Search results for "VITRO"

Human histidine-rich glycoprotein expressed in SF9 insect cells inhibits apatite formation

1997

Histidine-rich glycoprotein (HRG) is structurally related to the alpha2-HS glycoprotein/fetuin family of mammalian plasma proteins; both belong to the cystatin superfamily of proteins. We expressed recombinant human HRG and alpha2-HS in Sf9 insect cells for functional analysis. Recombinant HRG bound heparin and fibrinogen while alpha2-HS did not. Both proteins inhibited the formation of apatite, recombinant HRG (IC50 approximately 1 microM) with 2-fold lower molar activity than alpha2-HS (IC50 approximately 0.5 microM). The inhibition in vitro of apatite formation suggests a new function for plasma HRG protein, inhibition of phase separation in blood vessels.

Injectable Hydrogel for Synergetic Low Dose Radiotherapy, Chemodynamic Therapy and Photothermal Therapy

2021

Higher doses of radiotherapy (RT) are associated with resistance induction, therefore highly selective and controllable radiosensitizers are urgently needed. To address this issue, we developed a FeGA-based injectable hydrogel system (FH) that can be used in combination with low-dose radiation. Our FH can deliver FeGA directly to the tumor site via intratumoral injection, where it is a reservoir-based system to conserve FeGA. The photothermal properties of FeGA steadily dissolve FH under laser irradiation, and, simultaneously, FeGA reacts with a large amount of H2O2 in the cell to produce OH (Fenton reaction) which is highly toxic to mitochondria, rendering the cell inactive and reducing ra…

Isoproterenol inhibits fluid-phase endocytosis from early to late endosomes

1999

We have shown recently that isoproterenol affects both the cellular location and the morphology of late endosomes in a pH-dependent manner [Marjomäki et al., Eur. J. Cell Biol. 65, 1-13 (1994)]. In this study, using fluorescence and quantitative electron microscopy, we wanted to examine further what is the fate of internalized markers during their translocation from early to late endosomes under isoproterenol treatment. Fluorescein dextran internalized for 30 min (10-min pulse followed by a 20-min chase) showed accumulation in the cellular periphery during isoproterenol treatment in contrast to the control cells, which accumulated dextran in the perinuclear region. Quantitative electron mic…

Influence of Different Bisphosphonates on Keratinocytes, HUVEC, Fibroblasts and Osteoblasts in-vitro

2010

Activation of Human Osteoblasts via Different Bovine Bone Substitute Materials With and Without Injectable Platelet Rich Fibrin in vitro

2021

IntroductionThe aim of the in vitro study was to compare the effect of four bovine bone substitute materials (XBSM) with and without injectable platelet-reach fibrin for viability and metabolic activity of human osteoblasts (HOB) as well as expression of alkaline phosphatase (ALP), bone morphogenetic protein 2 (BMP-2), and osteonectin (OCN).Materials and MethodsCerabone® (CB), Bio-Oss® (BO), Creos Xenogain® (CX) and MinerOss® X (MO) ± i-PRF were incubated with HOB. At day 3, 7, and 10, cell viability and metabolic activity as well as expression of ALP, OCN, and BMP-2, was examined.ResultsFor non-i-PRF groups, the highest values concerning viability were seen for CB at all time points. Pre-t…

A heterocyclic oxime from a fungus with anti-juvenile hormone activity

1998

Two fractions obtained after chromatography of dichloromethane extract of Penicillium brevicompactum culture medium showed anti-juvenile hormone activity. One was active when assayed in vivo against Oncopeltus fasciatus third-instar nymphs, whereas the other showed a strong in vitro inhibition of JH III biosynthesis on Locusta migratoria corpora allata. A subfraction of the latter, constituting 97% of this fraction, is a main component that possessed the juvenile hormone biosynthesis inhibitory activity. Chemical characterization of this compound shows a sesquiterpene-like structure, molecular mass of 278.16185 daltons, corresponding to an empirical formula of C15H22N2O3 named brevioxime. E…

OCCURRENCE OF SPONTANEOUS TRIPLOIDS FROM SMALL SEEDS OF 'HERNANDINA' CLEMENTINE

2011

Micropropagation ofAgeratum houstonianumby nodal segments

2017

Ageratum houstonianum is a bedding and flowering potted plant originated from Central America which is generally propagate by seed. In this report a preliminary in vitro technique for propagation of A. houstonianum was investigated. In vitro germinated seeds were used to establish aseptic shoot cultures of several clones. Seedling stem segments bearing 3-4 nodes were placed on Murashige and Skoog (MS) basal medium plus 20 g L-1 sucrose, 8.0 g L-1 Agar to induce axillary shoot development. Axillary shoots were subcultured into the same medium and nodal segments were sectioned and subcultured to increase the stock of shoot cultures. Shoot cultures of the selected clone AG14 were used to accom…

Regulation of Rooting Ability of Solanum tuberosum L. Explants by Gaseous Environment, In Vitro

1997

It has been reported by many investigators that morphogenesis of expiant tissue can be regulated by the composition of the nutrient medium as well as by the gaseous environment (Buddendorf-Joosten and Woltering, 1994). The aim of the present study was to investigate different effects on rooting ability of potato expiants in vitro, caused by limited gas exchange and IBA and BAP treatment.

EX VITRO MYCORRHIZATION OF VITRO-DERIVED PLANTLETS OF 'CARRIZO' CITRANGE [C. SINENSIS (L.) OSB. × P. TRIFOLIATA (L.) RAF.]

2015

In this study, vitro-derived plantlets of ‘Carrizo’ citrange were inoculated with Glomus intraradices during the acclimatization phase, from in vitro to in vivo. Plantlets were grown in presence (+M) and in absence of inoculum (-M) and in addition, in sterile and non-sterile substrate. After three months of culture, some vegetative parameters and the level of infection, varied greatly in the different theses. In particular, the highest formation of new branched roots has been observed in inoculated plantlets cultivated in sterile substrate. Good results were observed also in non-inoculated plantlets cultivated in non-sterile substrate, in presence of endemic inoculum. The same trend was…