Search results for "VITRO"

Organometallic complexes with biological molecules: XIII. Organotin(IV)[meso-tetra (4-carboxyphenyl)porphinate]s and the cell cycle: a flow-cytometri…

1999

The cytotoxic derivatives diorganotin(IV) and triorganotin(IV) [meso-tetra(4-carboxyphenyl)porphinates, with stoichiometries [R2Sn]2TPPC and [R3Sn]4TPPC [R = Me, Bu, Ph; TPPC4−­= meso-tetra(4-carboxyphenyl)porphinate4−], namely bis[dimethyltin(IV)], bis[dibutyltin(IV)], bis[diphenyltin(IV)], tetra[trimethyltin(IV)], tetra[tributyltin(IV)] and tetra[triphenyltin(IV)] [meso-tetra(4-carboxyphenyl)porphinate]s, have been used to investigate their effects on the cultured human kidney cell cycle in order to understand further the origin of cell-growth inhibition induced by the above-mentioned chemicals. The cell-cycle-dependent DNA content distribution of cultured cells exposed to these compounds…

TRANSPLANTATION AND IN VITRO ANALYSIS OF SOYBEAN-AGGLUTININ SEPARATED MOUSE SPLEEN CELLS

1984

In human bone marrow allotransplantation, an in vitro method using soybean agglutinin(SBA) for enrichment of stem cells and depletion of mature T cells in the graft has been described (Reisner et al.;Lancet 2:1320,1980).To define the quality of the method,mouse spleen cells (C3H, C57/B6,BALB/c)containing about 30% of mature T cells were separated by SBA.The composition and functional capacity of the cell fraction known to contain the stem cells were characterized in vitro after each of two SBA-separation steps.In addition, the ability of the cell fraction to reconstitute successfully allogeneic irradiated (900R) mice was investigated. Only the two step SBA-separation procedure yielded satis…

Effect of poly(I).poly(C12U) (Ampligen) on enteric virus (rotavirus, poliovirus and Coxsackie B3 virus) infections

1994

The effects of poly(1)-poly(C 12 U) (Ampligen) on infections with enteric viruses (rotavirus, poliovirus and Coxsackie B3 virus) were studied in vitro. Ampligen exhibited antiviral activity against rotavirus, especially when treatment was performed prior to inoculation of the virus. It was partially effective against Coxsackie B3 virus, but not against poliovirus. It is suggested that the observed effects may be due to the production of interferon induced by Ampligen

New glucocorticoids. Mechanisms of immunological activity at the cellular level and in the clinical setting.

1990

RESTORATION OF LATVIAN ALFALFA (Medicago sativa) GENETIC RESOURCES PERSPECTIVE FOR BREEDING

2015

We have carried out restoring of some Latvian alfalfa genetic resources from seeds of accessions that did not germinate in soil at all. Two of them were repatriated from the N. Vavilov All-Russian Institute of Plant Industry (multiplied more than 40 years ago) and three from the Research Institute of Agriculture (Latvia) (multiplied 15-20 years ago). For germination of old seeds we used early elaborated in vitro culture conditions. Germination rate ranged 2-60%, depending from the genotype and seeds storage conditions. Plantlets with well developed roots and 2-3 leaves were planted in the substrate in small pots and grown in a greenhouse about a month, then replanted in the soil in field co…

Comparison of the shaping characteristics of Neolix and Protaper Universal systems in preparation of severely-curved simulated canals

2017

Background The aim of this in vitro study was to compare the shaping characteristics of two types of nickel-titanium endodontic file systems with regard to the following parameters: Canal straightening, Canal deviation and Instrumentation time. Material and Methods Fifty severely curved canals simulated in resin blocks were prepared to an apical size 25 using Protaper Universal orNeolix systems (n=25 canals/group). The angle of canal curvature was determined before and after instrumentation. Pre- and post-operative images were superimposed to determine any canal deviation. The instrumentation time was also recorded. The data were statistically analyzed by using independent sample t-test and…

Tooth discoloration and internal bleaching after the use of ledermix paste with various bleaching agents ? An in vitro study

2018

Background To assess the reversal of discolouration caused by Ledermix using various bleaching agents. Material and Methods Twenty six extracted human mandibular premolars were taken and divided into four groups. Six teeth were divided into three each which are taken as positive and negative controls. The remaining twenty are divided into two groups (n=10). After conventional access preparation, the Ledermix paste was sealed in the pulp chamber for twelve weeks. The paste was removed by a rinse with sodium hypochlorite (NaOCl). Then the pulp chamber was sealed with a mixture of Sodium perborate and distilled water for group 1 and group 2 was sealed with Sodium tetraborate for 1 to 12 weeks.…

Influence of Different Rehydration Protocols on Biomechanical Properties of Allogeneic Cortical Bone Plates: A Combined in-vitro/in-vivo Study

2020

Introduction: Allogeneic cortical bone plates (CP) are used for alveolar ridge augmentation. Since CP are freeze-dried and dehydrated during processing, the breaking strength (BS) and the flexibility (FX) are reduced, resulting in a relevant risk for plate fractures during insertion. The aim of this study was to evaluate the influence of rehydration time on the biomechanical properties (BS & FX) of CP in-vitro and in vivo.Material and Methods: 40 CP were randomly divided into four experimental groups. (A) untreated control (n = 10), rehydration for 10 (B), 30 (C) and 60 (D) minutes in 0.9% saline solution (n = 10 each). BS [Newton, N] and FX [mm] (force till fracture and distance of deflect…

Combining conventional chemotherapy and γδ T cell-based immunotherapy to target cancer-initiating cells.

2013

According to common beliefs, conventional anticancer chemotherapy is deleterious for the immune system. We have recently provided in vitro evidence indicating that conventional chemotherapy may potentiate, rather than impair, the long-term efficacy of γδ T cell-based anticancer immunotherapy.

The caramel flavour: impacts of the volatile and the non-volatile fraction on perception

2013

The aim of this thesis was to investigate the impact of the volatile odorant fraction and the non-volatile fraction on the perception of caramel flavour. The first step focused on the analytical characterization of the volatile odorant fraction of caramel using Gas Chromatography coupled to Olfactometry (GC/O), GC/Mass Spectrometry (GC/MS) and heart-cutting MDGC/MS/O. Results highlighted the presence of more than one hundred compounds and demonstrated the complexity of the caramel volatile fraction. The objective of the second step was to understand the contribution of odorant compounds identified to the caramel aroma typicality. Literature shows that odour intensity is not sufficient to ev…