HPLC-SEC: a new approach to characterise complex wastewater effluents

6533b860fe1ef96bd12c3b23

RESEARCH PRODUCT

HPLC-SEC: a new approach to characterise complex wastewater effluents

Hilda Marta Szabo Tuula Tuhkanen Raghida Lepistö

subject

Health Toxicology and Mutagenesis ta1172 Soil Science 010501 environmental sciences 01 natural sciences High-performance liquid chromatography Analytical Chemistry Absorbance chemistry.chemical_compound Column chromatography Dissolved organic carbon Environmental Chemistry tryptophan UV-254 DOM Waste Management and Disposal ta218 0105 earth and related environmental sciences Water Science and Technology Chromatography greywater Elution 010401 analytical chemistry Public Health Environmental and Occupational Health Pollution proteins humic 0104 chemical sciences chemistry Wastewater Ionic strength fluorescence ionic strength Sodium acetate

description

ABSTRACTThis work investigates the use of HPLC-SEC to characterise dissolved organic matter (DOM) of complex wastewater effluents. A silica-based column, sodium acetate eluent and multiple detections were employed: UV-254 absorbance for humictype, and tryptophan-like (Ex/Em = 270/355) and tyrosine-like (Ex/Em = 270/310) fluorescence for protein type compounds. Effects of eluent pH, eluent ionic strength and injection volume on separation efficiency were tested. Humic-type and protein-type fractions were clearly differentiated and eluted within and out of calibration range. Eluent ionic strength had the greatest influence on global resolution; the lowest eluent concentration of 0.01 M produced the best separation for all wastewater effluents tested at any detection. UV-254 absorbance was higher at neutral and basic eluent pH while tryptophan-like fluorescence depended on the sample composition rather than on the eluent pH or ionic strength. Tyrosine-like fluorescence decreased significantly with the increa...

year	journal	country	edition	language
2016-02-19	International Journal of Environmental Analytical Chemistry

https://doi.org/10.1080/03067319.2016.1150463

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