Search results for "Unity"

showing 10 items of 3852 documents

mRNA as a versatile tool for exogenous protein expression.

2012

Several viral and non-viral vectors have been developed for exogenous protein expression in specific cells. Conventionally, this purpose is achieved through the use of recombinant DNA. But mainly due to the risks associated with permanent genetic alteration of cells, safety and ethical concerns have been raised for the use of DNA-based vectors in human clinical therapy. In the last years, synthetic messenger RNA has emerged as powerful tool to deliver genetic information. RNA vectors exhibit several advantages compared to DNA and are particularly interesting for applications that require transient gene expression. RNA stability and translation efficiency can be increased by cis-acting struc…

RNA StabilityGenetic VectorsGene ExpressionComputational biologyBiologySmall hairpin RNADrug DiscoveryGene expressionGeneticsAnimalsHumansVector (molecular biology)RNA MessengerMolecular BiologyPost-transcriptional regulationGenetics (clinical)GeneticsMessenger RNAGene Transfer TechniquesRNAGenetic TherapyImmunity InnateRNA silencingRegulatory sequenceMolecular MedicineProtein Processing Post-TranslationalCurrent gene therapy
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Abscisic Acid Connects Phytohormone Signaling with RNA Metabolic Pathways and Promotes an Antiviral Response that Is Evaded by a Self-Controlled RNA …

2020

© 2020 The Authors.

RNA StabilityvirusesPotyvirusArabidopsisPlant Scienceantiviral immune evasionBiochemistryArticleTranscriptomeAbscisic acidPlant Growth RegulatorsPlant virusTobaccoPlant ImmunityMolecular BiologyImmune EvasionPlant DiseasesRNA metabolismbiologyfungimathematical modelingPotyvirusfood and beveragesRNARNA virusTranslation (biology)viral polyprotein processingCell BiologyViral polyprotein processingbiology.organism_classificationCell biologyViral replicationRNA PlantAntiviral immune evasionMathematical modelingMetabolic Networks and PathwaysAbscisic AcidSignal TransductionBiotechnologyPlant Communications
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Protection against myxomatosis and rabbit viral hemorrhagic disease with recombinant myxoma viruses expressing rabbit hemorrhagic disease virus capsi…

1996

Two myxoma virus-rabbit hemorrhagic disease virus (RHDV) recombinant viruses were constructed with the SG33 strain of myxoma virus to protect rabbits against myxomatosis and rabbit viral hemorrhagic disease. These recombinant viruses expressed the RHDV capsid protein (VP60). The recombinant protein, which is 60 kDa in size, was antigenic, as revealed by its reaction in immunoprecipitation with antibodies raised against RHDV. Both recombinant viruses induced high levels of RHDV- and myxoma virus-specific antibodies in rabbits after immunization. Inoculations by the intradermal route protected animals against virulent RHDV and myxoma virus challenges.

RNA virusesHemorrhagic Disease Virus Rabbitanimal diseasesvirusesLeporipoxviruslaw.invention0403 veterinary sciencelawVIRUS RECOMBINEComputingMilieux_MISCELLANEOUSCaliciviridae Infections[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology0303 health sciencesVaccines SyntheticMyxomatosisLagovirus04 agricultural and veterinary sciences3. Good healthLagovirusCapsid[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/VirologyRecombinant DNAcardiovascular systemRabbitsReassortant VirusesLeporipoxvirusResearch Article040301 veterinary sciencesImmunologyDNA RecombinantMyxoma virusBiologyMicrobiologyVirus03 medical and health sciencesMyxomatosis InfectiousImmunity reactionsVirologyReassortant VirusesmedicineAnimalsRabbit diseasesRecombinant vaccinescardiovascular diseasesImmune response030304 developmental biologyViral Structural ProteinsViral infectionsMyxoma virusbiology.organism_classificationmedicine.diseaseVirologyMédecine vétérinaire et santé animalInsect ScienceExperimental infectionsDNA ViralDNA virusesJournal of virology
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Equal Opportunities Analysis in the University: The Gender Perspective

2003

The social systems’ complexity is a consequence of the human presence. Measurements and evaluations are rather qualitative and, in many cases, heuristic and / or linguistic. Human beings are not equally treated, with a clear discrimination based on age, gender, race or culture, among many other reasons. Women discrimination is the focus of our study in this paper. The university, as a social system, is investigated. The goal is to determine the degree of gender discrimination and to provide the tools to evaluate different actions to improve the equal opportunity (EO) principle in its operation.

