Search results for "Vibrio vulnificus"
showing 7 items of 97 documents
Eel immune response to Vibrio vulnificus infection. Host-pathogen relationship
2013
The European eel (Anguilla anguilla), has experienced a dangerous decline in recruitment, yield and stock over the last 30 years and this decline is likely to continue into the future. Several major threats are responsible for this situation, including overfishing of glass eels for consumption, new infections by introduced pathogens, dams and blocking of migration routes. The lack of knowledge concerning the biology of this species represents a handicap to the conservation and recovery of the population. Nevertheless,the eel farming industry is increasing, therefore it could be considered one of the major risks for wild eel stock perpetuation, which is threatened principally by the lack of …
Efficacy of oral reimmunisation after immersion vaccination against Vibrio vulnificus in farmed European eels
2004
Abstract Vulnivaccine, a licensed vaccine against Vibrio vulnificus , protects eels against vibriosis after vaccination by triple prolonged immersion at glass eel stage. Protection lasts for at least 6 months, but later, protection decreases, and eels can suffer stress-related vibriosis. The main objective of this work was to design an oral vaccine that can be used for reimmunisation at any developmental eel stage. With this aim, the efficacy of Vulnivaccine mixed with food as an oral booster was tested in an eel farm. The protection and the immune response (innate and acquired) in serum, mucus (from skin and intestine) and bile were evaluated in reimmunised and control animals (non-reimmun…
Susceptibility of Nile tilapia (Oreochromis niloticus) to vibriosis due to Vibrio vulnificus biotype 2 (serovar E)
2002
The present study documents the susceptibility of Nile tilapia to the experimental vibriosis caused by Vibrio vulnificus biotype 2 (serovar E) using a reference strain (Spanish Collection of Type Cultures, CECT 4604) selected for its high degree of virulence for eels. The biotype 1 of this species is one of the usual organisms involved in epizootics occurred in tilapia. After intraperitoneal injection, the selected strain developed a haemorrhagic septicaemia similar to eel vibriosis with a LD50 four log units lower than that exhibited by the type strain of the species, which belongs to the biotype 1. The results obtained in waterborne and intubation challenges indicated that water and feed …
Influence of aquatic microbiota on the survival in water of the human and eel pathogen Vibrio vulnificus serovar E
2004
Summary The eel and human pathogen Vibrio vulnificus serovar E (biotype 2) is seldom isolated from natural waters, although it can survive in sterilized artificial seawater microcosms for years. The main objective of the present study was to investigate whether aquatic microbiota can limit its survival and recovery from water samples. A set of preliminary experiments of survival in microcosms containing natural seawater and water from eel farms showed that the persistence of this pathogen was mainly controlled by grazing, and secondarily by bacterial competition. The bacte- rial competition was further analysed in artificial seawater microcosms co-inoculated with selected virulent serovar E…
Plasmid diversity in Vibrio vulnificus biotypes.
2009
Vibrio vulnificus is a heterogeneous bacterial species that can be virulent for humans and fish. Virulence in fish seems to rely on a recently described plasmid that can be transmitted between strains, aided by a conjugative plasmid. The main objective of this work was to analyse the plasmid content of a wide collection of strains from the three biotypes of the species, as well as to identify putative conjugative and virulence plasmids by means of Southern hybridization with specific probes and sequence analysis of selected gene markers. We found 28 different plasmid profiles in a total of 112 strains, which were relatively biotype- or serovar-specific. Biotype 1 lacked high-molecular-mass…
Vibrio vulnificus: from water to host
2017
Vibrio vulnificus is an aquatic pathogen autochthonous from temperate, tropical and subtropical ecosystems where it lives either as a sessile cell, forming biofilms or as a free-swimming cell. From these locations, the pathogen can occasionally infect humans and fish causing a disease named vibriosis. The most severe form of human and fish vibriosis is associated with the pathogen’s ability to spread from the infection site to the bloodstream and multiply, process known as invasion. Before invasion, the pathogen has to colonize the mucosal host surface, process that involves not only bacterial attachment/adhesion but also resistance to mucosal immunity, commensal microbiota (competitors) an…
Transcriptomic analysis of host-pathogen relationship in Vibrio vulnificus
2020
Vibrio vulnificus es un patógeno bacteriano que habita en ecosistemas costeros de salinidades intermedias localizados en zonas del planeta templadas y cálidas. Esta especie es una de las más sensibles al calentamiento global habiendo experimentado una expansión en su distribución geográfica hacia zonas costeras de aguas frías como las que rodean países tan próximos al círculo polar como como Noruega y Groenlandia, de las que se aísla en las épocas cálidas del año. En su medio natural la bacteria puede encontrarse en un estado metabólico inactivo, conocido como estado VBNC (viable but not culturable) que es inducido en circunstancias ambientales adversas (temperaturas por debajo de 10ºC, sal…