Search results for "Vector"
showing 10 items of 2660 documents
Invasion biology and host specificity of the grapevine yellows disease vector Hyalesthes obsoletus in Europe
2008
Within the past 10 years, the yellows disease ‘bois noir’ (BN) has become one of the commercially most important diseases of grapevine [ Vitis vinifera L. (Vitaceae)] in Europe. Infection pressure is caused by phytoplasmas of the stolbur 16SrXII-A group that are transmitted by a planthopper vector, Hyalesthes obsoletus Signoret (Homoptera: Auchenorrhyncha). Infestation happens as an accidental side-effect of the feeding behaviour of the vector, as vector and pathogen proliferation is dependent on other plants. In Germany, the increase of BN is correlated with the use of a new host plant by the vector, increase in abundance of the vector on the new host plant, and dissemination of host plant…
The Sea Urchin sns5 Chromatin Insulator Improves the Likelihood of Lentiviral Vectors in Erythroid Milieu By Organizing an Independent Chromatin Doma…
2015
Abstract Retroviral vectors are currently the most suitable vehicles for therapeutic gene transfer in hematopoietic stem cells. However, these vectors are known to integrate rather randomly throughout the genome, suffering the so called chromosomal position effects (PE). Such a critical occurrence most probably depends upon the ability of heterochromatin to spread in the inserted vector sequences. Moreover, the use of transgenes imply genotoxicity effects, since the cis-regulatory sequences harbored by the vector can disturb the proper transcription of the resident genes neighboring the integration site, potentially leading to malignant transformation. Due to their enhancer blocker activity…
Nucleotide sequence of plasmid p4028, a cryptic plasmid from Leuconostoc oenos.
1996
Abstract TheLeuconostoc oenosplasmid p4028 was cloned in pBlueScript (SK+), and its complete nucleotide sequence was determined. The analysis of the nucleotide sequence revealed five open reading frames, all of them located on the same strand and grouped in two clusters separated by a short noncoding stretch. A similarity search against the other sequences deposited in the EMBL and GenBank databases showed that p4028 has no significant similarity with any of the sequences checked. Nevertheless, a putative ATP-binding motif was found in ORF2. A more detailed analysis of this ORF suggests that it could encode for a DNA-dependent ATPase.
Survival relative to new and ancestral host plants, phytoplasma infection, and genetic constitution in host races of a polyphagous insect disease vec…
2014
Dissemination of vectorborne diseases depends strongly on the vector's host range and the pathogen's reservoir range. Because vectors interact with pathogens, the direction and strength of a vector's host shift is vital for understanding epidemiology and is embedded in the framework of ecological specialization. This study investigates survival in host-race evolution of a polyphagous insect disease vector, Hyalesthes obsoletus, whether survival is related to the direction of the host shift (from field bindweed to stinging nettle), the interaction with plant-specific strains of obligate vectored pathogens/symbionts (stolbur phytoplasma), and whether survival is related to genetic differentia…
Investigating effects in GNSS station coordinate time series
2014
The vertical and horizontal displacements of the Earth can be measured to a high degree of precision using GNSS. Time series of Latvian GNSS station positions of both the EUPOS®-Riga and LatPos networks have been developed at the Institute of Geodesy and Geoinformation of the University of Latvia (LU GGI). In this study the main focus is made on the noise analysis of the obtained time series and site displacement identification. The results of time series have been analysed and distinctive behaviour of EUPOS®-Riga and LatPos station coordinate changes have been identified. The possible dependences of GNSS station coordinate distribution on EPN station problems, seismic activity of some area…
Effects of migratory status and habitat on the prevalence and intensity of infection by haemoparasites in passerines in eastern Spain
2013
Efectos del estatus migratorio y del tipo de habitat sobre la prevalencia y la intensidad de la infeccion por hemoparasitos en paseriformes en el este de Espana La peninsula iberica es un sitio idoneo para estudiar los efectos de la condicion migratoria en la prevalencia de hemoparasitos en comunidades de aves, dado que convergen poblaciones residentes locales con especies migratorias y abundantes poblaciones de vectores. En este trabajo examinamos la incidencia de hemoparasitos presentes en aves de tres localidades de la region mediterranea (este de Espana), con respecto del estatus migratorio. Examinamos 333 frotis sanguineos de 11 especies, y encontramos una prevalencia global del 9,6%. …
The baculovirus display technology--an evolving instrument for molecular screening and drug delivery.
2008
High throughput screening is a core technology in drug discovery. During the past decade, several strategies have been developed to screen (poly)peptide libraries for diverse applications including disease diagnosis and profiling, imaging, as well as therapy. The recently established baculovirus display vector system (BDVS) represents a eukaryotic screening platform that combines the positive attributes of both cell and virus-based display approaches, allowing presentation of complex polypeptides on cellular and viral surfaces. Compared to microbial display systems, the BDVS has the advantage of correct protein folding and post-translational modifications similar to those in mammals, facili…
Spectral analysis of the Neumann-Poincaré operator and characterization of the stress concentration in anti-plane elasticity
2012
When holes or hard elastic inclusions are closely located, stress which is the gradient of the solution to the anti-plane elasticity equation can be arbitrarily large as the distance between two inclusions tends to zero. It is important to precisely characterize the blow-up of the gradient of such an equation. In this paper we show that the blow-up of the gradient can be characterized by a singular function defined by the single layer potential of an eigenfunction corresponding to the eigenvalue 1/2 of a Neumann–Poincare type operator defined on the boundaries of the inclusions. By comparing the singular function with the one corresponding to two disks osculating to the inclusions, we quant…
Semisupervised nonlinear feature extraction for image classification
2012
Feature extraction is of paramount importance for an accurate classification of remote sensing images. Techniques based on data transformations are widely used in this context. However, linear feature extraction algorithms, such as the principal component analysis and partial least squares, can address this problem in a suboptimal way because the data relations are often nonlinear. Kernel methods may alleviate this problem only when the structure of the data manifold is properly captured. However, this is difficult to achieve when small-size training sets are available. In these cases, exploiting the information contained in unlabeled samples together with the available training data can si…
Effect of epicentral direction on seismic response of asymmetric buildings
1984
The paper deals with the influence of the epicentral direction on the displacement and stress response of multistorey asymmetric buildings to earthquake horizontal ground motion. A method is given for computing for each plane frame of the complex structure a particular direction of the bidirectional stationary random input for which the horizontal floor displacement of the given frame is maximized. It is shown that this direction can be considered conservative for the corresponding non-stationary process.