady pump-dependent and a dynamic frequency shift on the laser equations. The steady shift strongly affects the pump threshold necessary for emission, preventing it in certain cases. The dynamic shift makes the frequency pulling/pushing to be intensity-dependent and substantially modify the domains of existence and stability of the lasing solution permitting stable operation for large cavity losses in certain cases.
The role of nonlinear optical absorption in narrowband difference-frequency terahertz-wave generation
2010
We present a general analysis of the influence of nonlinear optical absorption on terahertz generation via optical difference frequency generation, when reaching for the quantum conversion efficiency limit. By casting the equations governing the process in a suitably normalized form, including either two-photon- or three-photon-absorption terms, we have been able to plot universal charts for phase matched optical-to-terahertz conversion for different values of the nonlinear absorption coefficients. We apply our analysis to some experiments reported to date, in order to understand to what extent multiphoton absorption could have played a role and also to predict the maximum achievable conver…
Quasi-spherical focal spot in two-photon scanning microscopy by three-ring apodization
2005
International audience; We present a beam-shaping technique for two-photon excitation (TPE) fluorescence microscopy. We show that by inserting a properly designed three-ring pupil filter in the illumination beam of the microscope, the effective optical sectioning capacity of such a system improves so that the point spread function gets a quasi-spherical shape. Such an improvement, which allows the acquisition of 3D images with isotropic quality, is obtained at the expense of only a small increase of the overall energy in the axial sidelobes. The performance of this technique is illustrated with a scanning TPE microscopy experiment in which the image of small beads is obtained. We demonstrat…
Light Sheet Fluorescence Microscopy (LSFM) for Two-Photon Excitation Imaging of Thick Samples.
2015
Over the last decades, fluorescence microscopy techniques have been developed in order to provide a deeper, faster and higher resolution imaging of three-dimensional biological samples. Within this framework, Light Sheet Fluorescence Microscopy (LSFM) became an increasingly useful and popular imaging technique able to answer several biological questions in the field of developmental biology [1]. Thanks to the spatial confinement of the excitation process within a thin sheet in the focal plane, it provides an intrinsic optical sectioning and a reduced phototoxicity. On the other side, Two-Photon Excitation (2PE), thanks to the use of IR wavelengths, has become an invaluable tool to improve i…
Gold nanostars coated with neutral and charged polyethylene glycols: A comparative study of in-vitro biocompatibility and of their interaction with S…
2015
Gold nanostars (GNS) have been coated with four different polyethylene glycols (PEGs) equipped with a -SH function for grafting on the gold surface. These PEGs have different chain lengths with average MW = 2000, 3000, 5000 and average number of -O-CH2-CH2 - units 44, 66, and 111, respectively. Two are neutral and two are terminated with -COOH and -NH2 functions, thus bearing negative and positive charges at physiological pH, thanks to the formation of carboxylate and ammonium groups. The negative charge of the GNS coated with PEG carboxylate has also been exploited to further coat the GNS with the PAH (polyallylamine hydrochloride) cationic polymer. Vitality tests have been carried out on …