Search results for "VITRO"
showing 10 items of 2786 documents
Elevated cortisol modulates hsp70 and hsp90 gene expression and protein in sea bass head kidney and isolated leukocytes
2012
In fish, interactions between Hsps and cortisol are involved in stress modulated physiological processes including innate immune responses. Cortisol exerts a role in the regulation of Hsps synthesis. Fish head kidney is a lymphomieloid and endocrine organ releasing cortisol, and it is the central organ for immune-endocrine interactions. In sea bass, cortisol intraperitoneal injection and in vitro treatment of head kidney cells show that inducible Hsp70 and Hsp90 are modulated by this hormone. However, an inverse relationship between mRNA expression (real-time PCR) and Hsp70 and Hsp90 protein levels (densitometric band analysis) was found. Time-course assays indicate a cortisol-mediated regu…
Humic acid reduces gonadotropin activity and hormonal sensitivity of frog oocytes
2005
The specific stimulatory effect of sturgeon Acipenser güldenstädti Br. gonadotropic hormone (GTH) on frog Rana temporaria L. oocyte maturation in vitro was investigated in relation to humic acid (HA) concentrations from 12.5 to 50 mg/l. HA was observed to bind to both the follicular membrane of the oocytes and the GTH molecule, reducing the oocytes' hormone sensitivity and maturation ability. It was also shown that HA inactivated GTH, lowering its specific ability to stimulate oocyte maturation.
Mecanismos de acción citotóxica producidos por la esterigmatocistina en las células sh-sy5y de neuroblastoma humano
2021
En la presente Tesis Doctoral se ha llevado a cabo la evaluación in vitro de los efectos producidos por la micotoxina esterigmatocistina (STE) en células de mamífero. El conocimiento de los efectos tóxicos y exposición a dicha micotoxina permite una mejor caracterización de los riesgos de la población. Se ha evaluado la citotoxicidad individual de la STE en células de neuroblastoma (SH-SY5Y) y de carcinoma hepatocelular humano (HepG2), mostrando valores de IC50 únicamente en las células SH-SY5Y. Debido a que la exposición simultánea o secuencial a múltiples micotoxinas podría dar lugar a efectos adversos sobre la salud del consumidor, se ha evaluado la citotoxicidad combinada de la STE con …
Potencial uso de la tecnología basada en microARNs para el diagnóstico y pronóstico de la escoliosis idiopática del adolescente
2021
Introducción: La escoliosis idiopática del adolescente (EIA) es una enfermedad que se caracteriza por una deformación tridimensional de la columna vertebral cuya etiología es aún desconocida. Actualmente no existe una explicación clara sobre la evolución de la enfermedad; El diagnóstico y seguimiento actual de la EIA se basa en el análisis de la curvatura de la columna mediante radiografía de rayos X, la cual está considerada como la técnica gold standard. Sin embargo, esta técnica no tiene valor pronóstico y las repetidas exposiciones han demostrado incrementar la incidencia de cáncer en la edad adulta. Es necesario, por tanto, el desarrollo de una nueva herramienta para el diagnóstico tem…
Long-Term Field Evaluation of Conventional vs. Micropropagated Plants of Chrysanthemum cinerariifolium
2022
Pyrethrum is a perennial herbaceous plant endemic to the eastern coast of the Adriatic Sea, and introduced in large areas of nearly all continents, where it is cultivated for the industrial extraction of pyrethrins. Pyrethrins are a group of six closely related monoterpene esters, widely used as natural insecticides. The world production of natural pyrethrins is lower than the market demand, and a wider introduction of this crop within the Mediterranean agrosystems could be an appealing opportunity for farmers and manufacturers. The availability of adequate amounts of selected plant material to bring into cultivation is, however, one of the major issues. Therefore, the in vitro propagation …
Influencia de la estimulación ovárica durante un tratamiento de fecundación in vitro en la incidencia de aneuploidías embrionarias
2014
La baja fecundidad humana podría deberse a la alta incidencia de anomalías cromosómicas embrionarias que justifica que hasta un 70% de las concepciones espontáneas no lleguen a término. La elevada tasa de aneuploidías observada en tratamientos de Fecundación in vitro (FIV) podría ser inherente a la especie humana o estar relacionada con los procedimientos derivados de la técnica, como las condiciones de cultivo in vitro o la estimulación ovárica (EO). Se ha especulado que esta última pudiera interferir con la selección natural de los ovocitos de buena calidad o que concentraciones suprafisiológicas de gonadotrofinas alteraran la maduración nuclear y la resunción de la meiosis conduciendo a …
Efectos de la estimulación ovárica en la calidad embrionaria, incidencia de aneuploidías y resultados clínicos
2012
La magnitud de la estimulación ovárica en el contexto de los tratamientos de fecundación in vitro podría tener efectos deletéreos sobre la calidad oocitaria y sobre la incidencia de aneuploidías. Material y método Estudio prospectivo de cohortes llevado a cabo en el Instituto Universitario Instituto Valenciano de Infertilidad de Valencia y el Instituto Valenciano de Infertilidad de Alicante. Un total de 32 donantes de oocitos altas respondedoras fueron sometidas a dos estimulaciones ováricas consecutivas con diferentes dosis de gonadotropinas: en la primera estimulación se administraron 225 UI de FSH-r diarias; en la segunda estimulación se emplearon 150 UI de FSH-r diarias. En ambos protoc…
Hairy Garlic (Allium subhirsutum) from Sicily (Italy): LC-DAD-MSn Analysis of Secondary Metabolites and In Vitro Biological Properties
2020
Allium subhirsutum, known as hairy garlic, is a bulbous plant widespread in the Mediterranean area and locally used as a food and spice. In the present study, the chemical profile of the ethanolic extracts from bulbs (BE) and aerial parts (APE) were analyzed by HPLC-ESI-MSn, and antioxidant properties were evaluated by DPPH, ABTS and TEAC assays. The traditional use in the diet, and the well documented biological activity of Allium species suggest a potential as a new nutraceutical. For this reason, the potential usefulness of this food can be considered in the treatment and prevention of degenerative Alzheimer disease. For this reason, acetylcholinesterase inhibitory property was investiga…
Is clinical experience important for obtaining the primary stability of dental implants with aggressive threads? An ex vivo study
2018
GECKILI, ONUR/0000-0002-7852-3915; dayan, suleyman cagatay/0000-0003-3081-9618; Bilhan, Hakan/0000-0003-1787-3003 WOS:000460131200015 PubMed ID: 30818319 Background: The aim of this study was to investigate the clinicians' experience on maintaining the primary stability of implants with aggressive threads belonging to a novel dental implant system. Material and Methods: Three hundred implants with aggressive threads were inserted in fresh bovine ribs mimicking Type IV bone by five clinicians which were classified according to their previous experience of total number of implant insertion. An independent examiner measured the primary stability of all implants after insertion by using resonan…
In vitro models of BBB: a tool for the analysis of cell to cell communication in the brain
2008
Many researchers have been trying to set in vitro models of the blood-brain barrier (BBB) aimed at analyzing, in simplified terms, the molecular mechanisms responsible for formation, maintenance and functioning of the BBB, as well as the capability of specific drugs and pro-drugs to cross BBB. We did it, starting with a simpler system of co-culture that allowed us to analyze the effects of neurons on differentiation of brain capillary endothelial cells (RBE4.B cells) in culture, and setting then a more complex model, that includes three cell types (endothelial cells, neurons and astrocytes). The reciprocal geometrical organization of brain cells in this model system is similar to the one ob…