0000000000616065
AUTHOR
Hilda Marta Szabo
UV/VIS spectroscopy as a method to characterize well water quality. [UV/VIS-spektroskopi som metod att karakterisera brunnsvattenkvalité]
The applicability of UV/VIS spectroscopy for the characterization of well water quality was studied by analyzing 219 private wells from sparsely populated areas of Finland. In addition to conventional water quality indicators, such as conductivity, nitrate and DOC, a series of UV/VIS spectroscopic parameters were determined: A-224, A-254, A-280, A-365, A-436, the area under 250–350 nm, the area under 250–500 nm, SUVA-254, Molar Absorbance-280, Specific Color, A2/A3 and A2/A4, in order to find the most proper surrogate for the organic matter amount and the best indicator of organic matter quality. Three parameters, A-254, A-280 and the area under 250–350 nm were found to be applicable as sur…
HPLC-SEC: a new approach to characterise complex wastewater effluents
ABSTRACTThis work investigates the use of HPLC-SEC to characterise dissolved organic matter (DOM) of complex wastewater effluents. A silica-based column, sodium acetate eluent and multiple detections were employed: UV-254 absorbance for humictype, and tryptophan-like (Ex/Em = 270/355) and tyrosine-like (Ex/Em = 270/310) fluorescence for protein type compounds. Effects of eluent pH, eluent ionic strength and injection volume on separation efficiency were tested. Humic-type and protein-type fractions were clearly differentiated and eluted within and out of calibration range. Eluent ionic strength had the greatest influence on global resolution; the lowest eluent concentration of 0.01 M produc…