Search results for "WINE"
showing 10 items of 1468 documents
Fungal biodiversity in a new winery and population dynamics in the winery (Saccharomyces cerevisiae) and in aging cellar (Brettanomyces bruxellensis)
2020
The interspecific fungal biodiversity (Illumina Mi-Seq) and the dynamics of Saccharomyces cerevisiae and Brettanomyces bruxellensis species were studied in a new winery and/or in 3 aging cellars, more specifically on the floor, the walls, the equipment and the outside of the barrels. In the new winery, an initial fungal consortium (yeasts and molds) is already present on all the winery environments before the arrival of the first harvest. This consortium consists of fungal genera such as Aureobasidium, Alternaria, Didymella and Filobasidium. These genera, that persist during two vintages, are not specific to the winery environment and seem to be adapted to natural or anthropic environments …
Evolution of microbiological and chemical parameters during red wine making with extended post-fermentation maceration.
2014
Abstract The aim of the present work was to investigate the microbiological, chemical, and sensory characteristics of red wine subjected to post-fermentation maceration that was extended to 90 days. For this purpose, the ‘Aglianico di Taurasi’ grape was used as a case study. The total yeast concentration increased until day 40 of maceration and decreased thereafter, whereas the concentration of lactic acid bacteria slightly increased. Dekkera/Brettanomyces spp. and acetic acid bacteria were not detected. The yeast community was composed of Saccharomyces cerevisiae, Zygosaccharomyces bisporus, Metschnikowia pulcherrima, Hanseniaspora guilliermondii, Hanseniaspora uvarum, Pichia guilliermondi…
Design and performance testing of a real-time PCR assay for sensitive and reliable direct quantification of Brettanomyces in wine
2009
International audience; Because the yeast Brettanomyces produces volatile phenols and acetic acid, it is responsible for wine spoilage. The uncontrolled accumulation of these molecules in wine leads to sensorial defects that compromise wine quality, The need for a rapid, specific, sensitive and reliable method to detect this spoilage yeast has increased over the last decade. All these requirements are met by real-time PCR. We here propose improvements of existing methods to enhance the robustness of the assay. Six different protocols to isolate DNA from a wine and three PCR mix compositions were tested, and the best method was selected. Insoluble PVPP addition during DNA extraction by a cla…
Sorption of 4-ethylphenol and 4-ethylguaiacol by suberin from cork
2015
International audience; Cork shows an active role in the sorption of volatile phenols from wine. The sorption properties of 4-ethylphenol and 4-ethylguaiacol phenols in hydro-alcoholic medium placed in contact with suberin extracted from cork were especially investigated. To that purpose, suberin was immersed in model wine solutions containing several concentrations of each phenol and the amount of the compound remaining in the liquid phase was determined by SPME-GC-MS. Sorption isotherms of 4-ethylguaiacol and 4-ethylphenol by suberin followed the Henry's model. The solid/liquid partition coefficients (KSL) between the suberin and the model wine were also determined for several other volat…
Le vin de Dijon du XIVe au XVIIIe siècle : trajectoire historique d’un cru (presque) disparu
2012
International audience
Investigation of sterols as potential biomarkers for the detection of pig (S. s. domesticus) decomposition fluid in soils
2012
This study was carried out to evaluate the potential of using cholesterol and coprostanol, as indicators for the detection of decomposition fluid of buried pigs (S. s. domesticus) in soils. In May 2007, four pig carcasses (~35. kg) were buried in shallow graves (~40. cm depth) at the University of Ontario Institute of Technology in Canada. Two pigs were exhumed after three months (Pig 1, Pig 2) and six months (Pig 3, Pig 4) post burial. Soil samples were collected beneath the pig carcasses (~40. cm depth) and from grave walls (~15-20. cm depth) as well as from a parallel control site. Coprostanol and cholesterol were extracted from soils, purified with solid phase extraction (SPE) and analy…
Histomorphological study of the bone regeneration capacity of platelet-rich plasma, bone marrow and tricalcium phosphate Experimental study on pigs
2009
Introduction: Bone defects are rather common after oral surgery and may prove difficult to repair. Objective: We provide a histomorphological analysis of the bone regenerative capacity of platelet-rich plasma at different concentrations and the extraction of platelet-rich bone marrow, compared with -tricalcium phosphate. Methodology: We performed an experimental study on 8 pigs, in which we performed trepanations of the mandible in order to place the materials to be studied. Using an electron microscope, we observed the samples obtained and took a series of photographs in order to analyze the samples through a gray-scale histogram system. Results: Ossification phenomena were present in 96% …
Calcium Phosphate-Coated Titanium Implants in the Mandible: Limitations of the in vivo Minipig Model
2020
<b><i>Introduction:</i></b> We aimed to compare implant osseointegration with calcium phosphate (CaP) surfaces and rough subtractive-treated sandblasted/acid etched surfaces (SA) in an in vivo minipig mandible model. <b><i>Materials and Methods:</i></b> A total of 36 cylindrical press-fit implants with two different surfaces (CaP, <i>n</i> = 18; SA, <i>n</i> = 18) were inserted bilaterally into the mandible of 9 adult female minipigs. After 2, 4, and 8 weeks, we analyzed the cortical bone-to-implant contact (cBIC; %) and area coverage of bone-to-implant contact within representative bone chambers (aBIC; %). <b><…
Effects of resveratrol analogs on cell cycle progression, cell cycle associated proteins and 5fluoro-uracil sensitivity in human derived colon cancer…
2009
International audience; Epidemiological studies suggested that trans-resveratrol, a wine grape component, could prevent malignant tumor development. This compound also demonstrated cytostatic and cytotoxic effects on tumor cells in vitro. To obtain trans-resveratrol derivatives with a better cellular uptake and enhanced antiproliferative effects, we synthesized a triacetate derivative as well as an oligomer, epsilon-viniferin and its acetylated form, epsilon-viniferin penta-acetate. We also obtained vineatrol, a wine grape shoot extract that associates several polyphenols that may act synergistically, including trans-resveratrol and epsilon-viniferin. We show here that resveratrol triacetat…
Repetitive nucleotide sequencing of a dispensable DNA segment in a clonal population of African swine fever virus
1991
Abstract Repetitive nucleotide sequencing of a dispensable genomic segment of a clonal population of African swine fever (ASF) virus has been carried out to estimate the mutant frequency to neutral alleles. Since no mutations have been detected in a total of 54026 nucleotides screened, the maximum mutant frequency is 5.5 × 10 −5 substitutions/nucleotide (95% confidence level). The result renders very unlikely the occurrence of hypermutational events during ASF virus DNA replication, at least within the selected DNA fragment.