Search results for "WES"
showing 10 items of 1585 documents
Pharmacogenomics: questions and concerns
2005
The progressively aging population in the western world, rising socioeconomic expenditure and increasing costs for the treatment of adverse drug reactions, lead to increasing pressure on public spending. The public acceptance of pharmacogenomics is high, therefore, because it promises individualized safe and effective treatment at lower cost. Pharmacogenomics studies the genetic polymorphisms that underlie the variability in drug response between individuals. Despite the great benefits being awaited from this new field, a number of ethical, social and legal concerns arise, which demand rapid strict international regulations in order to prevent discrimination or harm of any kind from society…
Evidence for a direct interaction of Rev protein with nuclear envelop mRNA-translocation system.
1991
The interaction of the Rev protein from human immunodeficiency virus type 1 (HIV-1) with the nucleocytoplasmic mRNA-transport system was investigated. In gel-shift assay, the recombinant Rev protein used in this study selectively bound to the Rev-responsive element (RRE) region of HIV-1 env-specific RNA. Nitrocellulose-filter-binding studies and Northern/Western-blotting experiments revealed an association constant of approximately 1 x 10(10) M-1. The Rev protein also strongly bound to isolated nuclear envelopes from H9 cells, containing the poly(A)-binding site (= mRNA carrier) and the nucleoside triphosphatase (= NTPase), which are thought to be involved in nuclear export of poly(A)-rich …
Peasants and the Political Culture in Norway (c. 1400–1700)
2016
This chapter looks at the major confrontations between the state and peasants in Norway during the period 1400–1700. These have variously been described as rebellions, riots and insurrections. Indeed, one could argue that none of these were full-scale revolts as such, if by that we mean a massive threat to the regime and social order. The peasants and their leaders would never declare that they were seeking to abolish the regime or attack the king nor that they intended to implement a new social or political order. In most cases, they would address oral or written protests or sabotage the collection of new taxes or other burdens that the state had imposed. Their protests would primarily be …
Demonstration of an endocrine signaling circuit for insulin in the sponge Geodia cydonium.
1989
Abstract The existence of an insulin-mediated cell-to-cell signaling in the sponge Geodia cydonium is demonstrated in this study by molecular biological and immunological techniques. The sequence of a sponge cDNA clone encoding preproinsulin was analyzed for the first time and determined to comprise a high homology to human preproinsulin (60-80% homology). The predicted polypeptide of preproinsulin from sponge contains two disulfide bridges which link the A- to the B-chain. The intra-A chain disulfide bridge is absent. Applying immunological and electron microscopical techniques it is shown that insulin is produced in specialized cells (spherulous cells). Experimental evidence is presented …
3D SINGLETONS AND THEIR BOUNDARY 2D CONFORMAL FIELD THEORY
1999
This paper is a continuation of recent work of Flato and Frønsdal on singletons in 1+2 anti De Sitter universe and their link with 2D conformal field theories on the boundary. More specifically we show that in this framework we can construct a 3D-singleton model in the bulk, the limit of which on the boundary of De Sitter space is a Gupta–Bleuler triplet for two commuting copies of the Witt algebra. We also generalize this result to the case of WZNW models.
De Novo prion aggregates trigger autophagy in skeletal muscle
2014
ABSTRACT In certain sporadic, familial, and infectious prion diseases, the prion protein misfolds and aggregates in skeletal muscle in addition to the brain and spinal cord. In myocytes, prion aggregates accumulate intracellularly, yet little is known about clearance pathways. Here we investigated the clearance of prion aggregates in muscle of transgenic mice that develop prion disease de novo . In addition to neurodegeneration, aged mice developed a degenerative myopathy, with scattered myocytes containing ubiquitinated, intracellular prion inclusions that were adjacent to myocytes lacking inclusions. Myocytes also showed elevated levels of the endoplasmic reticulum chaperone Grp78/BiP, su…
Interaction of 68–kDa TAR RNA-binding protein and other cellular proteins with rpion protein-RNA stem-loop
1995
The RNA stem-loop structure of the trans-activating region TAR sequence of human immunodeficiency virus-1 mRNA is the binding site for a number of host cell proteins. A virtually identical set of proteins from HeLa nuclear extracts was found to bind to the predicted RNA hairpin element of prion protein (PrP) mRNA, as demonstrated in UV cross-linking/RNase protection and Northwestern assays. We show that the cellular TAR loop-binding protein, p68, is among those proteins which associate with PrP RNA. Competition experiments with various TAR RNA mutants revealed that binding of partially purified p68 to PrP RNA stem-loop occurs sequence-specifically. The 100-kDa 2-5A synthetase which is invol…
Facebook’s Emotional Contagion Experiment as a Challenge to Research Ethics
2016
This article analyzes the ethical discussion focusing on the Facebook emotional contagion experiment published by the <em>Proceedings of the National Academy of Sciences</em> in 2014. The massive-scale experiment manipulated the News Feeds of a large amount of Facebook users and was successful in proving that emotional contagion happens also in online environments. However, the experiment caused ethical concerns within and outside academia mainly for two intertwined reasons, the first revolving around the idea of research as manipulation, and the second focusing on the problematic definition of informed consent. The article concurs with recent research that the era of social med…
Localizing the politics of privacy in communication and media research
2020
While previous communication and media research has largely focused on either studying privacy as personal boundary management or made efforts to investigate the structural (legal or economic) condition of privacy, we observe an emergent body of research on the political underpinnings of privacy linking both aspects. A pronounced understanding of the politics of privacy is however lacking. In this contribution, we set out to push this forward by mapping four communication and media perspectives on the political implications of privacy. In order to do so, we recur on Barry’s (2002) distinction of the political and the politics and outline linkages between individual and structural dimensions…
The Politics of Privacy - a Useful Tautology
2020
While communication and media studies tend to define privacy with reference to data security, current processes of datafication and commodification substantially transform ways of how people act in increasingly dense communicative networks. This begs for advancing research on the flow of individual and organizational information considering its relational, contextual and, in consequence, political dimensions. Privacy, understood as the control over the flow of individual or group information in relation to communicative actions of others, frames the articles assembled in this thematic issue. These contributions focus on theoretical challenges of contemporary communication and media privacy …