Search results for "VITRO"

Chemical Proteomics-Guided Identification of a Novel Biological Target of the Bioactive Neolignan Magnolol

2019

Understanding the recognition process between bioactive natural products and their specific cellular receptors is of key importance in the drug discovery process. In this outline, some potential targets of Magnolol, a natural bioactive compound, have been identified by proteomic approaches. Among them, Importin-β1 has been considered as the most relevant one. A direct binding between Magnolol and this nuclear chaperone has been confirmed by DARTS and molecular docking, while its influence on Importin-β1 translocation has been evaluated by in vitro assays.

Antagonism and Antimicrobial Capacity of Epiphytic and Endophytic Bacteria against the Phytopathogen Xylella fastidiosa

2022

Olive quick decline syndrome (OQDS), which is caused by Xylella fastidiosa, poses a severe threat to the agriculture of Mediterranean countries and causes severe damage to the olive trees in Italy. Since no effective control measures are currently available, the objective of this study was the screening of antagonistic bacteria that are potentially deployable as biocontrol agents against X. fastidiosa. Therefore, two approaches were used, i.e., the evaluation of the antagonistic activity of (i) endophytic bacteria isolated from two different cultivars of olive trees (Leccino and Ogliarola salentina) and (ii) epiphytic bacteria isolated from the phyllospheres of different host plant species …

Wybrane problemy bioetyczne w rozumieniu Kościołów pentekostalnych i Kościoła katolickiego. Studium teologiczno-porównawcze

2018

Rozwój nauk medycznych stwarza potrzebę oceny, które z nowych odkryć służą rzeczywiście dobru człowieka, a które są zagrożeniem dla jego egzystencji, godności i nienaruszalności. Przygnębiającym jest fakt, iż wyznawcy Chrystusa nie tylko są podzieleni w kwestiach wielu artykułów wiary, ale także mają odmienne nauczanie dotyczące norm etycznych. Niespodziewanie nauka moralności pentekostalnej oraz katolickiej w problematyce aborcji, zapłodnienia pozaustrojowego in vitro i eutanazji w wielu punktach okazuje się zbieżna. Obie tradycje stoją na stanowisku, że życie ludzkie zaczyna się w momencie zapłodnienia i ma szczególną wartość, aborcja jest złem i niedopuszczalne jest stosowanie praktyk ma…

Cytotoxic eudesmane sesquiterpenes from Crepis sancta

2019

Abstract Chemical exploration of Crepis sancta (L.) Bornm. (Asteraceae) aerial parts afforded two new eudesmane sesquiterpenes (1 and 2) together with three known congeners (3-5) and two known methylated flavonoids (6 and 7). Structure elucidation of the new compounds was unambiguously performed based on HRESIMS, 1D and 2D NMR spectroscopic analyses. All isolated compounds were subjected to an in vitro cytotoxicity assay against mouse lymphoma (L5178Y) cells, revealing moderate activities with IC50 ranging from 7.9 to 21.0 μM.

Ploidy manipulation for genetic improvement in some Mediterranean fruit crops

2017

Plant breeding is focused on selection of new genotypes with improved traits. Conventional methods based on hybridization and those based on biotechnology (somatic hybridization, genetic transformation, ploidy manipulation, etc.) are used to create novel genetic variations. Biotechnology provides powerful tools for plant breeding, for instance, haploid technology allows achievement of homozygous lines from heterozygous parents in one step, which reduces significantly the time required by conventional methods. Concerning woody species, characterized by self-incompatibility, long juvenile period and high degree of heterozygosity, this technique is the only way to get homozygous lines. Haploid…

In vitro efficacy of praziquantel against the cercariae of Diplostomum sp., Rhipidocotyle fennica and R. campanula.

2009

Relationship between HLA I surface expression and different cytopathic effects produced after herpes simplex virus infection in vitro.

1992

In the present study, we investigated the effects of herpes simplex virus (HSV) infection on the expression of HLA class I antigens and beta 2-microglobulin in human fibroblasts. The mRNA abundance for HLA class I was shown to be strongly reduced after infection with HSV strains either producing cell rounding or fusion from within (FFWI), however, HLA class I expression on the surface of cells is strongly reduced only after appearance of FFWI. Using a ts mutant (ts 78R) or CyA in combination with a fusion from without (FFWO) inducing strain of HSV, this loss of HLA class I antigens is assumed to be correlated to the rearrangement of the cell membrane during the fusion process itself as a la…

A comparative study of the in vitro immunomodulatory activity of human intact immunoglobulin (7S IVIG), F(ab′) 2 fragments (5S IVIG) and Fc fragments…

1997

During the past few decades intravenous immunoglobulin (IVIG) has been used successfully in the treatment of various immunoregulatory disorders. Treatment results have been attributed to immunomodulation mainly via Fc receptors or by anti-idiotypic antibodies to disease-causing autoantibodies. From the present study it is clearly evident that 7S IVIG (intact immunoglobulin) as well as 5S IVIG [F(ab')2 fragments] and Fc fragments have a potent immunomodulatory capacity. We demonstrate that mainly 7S IVIG inhibits alloantigen-induced T-cell proliferation and generation of cytotoxic T lymphocytes. Reduced interleukin-2 (IL-2) protein levels in culture supernatants of IVIG-supplemented mixed ly…

Micropropagation of Genista aetnensis [(Raf. ex Biv.)DC]

2015

Genista aetnensis [(Raf. ex Biv.)DC] is a large deciduous shrub or small tree native to the Italian islands of Sardinia and Sicily. Being winter hardy and characterized by high plasticity in altitude and ecology, the species is grown in gardens and landscaping, both for flower and for its attractive shape. Genista species are generally propagate by seed or semi hardwood cuttings. In this report an efficient in vitro technique for propagation of G. aetnensis was investigated. Multiple shoots were induced on nodal segments of a mature plant of Genista aetnensis . The Murashige and Skoog medium, augmented with different concentrations of benzyladenine either singly or in combination with indol…

Induction of (2′−5′)oligoadenylate synthetase in the marine spongesSuberites domunculaandGeodia cydoniumby the bacterial endotoxin lipopolysaccharide

2002

Recent studies have shown that the Porifera, with the examples of the demosponges Suberites domuncula and Geodia cydonium, comprise a series of pathways found also in the immune system of Deuterostomia, such as vertebrates, but are absent in Protostomia, with insects or nematodes as examples. One pathway is the (2'-5')oligoadenylate synthetase [(2-5)A synthetase] system. In the present study we show that crude extracts from tissue of S. domuncula collected from the sea display a considerable amount of (2-5)A synthetase activity; 16% of the ATP substrate is converted to the (2-5)A product, while tissue from specimens which were kept for 6 months in an aquarium shows only 1% of conversion. As…