Search results for "Western"

showing 10 items of 1138 documents

Modeling Human Endometrial Decidualization from the Interaction between Proteome and Secretome

2011

Context: Decidualization of the human endometrium, which involves morphological and biochemical modifications of the endometrial stromal cells (ESCs), is a prerequisite for adequate trophoblast invasion and placenta formation. Objective: This study aims to investigate the proteome and secretome of in vitro decidualized ESCs. These data were combined with published genomic information and integrated to model the human decidualization interactome. Design: Prospective experimental case–control study. Setting: A private research foundation. Patients: Sixteen healthy volunteer ovum donors. Intervention: Endometrial samples were obtained, and ESCs were isolated and decidualized in vitro. Main Ou…

AdultProteomicsmedicine.medical_specialtyAdolescentProteomeEndocrinology Diabetes and MetabolismBlotting WesternClinical BiochemistryEnzyme-Linked Immunosorbent AssayBiologyProteomicsModels BiologicalBiochemistryInteractomeEndometriumYoung AdultEndocrinologyCytokines metabolismInternal medicineDeciduamedicineHumansCell shapeCell ShapeCells CulturedBiochemistry (medical)DeciduaComputational BiologyDecidualizationmedicine.anatomical_structureEndocrinologyPost translationalCase-Control StudiesCulture Media ConditionedSpectrometry Mass Matrix-Assisted Laser Desorption-IonizationProteomeCytokinesElectrophoresis Polyacrylamide GelFemaleStromal CellsProtein Processing Post-TranslationalBiomarkersThe Journal of Clinical Endocrinology & Metabolism
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Pleural Mesothelial Cells Express Both BLT2 and PPARα and Mount an Integrated Response to Pleural Leukotriene B4

2008

Abstract Leukotriene B4 (LTB4) plays a crucial role in the recruitment of neutrophils into the pleural space. We identified for the first time the mechanisms by which LTB4 interacts with mesothelial cells and recruits neutrophils in the pleural compartment. Primary pleural mesothelial cells express both the proinflammatory receptor for LTB4 BLT2, and the anti-inflammatory receptor for LTB4, PPARα. Parapneumonic pleural effusions highly increase BLT2 expression and, via BLT2 activation, increase the adhesion between mesothelial cells and neutrophils and the expression of ICAM-1 on mesothelial cells. The block of PPARα further increases both cell adhesion and ICAM-1 expression. BLT2 activatio…

Adultmedicine.drug_classLeukotriene B4Blotting WesternImmunologyReceptors Leukotriene B4Protein tyrosine phosphataseBiologyLeukotriene B4EpitheliumTyrosine-kinase inhibitorProinflammatory cytokinechemistry.chemical_compoundCell AdhesionmedicineHumansImmunology and AllergyPPAR alphaCell adhesionCells CulturedAgedInflammationLeukotriene B4 receptorPneumoniaMiddle Agedrespiratory systemFlow CytometryIntercellular Adhesion Molecule-1respiratory tract diseasesPleural EffusionChemotaxis LeukocyteNeutrophil InfiltrationchemistryImmunologyCancer researchPleuraPhosphorylationMesothelial CellThe Journal of Immunology
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Uran aus deutschen Nuklearprojekten der 1940er Jahre – eine nuklearforensische Untersuchung

2015

Wir berichten hier uber eine nuklearforensische Analyse verschiedener Uranmaterialien aus deutschen Nuklearprojekten der 1940er Jahre, bei denen Uranmetall in verschiedenen Geometrien verwendet wurde. Mithilfe des 230Th/234U-Isotopenverhaltnisses konnte fur das Metall ein Produktionszeitraum von 1940 bis 1943 bestimmt werden. Die geographische Herkunft des Urans wurde anhand des Spurengehalts an Seltenerdelementen und der Strontium-Isotopenverhaltnisse bestimmt. Die Ergebnisse zeigen, dass das bei den deutschen Nuklearprojekten verwendete Uran aus Minen in der Tschechischen Republik stammt. 236U und 239Pu wurden in Spuren nachgewiesen. Diese entsprechen in etwa der Haufigkeit dieser Isotope…

Age estimationWestern europeFederal republic of germanyGeneral MedicinePlutonium isotopesHumanitiesAngewandte Chemie
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Aging Negatively Affects Estrogens-Mediated Effects on Nitric Oxide Bioavailability by Shifting ERα/ERβ Balance in Female Mice

2011

AIMS: Aging is among the major causes for the lack of cardiovascular protection by estrogen (E2) during postmenopause. Our study aims to determine the mechanisms whereby aging changes E2 effects on nitric oxide (NO) production in a mouse model of accelerated senescence (SAM). METHODS AND RESULTS: Although we found no differences on NO production in females SAM prone (SAMP, aged) compared to SAM resistant (SAMR, young), by either DAF-2 fluorescence or plasmatic nitrite/nitrate (NO2/NO3), in both cases, E2 treatment increased NO production in SAMR but had no effect in SAMP. Those results are in agreement with changes of eNOS protein and gene expression. E2 up-regulated eNOS expression in SAMR…

