Search results for "Typing"
showing 10 items of 1051 documents
Human Leukocyte Antigen Typing Using High-Throughput DNA and RNA Sequencing and Application for Cell Line Identification
2019
Genome-wide detection of copy-number variations in local cattle breeds
2019
The aim of the present study was to identify copy-number variations (CNVs) in Cinisara (CIN) and Modicana (MOD) cattle breeds on the basis of signal intensity (logR ratio) and B allele frequency of each marker, using Illumina’s BovineSNP50K Genotyping BeadChip. The CNVs were detected with the PennCNV and SVS 8.7.0 software and were aggregated into CNV regions (CNVRs). PennCNV identified 487 CNVs in CIN that aggregated into 86 CNVRs, and 424 CNVs in MOD that aggregated into 81 CNVRs. SVS identified a total of 207 CNVs in CIN that aggregated into 39 CNVRs, and 181 CNVs in MOD that aggregated into 41 CNVRs. The CNVRs identified with the two softwares contained 29 common CNVRs in CIN and 17 co…
Serological and molecular characteristics of Vibrio vulnificus biotype 3: evidence for high clonality.
2007
Vibrio vulnificus biotype 3 has been implicated as the causative pathogen of an ongoing disease outbreak that erupted in Israel in 1996. Recent work based on multi-locus sequence typing (MLST) showed that V. vulnificus biotype 3 is genetically homogeneous. The aim of this study was to investigate the existence of subpopulations within this homogeneous biotype by characterizing the surface antigens and analysing the sequence diversity of selected outer-membrane protein (OMP)-encoding genes. Rabbit antisera were prepared against biotype 1, 2 and 3 strains. The results of the slide-agglutination test, dot-blot assay (using fresh and boiled cells), and immunoblotting of lipopolysaccharides (LPS…
MRSA ST22-IVa (EMRSA-15 clone) in Palermo, Italy
2010
Summary: Epidemic spread of methicillin-resistant Staphylococcus aureus (MRSA) strains carrying the Staphylococcal Chromosomal Cassettes (SCC) mec type IV is being increasingly reported in many geographical areas. A survey to determine the prevalence and characteristics of MRSA SCCmec IV isolates identified in four general hospitals in Palermo, Italy, was carried out. During the period February–June 2009, SCCmec type IVa has been found in 12 out of 94 isolates. Nine isolates from all hospitals and all strains from a NICU outbreak occurring in the same period were attributed with the ST22-IVa (EMRSA-15) clone. In our setting, due to the changing MRSA epidemiology, detection of SCCmec IV coul…
Epidemic dissemination of Salmonella enterica spp. enterica serovar Bovismorbificans in southern Italy in the years 1989-1991.
1994
Epidemic strains of Salmonella enterica subsp. enterica serovar Bovismorbificans isolated in southern Italy during the years 1989-1991 were submitted to a molecular epidemiological study in comparison with isolates identified in the years 1980-1988 in the same geographic area. Genomic DNA fragments obtained by digestion with BglI or Eco RI hybridized with Escherichia coli rRNA to produce three distinct, but highly related patterns. Ribotype 1, which had never been identified before 1989, was found to characterize most of the strains identified between 1989 and 1991. Such a finding supports the hypothesis of emergence and spread of a new bacterial clone associated with the increased number o…
An Optimized Architecture for CGA Operations and Its Application to a Simulated Robotic Arm
2022
Conformal geometric algebra (CGA) is a new geometric computation tool that is attracting growing attention in many research fields, such as computer graphics, robotics, and computer vision. Regarding the robotic applications, new approaches based on CGA have been proposed to efficiently solve problems as the inverse kinematics and grasping of a robotic arm. The hardware acceleration of CGA operations is required to meet real-time performance requirements in embedded robotic platforms. In this paper, we present a novel embedded coprocessor for accelerating CGA operations in robotic tasks. Two robotic algorithms, namely, inverse kinematics and grasping of a human-arm-like kinematics chain, ar…
Langer-Giedion syndrome with interstitial 8q-deletion.
1982
We describe a 12-year-old girl with Langer-Giedion syndrome (tricho-rhino-phalangeal syndrome type II) who also had vertebral malformations. Chromosome analysis identified an interstitial del(8q): 46,XX,del(8)(pter leads to q22::q234 leads to qter) as a cause of this syndrome.
The conjoint conceptualization and negotiation of national cultures : qualitative study of discursive culture-concepts, their function and their rela…
2015
The study of stereotypes and stereotyping is as complex and multi-facetted as the social phenomenon itself. From being seen as an erroneous and over-generalizing image in the mind of individuals, to the current perspective that stereotypes have an individual and a social level, are shaped by long-term and short-term contextual factors, and are used for certain purposes, managed and negotiated, stereotypes have been acknowledged as a topic which cannot be studied within one academic discipline alone. This study looks into stereotype use and management during in-group discourse about other national cultures. The data consists of three focus groups consisting of Finnish students who talk about…
Metodo per la genotipizzazione di HIV-1 e relativi kit diagnostici per la rilevazione di farmaco-resistenza (gene ENV-gp41).
2007
DESCRIPTION OF THE INVENTION This invention concerns a laboratory method for genotyping human immunodeficiency virus type 1 (HIV-1) through gene sequencing of gp41-env. It allows to detect the nucleotide mutations conferring resistance to antiretroviral drugs active to gp41-env. The present method allows to analyze also low viraemia samples at around 101 -102 copies HIV-RNA/ml. This method offers the advantage of being easily adaptable to both plasma and cellular samples and is therefore able to value "in advance" the genetic mutations potentially involved in the resistance phenomena to anti-retroviral drugs, even in patients with optimal/sub-optimal virologic control. The kit use the one-s…
Metodo per la genotipizzazione di HIV-1 e relativi kit diagnostici per la rilevazione di farmaco-resistenza (geni GAG/POL).
2007
DESCRIPTION OF THE INVENTION This invention concerns a laboratory method for genotyping human immunodeficiency virus type 1 (HIV-1) through gene sequencing of pol. It allows to detect the nucleotide mutations conferring resistance to antiretroviral drugs active to pol. The present method allows to analyze also low viraemia samples at around 101 -102 copies HIV-RNA/ml. This method offers the advantage of being easily adaptable to both plasma and cellular samples and is therefore able to value "in advance" the genetic mutations potentially involved in the resistance phenomena to anti-retroviral drugs, even in patients with optimal/sub-optimal virologic control. The kit use the one-step RT-PCR…