Search results for "Vitis"
showing 10 items of 457 documents
An ex-vivo model for transsynovial drug permeation of intraarticular injectables in naive and arthritic synovium
2021
Abstract Estimation of joint residence time of a drug is a key requirement for rational development of intraarticular therapeutics. There is a great need for a predictive model to reduce the high number of animal experiments in early stage development. Here, a Franz-cell based porcine ex-vivo permeation model is proposed, and transsynovial permeation of fluorescently-labeled dextrans in the range of potential drug candidates (10–150 kDa), as well as a small molecule (fluorescein sodium) and charged dextran derivates, have been determined. In addition, a lipopolysaccharide (LPS) -induced synovitis model was assessed for inflammatory biomarker levels and its effect on permeation of the solute…
Analysis of protein composition of red wine in comparison with rosé and white wines by electrophoresis and high-pressure liquid chromatography-mass s…
2009
Wine proteins not only influence wine stability but are also being discussed as potential allergens. Proteins from red, rose, and white wines were enriched by dialysis and lyophilization followed by separation by SDS-PAGE. Significant differences were detected in the protein compositions of the analyzed wine varieties, and the major protein bands were identified by mass spectrometry after in-gel digestion with trypsin. In German Portugieser red wine, a total of 121 tryptic peptides were identified, which were attributed to 12 grape proteins and 6 proteins derived from yeast. Among the identified constituents are several proteins considered to influence wine stability and previously describe…
Lesiones de la mucosa bucal y comportamiento de la enfermedad periodontal en embarazadas
2004
-Objetivos. Identificar la prevalencia y severidad de enfermedad periodontal, necesidades de tratamiento periodontal y prevalencia de lesiones de la mucosa bucal. -Diseño del estudio. Estudio prospectivo, observacional y tangencial en el que se examinaron 7,952 mujeres (1991-1999) en la Escuela de Odontología de la Universidad De La Salle Bajío, en León, Gto. México, registrándose: edad, antecedentes personales patológicos, índice periodontal simplificado y lesiones de la mucosa bucal. Se integraron dos grupos: de mujeres embarazadas y de no gestantes, con edades similares. Los resultados fueron manejados en porcentajes, utilizando la prueba de Chi cuadrada para identificar su significancia…
Runoff initiation, soil detachment and connectivity are enhanced as a consequence of vineyards plantations.
2017
[EN] Rainfall-induced soil erosion is a major threat, especially in agricultural soils. In the Mediterranean belt, vineyards are affected by high soil loss rates, leading to land degradation. Plantation of new vines is carried out after deep ploughing, use of heavy machinery, wheel traffic, and trampling. Those works result in soil physical properties changes and contribute to enhanced runoff rates and increased soil erosion rates. The objective of this paper is to assess the impact of the plantation of vineyards on soil hydrological and erosional response under low frequency - high magnitude rainfall events, the ones that under the Mediterranean climatic conditions trigger extreme soil ero…
The distribution of Rare Earth Elements discriminates the growth substrate of Vitis vinifera L.
2021
Sustainable agricultural, food-related strategies and geographic traceability require understanding of the plant physiological response to stress potentially generated by contaminated soils. Here, we have investigated the effect of contaminated substrate on growth of Vitis vinifera L. plants analysing the distribution of full Rare Earth Elements (REE) spectra in different parts of the plant. Experiments were carried out using pristine plants growing in a handmade substrate (blank experiment) and in REE artificially-enriched soil (spiked experiment). Our results show that both plant mass and REE amount in leaves are not influenced by the substrate enrichment while roots are by one-order of m…
An ethoxylated surfactant enhances the penetration of the sulfated laminarin through leaf cuticle and stomata, leading to increased induced resistanc…
2016
International audience; Some β-1,3-glucans and particularly sulfated laminarin (PS3) are known as resistance inducers (RIs) in grapevine against the downy mildew. However, their efficacy in vineyard is still often too low, which might be caused by a limited penetration through the leaf cuticle following spray application. We used (14) C-sucrose uptake experiments with grapevine leaves in order to select a surfactant as saccharide penetration enhancer. Our results showed that though sucrose foliar uptake was low, it was strongly enhanced by Dehscofix CO125 (DE), a highly ethoxylated surfactant. Fluorescent saccharides were then produced and laser scanning microscopy was used to analyze their…
Supercritical fluid extraction and HPLC determination of relevant polyphenolic compounds in grape skin.
2005
The polyphenols determined are: (+)-catechin, (-)-epicatechin, rutin, quercetin and trans-resveratrol. Suitable conditions of supercritical fluid extraction were established using ethanol as a modifier of the polarity solvent (supercritical carbon dioxide). Final extraction conditions were: 20% v/v ethanol, 60degreesC, 250 bars and flow rate 2 mL/min. Static step time and dynamic step time were established using a selected grape skin sample. The extract was collected in water; the more polar polyphenols ((+)-catechin and (-)-epicatechin) remain in solution but rutin, quercetin and trans-resveratrol precipitate in this medium, thereby the solution of the extracted polyphenols was filtered. (…
Chemical and spectroscopic characteristics of the wood of Vitis vinifera cv. Sangiovese affected by esca disease.
2009
Chemical and spectroscopic analyses ((13)C cross-polarization-magic angle spinning NMR and attenuated total reflection Fourier transform infrared spectroscopies) were carried out on the wood of Vitis vinifera cv. Sangiovese with brown-red discoloration and black streaks caused by esca disease. The analyses of the brown-red wood revealed the destruction of hemicelluloses and noncrystalline cellulose as well as modifications in the pectic and ligninic wood fractions. The pectic fraction consisted of carbohydrates associated with polyphenols. The lignin fraction exhibited only a few changes in the aromatic systems and a partial demethylation, and it appeared to be associated with condensed phe…
Behaviour of REE in the soil/Vitis Vinifera L. system. Geochemical Approach for Food Traceability
2021
The geographic traceability of food products through the use of chemical markers is an important challenge to ensure quality and authenticity of food. In recent years, the behaviour of Rare Earth Elements (REE) has been identified as possible tool for food geographical identification based on their known capability of tracing pedo-genetic and petro-genetic processes. In this thesis, the behaviour of REE in the Soil/Vitis vinifera L. system has been explored using a geochemical approach. The goal is to understand if the normalized pattern of REE (REE*) can be a useful tool to trace the geographical origin of food. REE may be accumulated in plants keeping their distribution in passing from so…
Classification of varieties for their timing of flowering and veraison using a modelling approach: A case study for the grapevine species Vitis vinif…
2013
16 pages; International audience; tUnderstanding differences in phenology for varieties of a given species is important for making informeddecisions on variety choice in the context of climate change and breeding new cultivars. Phenologicalmodels that incorporate temperature as a key environmental factor can be used to describe the differencesin phenological timing between cultivars. This paper outlines a methodology, based on a phenologicalmodel, for classifying the timing of flowering and veraison for a substantial number of varieties of thegrapevine (Vitis vinifera L.). 95 varieties were characterized for flowering and 104 varieties for veraison.Various statistical measures were used to …