Race (biology)Gender discriminationComputer scienceSocial systemPerspective (graphical)Social psychologyEqual opportunityFocus (linguistics)
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La transmissió lingüística intergeneracional a Castelló de la Plana i la Franja: La rellevància dels usos entre progenitors

2020

Fa 50 anys, a Conflicte lingüístic valencià, Rafael L. Ninyoles situava el trencament de la transmissió familiar del català com a resultat i manifestació externa de conflictes socials i de classes, especialment visibles enmig del procés de modernització econòmica i mobilitat social accelerada del País Valencià dels anys 60 del segle XX. En aquest article partim de dos estudis de cas quantitatius sobre la transmissió intergeneracional de les llengües a Castelló de la Plana i les comarques centrals de la Franja per a actualitzar el seu diagnòstic i matisar la visió d'aquest fenomen com a resultat de decisions dels progenitors imposades verticalment a la descendència. Hi proposem que els usos …

Rafael L. Ninyoles situava el trencament de la transmissió familiar del català com a resultat i manifestació externa de conflictes socials i de classesNacionalismeintergenerational transmission 265 284BilingualismSorolla1137-7038 8537 Arxius de sociologia 562372 2020 42 7674046 La transmissió lingüística intergeneracional a Castelló de la Plana i la Franja: La rellevància dels usos entre progenitors Flors i MasNatxo Fa 50 anysLa rellevància dels usos entre progenitors Flors i Mas [1137-7038 8537 Arxius de sociologia 562372 2020 42 7674046 La transmissió lingüística intergeneracional a Castelló de la Plana i la Franja]Castelló de la Plana (País Valencià)Segle XXCatalàAdquisició del llenguatgeUNESCO::SOCIOLOGÍACastellón de la Plana (Valencian Community)Language attritionBilingüismeCatalan languageNationalismlanguageFranja de Ponent (Aragó)20th centuryAve?líLanguage acquisition:SOCIOLOGÍA [UNESCO]Native languagea Conflicte lingüístic valenciàFranja de Ponent (Aragon)Communication in the familyLlengua maternaComunicació en la famíliaPèrdua de les llengüesespecialment visibles enmig del procés de modernització econòmica i mobilitat social accelerada del País Valencià dels anys 60 del segle XX. En aquest article partim de dos estudis de cas quantitatius sobre la transmissió intergeneracional de les llengües a Castelló de la Plana i les comarques centrals de la Franja per a actualitzar el seu diagnòstic i matisar la visió d?aquest fenomen com a resultat de decisions dels progenitors imposades verticalment a la descendència. Hi proposem que els usos lingüístics intrageneracionals entre progenitors són un factor explicatiu clau de la interrupció de la transmissió Catalan
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New, Rare and Constant Habitats for Endangered Aquatic Plant Communities: The Importance of Microhabitats for Global biodiversity

2019

Natural water reservoirs are very valuable floristic sites, with springs particularly important for the preservation of floral biodiversity. This paper presents, as a case study, a community of water plants that is new to limnocrene karst springs in Europe: Potametum alpini (Potametea), found in Poland. The paper provides the floristic composition and ecological requirements of this plant association, which is rare and endangered in Europe. According to our knowledge, the habitat data presented here are unique as they are published for the first time for this plant community, and thus it is currently not possible to compare them with data from other authors. Our study confirms the importanc…

Range (biology)BiodiversityEndangered species01 natural sciencesglobal biodiversity; karst springs; microhabitats; rare habitats; rare plant communities03 medical and health sciencesAquatic plant010503 geologylcsh:QH301-705.50105 earth and related environmental sciencesNature and Landscape Conservation0303 health sciencesmicrohabitatsEcologyEcologyEcological Modeling030302 biochemistry & molecular biologykarst springsrare plant communitiesPlant communityAgricultural and Biological Sciences (miscellaneous)Water resourcesGeographyHabitatlcsh:Biology (General)rare habitatsglobal biodiversityGlobal biodiversityDiversity
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Helminth communities of owls (strigiformes) indicate strong biological and ecological differences from birds of prey (accipitriformes and falconiform…

2012

We compared the helminth communities of 5 owl species from Calabria (Italy) and evaluated the effect of phylogenetic and ecological factors on community structure. Two host taxonomic scales were considered, i.e., owl species, and owls vs. birds of prey. The latter scale was dealt with by comparing the data here obtained with that of birds of prey from the same locality and with those published previously on owls and birds of prey from Galicia (Spain). A total of 19 helminth taxa were found in owls from Calabria. Statistical comparison showed only marginal differences between scops owls (Otus scops) and little owls (Athene noctua) and tawny owls (Strix aluco). It would indicate that all owl …

