Search results for "VECTOR"
showing 10 items of 2660 documents
Electrocardiographic Imaging Using a Spatio-Temporal Basis of Body Surface Potentials—Application to Atrial Ectopic Activity
2018
Electrocardiographic imaging (ECGI) strongly relies on a priori assumptions and additional information to overcome ill-posedness. The major challenge of obtaining good reconstructions consists in finding ways to add information that effectively restricts the solution space without violating properties of the sought solution. In this work, we attempt to address this problem by constructing a spatio-temporal basis of body surface potentials (BSP) from simulations of many focal excitations. Measured BSPs are projected onto this basis and reconstructions are expressed as linear combinations of corresponding transmembrane voltage (TMV) basis vectors. The novel method was applied to simulations o…
Reductive modification of genetically encoded 3-nitrotyrosine sites in alpha synuclein expressed in E.coli
2019
Tyrosine nitration is a post-translational protein modification relevant to various pathophysiological processes. Chemical nitration procedures have been used to generate and study nitrated proteins, but these methods regularly lead to modifications at other amino acid residues. A novel strategy employs a genetic code modification that allows incorporation of 3-nitrotyrosine (3-NT) during ribosomal protein synthesis to generate a recombinant protein with defined 3-NT-sites, in the absence of other post-translational modifications. This approach was applied to study the generation and stability of the 3-NT moiety in recombinant proteins produced in E.coli. Nitrated alpha-synuclein (ASYN) was…
On the limit velocity and buckling phenomena of axially moving orthotropic membranes and plates
2011
In this paper, we consider the static stability problems of axially moving orthotropic membranes and plates. The study is motivated by paper production processes, as paper has a fiber structure which can be described as orthotropic on the macroscopic level. The moving web is modeled as an axially moving orthotropic plate. The original dynamic plate problem is reduced to a two-dimensional spectral problem for static stability analysis, and solved using analytical techniques. As a result, the minimal eigenvalue and the corresponding buckling mode are found. It is observed that the buckling mode has a shape localized in the regions close to the free boundaries. The localization effect is demon…
Multicompartmental Lipopolyplex as Vehicle for Antigens and Genes Delivery in Vaccine Formulations
2021
Vector design and its characterization is an area of great interest in current vaccine research. In this article, we have formulated and characterized a multicompartmental lipopolyplex, which associates multiple liposomes and polyplexes in the same complex. These particles allow the simultaneous delivery of lipid or water-soluble antigens associated with genes to the same cell, in much higher amounts than conventional lipopolyplexes. The vector characterization and optimization were carried out using liposomes with entrapped carboxyfluorescein and adapted electrophoretic assays. Two types of lipopolyplexes (containing hydrophilic or lipophilic antigens) were employed to evaluate their inter…
Evaluation of the efficacy of a new insecticide paint against malaria vectors
2015
La malaria continúa siendo un problema de salud pública global. Se calcula que en el mundo hay 3.300 millones de personas en 97 países y territorios que corren el riesgo de padecer el paludismo, y que para 1.200 millones ese riesgo es elevado (WHO, 2014). El control de la malaria a gran escala está altamente basado en al control del vector, principalmente mediante el uso de mosquiteras tratadas con insecticidas de larga duración (LLINs), y en menor medida, el Rociamiento Residual Intradomiciliario. Todos los tratamientos recomendados de LLINs y la mayoría del Rociamiento Residual Intradomiciliario se basan en el uso de piretroides. La creciente resistencia de los vectores de malaria a los p…
Systematic Study of a Library of PDMAEMA-Based, Superparamagnetic Nano-Stars for the Transfection of CHO-K1 Cells.
2017
The introduction of the DNA into mammalian cells remains a challenge in gene delivery, particularly in vivo. Viral vectors are unmatched in their efficiency for gene delivery, but may trigger immune responses and cause severe side-reactions. Non-viral vectors are much less efficient. Recently, our group has suggested that a star-shaped structure improves and even transforms the gene delivery capability of synthetic polycations. In this contribution, this effect was systematically studied using a library of highly homogeneous, paramagnetic nano-star polycations with varied arm lengths and grafting densities. Gene delivery was conducted in CHO-K1 cells, using a plasmid encoding a green fluore…
Assessing the Commodity Market Price and Terms of Trade Exposures of Macroeconomy in Emerging and Developing Countries
2021
This paper provides novel evidence on commodity market exposure, i.e., the impacts of commodity price and terms of trade fluctuations on macro performance amongst 46 emerging and developing countries (EMDCs) in Africa, Asia and the Latin American and Caribbean (LAC) region. We estimate the exposure of six macroeconomic variables to the commodity prices and terms of trade. Our results indicate that in overall terms, there is a strong and statistically significant long-run relationship between the vector of analyzed world trade prices and macro variables in all EMDCs. However, based on the short-term reactions, only about 10% of the macroeconomic variation amongst the EMDCs is due to commodit…
A blow-up result for a nonlinear wave equation on manifolds: the critical case
2021
We consider a inhomogeneous semilinear wave equation on a noncompact complete Riemannian manifold (Formula presented.) of dimension (Formula presented.), without boundary. The reaction exhibits the combined effects of a critical term and of a forcing term. Using a rescaled test function argument together with appropriate estimates, we show that the equation admits no global solution. Moreover, in the special case when (Formula presented.), our result improves the existing literature. Namely, our main result is valid without assuming that the initial values are compactly supported.
Geometric Aspects of Thermodynamics
2016
This chapter deals with mathematical aspects of thermodynamics most of which will be seen to be primarily of geometrical nature. Starting with a short excursion to differentiable manifolds we summarize the properties of functions, of vector fields and of one-forms on thermodynamic manifolds. This summary centers on exterior forms over Euclidean spaces and the corresponding differential calculus. In particular, one-forms provide useful tools for the analysis of thermodynamics. A theorem by Caratheodory is developed which is closely related to the second law of thermodynamics. The chapter closes with a discussion of systems which depend on two variables and for which there is an interesting a…
Fundamental bounds on qubit reset
2020
Qubit reset is a basic prerequisite for operating quantum devices, requiring the export of entropy. The fastest and most accurate way to reset a qubit is obtained by coupling the qubit to an ancilla on demand. Here, we derive fundamental bounds on qubit reset in terms of maximum fidelity and minimum time, assuming control over the qubit and no control over the ancilla. Using the Cartan decomposition of the Lie algebra of qubit plus two-level ancilla, we identify the types of interaction and controls for which the qubit can be purified. For these configurations, we show that a time-optimal protocol consists of purity exchange between qubit and ancilla brought into resonance, where the maximu…