Search results for "Vitellogenin"

Biomarker responses by crucian carp () living in a pond of secondary treated pulp mill effluent

1999

Biochemical and histological biomarkers by the adult crucian carp ( Carassius carassius (L.)) living in a biologically treated pulp mill effluent were studied. Enocell pulp mill in Uimaharju, Finland, discharges its effluents through a waste water pond to the River Pielisjoki. This pond harbours an introduced crucian carp population that apparently reproduces on site. The objective of the present study was to measure possible hormonal effects of treated pulp mill effluent on crucian carps living in a wastewater pond. Adult fish were collected for analysis by traps. A reference population was sampled from a small natural pond. Blood and bile samples were taken for analyses of hemoglobin, hem…

Advancing the ecotoxicological relevancy of zebrafish : application of early-juvenile 20dpfZF to assess xenoestrogenicity of environmental chemicals …

2014

The non-appearance of female-specific proteins in male Spilostethus pandurus)

1990

1. 1. Female-specific peptides can be detected by SDS-PAGE in the haemolymph when the female insects are 48 hr old. 2. 2. The age of the male insect at the moment of treatment with juvenile hormone I (JH I), JH III or ecdysone has no influence on the induction of the female-specific bands in male S. pandurus. 3. 3. Male insects exposed to the hormones for periods of time longer than 24 hr were also unable to show female-specific bands in their haemolymph. Consecutive treatments with JH were unable to induce these peptides in the males.

Glutathionyl-biliverdin IXα, a new heme catabolite in a marine annelid: Sex and cell specific accumulation.

2011

Abstract We have isolated and characterized the green pigment accumulating in coelomic cells (eleocytes) of the common clam worm Nereis virens by means of RP-HPLC and ESI-tandem mass spectrometry. This pigment is a novel biliverdin-glutathione conjugate in which the glutathione is linked to the biliverdin-backbone via a thioether bond. The yolk precursor vitellogenin, a female-specific high-density lipoprotein ( ρ  = 1179 kg/m 3 ) with a native molecular mass of ∼500 kDa and a subunit mass of ∼150 kDa, is capable of transporting this pigment as well as heme. The sex-independent large discoidal lipoprotein present in the coelomic fluid, which is sequestered by the eleocytes, could also be sh…

Analysis of involvement of the 3?-untranslated regions in regulating mRNA stability for vitellogenin, cyanoprotein ?, and cyanoprotein ? from the bea…

2002

The degradation of the 3'-untranslated regions (UTRs) of vitellogenin, cyanoprotein alpha, and cyanoprotein beta from the bean bug, Riptortus clavatus, was analyzed in vitro. The degradation pattern was similar for all three RNAs, with a high degradation rate in non-diapausing adult insects and no degradation in the fifth instar nymphs and in diapausing adults, and was not correlated with the expression levels of these three proteins. Proteins binding to the 3'-UTRs were detected in polysomal and cytosolic extracts. These factors, however, were present in all developmental stages. The abundance of the polysomal factor showed little variation, but the cytosolic factor was enriched in adult i…

Use of Early Juvenile Zebrafish Danio Rerio for In-Vivo Assessment of Endocrine Modulation by Xenoestrogens

2013

Reliable and cost-effective early-life stage (ELS) bioassays incorporating practical experimentation without compromising scientific relevance are crucial in chemical risk assessment. This study investigated the use of 20 days- post-fertilization life stage ( 20dpfZF ) of zebrafish Danio rerio to screen environmental chemicals known to be estrogenic in adult fish. Firstly, studies with key genes in steroidogenesis were conducted; the brain isoform of aromatase gene ( cyp19a1b ) being the most prominently expressed biomarker. Regulation of mRNA levels of molecular biomarkers, vitellogenin 1 gene ( vtg1) and cyp19a1b were selected to assess the endocrine modulation by xenoestrogens, 17α-ethin…

Annelid Coelomic Fluid Proteins

2020

The coelomic cavity is part of the main body plan of annelids. This fluid filled space takes up a considerable volume of the body and serves as an important site of exchange of both metabolites and proteins. In addition to low molecular substances such as amino acids and glucose and lactate, the coelomic fluid contains different proteins that can arise through release from adjacent tissues (intestine) or from secretion by coelomic cells. In this chapter, we will review the current knowledge about the proteins in the annelid coelomic fluid. Given the number of more than 20,000 extant annelid species, existing studies are confined to a relatively few species. Most studies on the oligochaetes …

Honey Bee Suppresses the Parasitic Mite Vitellogenin by Antimicrobial Peptide

2020

AbstractThe negative effects of honey bee parasitic mites and deformed wing virus (DWV) on honey bee and colony health have been well characterized. However, the relationship between DWV and mites, particularly viral replication inside the mites, remains unclear. Furthermore, the physiological outcomes of honey bee immune responses stimulated by DWV and the mite to the host (honey bee) and perhaps the pathogen/parasite (DWV/mite) are not yet understood. To answer these questions, we studied the tripartite interactions between the honey bee,Tropilaelaps mercedesae, and DWV as the model.T. mercedesaefunctioned as a vector for DWV without supporting active viral replication. Thus, DWV negligib…

Sialotranscriptomics of the argasid tick ornithodoros moubata along the trophogonic cycle

2021

32 páginas, 8 tablas, 6 figuras

Use of Early Life-Stages of Zebrafish to Assess Toxicity of Sediments Contaminated by Organotin Compounds

2016

ABSTRACTThis study examined the response of early life-stages (ELS) of zebrafish to organotin-contaminated sediment from Lake Huruslahti (HL) in Central Finland. A dilution series (0, 10, 33, and 100%) of the native (HL) and the sediment spiked with tributyltin (TBT) determined a dose-response of zebrafish ELS to organotin-contaminated sediment. Sediment elutriates were assessed by bacterial bioluminescence assay and microscopical pathologies of 1–3 days post-fertilization zebrafish (1–3dpfZF). Brain aromatase (cyp19a1b) and tissue vitellogenin (vtg1) were assayed from early-juvenile zebrafish (20dpfZF) exposed to intact sediment. In vivo modulation of cyp19a1b and vtg1 transcripts in 20dpf…