Range (biology)ScienceVeterinary MicrobiologyZoologyOtus scopsBiologyGeneralist and specialist speciesMicrobiologyPredationbiology.animalHelminthsAnimalsCommunity AssemblyBiologyCommunity StructureFalconiformesPhylogenyMultidisciplinaryEcologyEcologyBird DiseasesQRSpecies diversitybiology.organism_classificationStrigiformesVeterinary ParasitologyStrix alucoStrigiformesItalyCommunity EcologyVeterinary DiseasesAccipitriformesMedicineParasitologyVeterinary ScienceHelminthiasis AnimalZoologyResearch ArticleHelminthologyPLoS ONE
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Indirect evaluation of pit and fissure sealants: a 3D-based method validation

2019

Background The aim of the present study was to compare indirect methods to assess the clinical performance of pit and fissure sealants and validate the use of 3D scanners. Material and Methods Sample consisted of 58 plaster models of upper and lower first permanent molars, sealed with resin sealants, as well as photographs obtained during the 18-month follow-up. Pre-established criteria were applied to categorize the sealant presence/absence and marginal integrity. Two calibrated examiners performed the evaluations, independently, using Scanning Electron Microscopy (SEM; gold-standard), Photography, 3D (CEREC In Lab) and Stereomicroscope analysis. Results The intra-examiner Spearman correla…

Receiver operating characteristicFissurebusiness.industrySealantResearchDentistryGold standard (test):CIENCIAS MÉDICAS [UNESCO]Spearman's rank correlation coefficientCommunity and Preventive DentistryFOTOGRAFIA EM ODONTOLOGIAmedicine.anatomical_structureCERECStereo microscopeUNESCO::CIENCIAS MÉDICASmedicinebusinessGeneral DentistryKappaMathematics
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Lipid presentation by the protein C receptor links coagulation with autoimmunity.

2021

A lipid-protein autoimmunity target Several autoimmune diseases, including systemic lupus erythematosus and primary antiphospholipid syndrome, are characterized by the presence of antiphospholipid antibodies (aPLs). These molecules can activate the complement and coagulation cascades, which contributes to pathologies such as thrombosis, stroke, and pregnancy complications. Müller-Calleja et al. found that endothelial protein C receptor (EPCR) in complex with lysobisphosphatidic acid (LBPA) is the cell-surface target for aPL and mediates its internalization (see the Perspective by Kaplan). aPL binding to EPCR-LBPA resulted in the activation of tissue factor–mediated coagulation and interfero…

Receptor complexAntigen presentationAutoimmunityEndosomesmedicine.disease_causeArticleAutoimmunityMiceInterferonimmune system diseasesmedicineAnimalsHumansLupus Erythematosus SystemicneoplasmsBlood CoagulationAutoantibodiesAutoimmune diseaseEndothelial protein C receptorAntigen PresentationMultidisciplinaryInnate immune systemLupus erythematosusEndothelial Protein C ReceptorThrombosismedicine.diseaseAntiphospholipid SyndromeImmunity InnateMice Mutant StrainsDisease Models AnimalSphingomyelin PhosphodiesteraseToll-Like Receptor 7ImmunologyAntibodies AntiphospholipidEmbryo LossMonoglyceridesEndothelium VascularLysophospholipidsmedicine.drugScience (New York, N.Y.)
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Amazing IL-9: revealing a new function for an “old” cytokine

2012

Th9 cells are a subset of CD4+ Th cells that produce the pleiotropic cytokine IL-9. IL-9/Th9 can function as both positive and negative regulators of immune response, but the role of IL-9/Th9 in tumor immunity is unknown. We examined the role of IL-9/Th9 in a model of pulmonary melanoma in mice. Lack of IL-9 enhanced tumor growth, while tumor-specific Th9 cell treatment promoted stronger antitumor responses in both prophylactic and therapeutic models. Th9 cells also elicited strong host antitumor CD8+ CTL responses by promoting Ccl20/Ccr6-dependent recruitment of DCs to the tumor tissues. Subsequent tumor antigen delivery to the draining LN resulted in CD8+ T cell priming. In agreement with…

Receptors CCR6Lung Neoplasmsmedicine.medical_treatmentBiologyCD8-Positive T-LymphocytesMiceImmunityCell Line TumormedicineAnimalsLack of knowledgeInterleukin 9MelanomaMice KnockoutAntigen PresentationImmunity CellularChemokine CCL20Antitumor immunityMelanomaInterleukin-9General MedicineDendritic CellsT-Lymphocytes Helper-Inducermedicine.diseaseCytokineImmunologyCommentaryImmunotherapySkin cancerFunction (biology)
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