Search results for "UV-254"

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HPLC-SEC: a new approach to characterise complex wastewater effluents

2016

ABSTRACTThis work investigates the use of HPLC-SEC to characterise dissolved organic matter (DOM) of complex wastewater effluents. A silica-based column, sodium acetate eluent and multiple detections were employed: UV-254 absorbance for humictype, and tryptophan-like (Ex/Em = 270/355) and tyrosine-like (Ex/Em = 270/310) fluorescence for protein type compounds. Effects of eluent pH, eluent ionic strength and injection volume on separation efficiency were tested. Humic-type and protein-type fractions were clearly differentiated and eluted within and out of calibration range. Eluent ionic strength had the greatest influence on global resolution; the lowest eluent concentration of 0.01 M produc…

Health Toxicology and Mutagenesista1172Soil Science010501 environmental sciences01 natural sciencesHigh-performance liquid chromatographyAnalytical ChemistryAbsorbancechemistry.chemical_compoundColumn chromatographyDissolved organic carbonEnvironmental ChemistrytryptophanUV-254DOMWaste Management and Disposalta2180105 earth and related environmental sciencesWater Science and TechnologyChromatographygreywaterElution010401 analytical chemistryPublic Health Environmental and Occupational HealthPollutionproteinshumic0104 chemical scienceschemistryWastewaterIonic strengthfluorescenceionic strengthSodium acetateInternational Journal of Environmental Analytical Chemistry
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