AgingAnatomy and Physiologylcsh:MedicineEstrogen receptorFluorescent Antibody TechniqueCardiovascularCardiovascular SystemBiochemistrychemistry.chemical_compoundMiceEndocrinologyEnosMolecular Cell BiologyMembrane Receptor Signalinglcsh:ScienceReceptorMultidisciplinarybiologySuperoxideNeurochemistryHormone Receptor SignalingReceptors EstrogenDNA methylationCirculatory PhysiologyMedicineFemaleNeurochemicalsResearch ArticleSignal TransductionSenescencemedicine.medical_specialtymedicine.drug_classBlotting WesternEndocrine SystemNitric OxideReal-Time Polymerase Chain ReactionCardiovascular PharmacologyNitric oxideInternal medicinemedicineCardiovascular Diseases in WomenAnimalsBiologyEndocrine Physiologylcsh:RNADPH OxidasesEstrogensDNA Methylationbiology.organism_classificationHormonesEndocrinologychemistryEstrogenWomen's Healthlcsh:QNeurosciencePLoS ONE
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Impairment of intracellular antiviral defense with age: age-dependent changes in expression of interferon-induced and double-stranded RNA-activated 2…

1995

The 2',5'-oligoadenylate (2-5A) system is involved in the defense of mammalian cells against virus infection. In a previous study [25], we demonstrated that the activities of the enzymes which synthesize and degrade 2-5A [2-5A synthetase (2-5OAS) and 2',3'-exoribonuclease] and of the enzyme that is activated by 2-5A (ribonuclease L) change during aging and development in different tissues of rat. The age-dependent decrease in 2-5OAS activity and increase in 2-5A nuclease activity results in a decrease in the cellular 2-5A content, suggesting that the efficiency of the antiviral 2-5A system is impaired in aged rats. Here we determined the age-dependent changes in the level of mRNA coding for…

AgingBlotting WesternMolecular Sequence DataBiologyIsozymeInterferonmedicine2'5'-Oligoadenylate SynthetaseAnimalsAmino Acid SequenceRNA MessengerRNA Double-Strandedchemistry.chemical_classificationMessenger RNAActivator (genetics)Age FactorsRNABrainBiological activityMolecular biologyRatsEnzymechemistryBiochemistryLiverVirus Diseasesbiology.proteinFemaleInterferonsProtein KinasesRibonuclease LDevelopmental Biologymedicine.drugMechanisms of ageing and development
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Endothelin Antagonism: Effects of FP Receptor Agonists Prostaglandin F2αand Fluprostenol on Trabecular Meshwork Contractility

2006

Purpose This study analyzes additional mechanisms behind the ocular hypotensive effect of prostaglandin F (PGF) receptor (FP receptor) agonists PGF2alpha and fluprostenol (fluprostenol-isopropyl ester [travoprost]), which reduce intraocular pressure (IOP) in patients with glaucoma probably by enhancing uveoscleral flow. The trabecular meshwork (TM) is actively involved in IOP regulation through contractile mechanisms. Contractility of TM is induced by endothelin (ET)-1, a possible pathogenic factor in glaucoma. The involvement of FP receptor agonists in the ET-1 effects on TM function was studied. Methods The effects of FP receptor agonists on contractility of bovine TM (BTM) were investiga…

Agonistmedicine.medical_specialtyCarbacholmedicine.drug_classBlotting WesternReceptors ProstaglandinDinoprostContractilityTravoprostTrabecular MeshworkInternal medicinemedicineAnimalsReceptorCells CulturedEndothelin-1ChemistryCloprostenolMuscle SmoothEndothelin 1medicine.anatomical_structureProstaglandin F2alphaEndocrinologyCalciumCattleTrabecular meshworkFura-2Endothelin receptorMuscle Contractionmedicine.drugInvestigative Opthalmology & Visual Science
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Tiburones de aguas profundas en el Mioceno medio de Alicante: implicaciones para la recuperación de los ecosistemas abisales mediterráneos después de…

2017

El estudio de la localidad clásica de El Chorrillo del Mioceno Medio (Serravaliense) en la Provincia de Alicante, ha permitido la recuperación de una interesante asociación de microrestos de tiburones caracterizada por al menos siete taxones (Deania calcea, ¿Isistius triangulus, ¿Squaliolus cf. schaubi, ¿Paraetmopterus sp., Pristiophorus sp., Scyliorhinus sp. y un Squaliforme indeterminado) pertenecientes a tres órdenes diferentes (Squaliformes, Pristiophoriformes y Carcharhiniformes). Asociados a estos microrestos se han identificado también macrodientes pertenecientes a Cosmopolitodus hastalis, Isurus sp., Hemipristis serra, Odontaspis sp., Carcharhinus spp. y ¿Otodus (Megaselachus) megal…

AlicanteMediterráneo OccidentalElasmobranquiosAlicante Western MediterraneanMiocenoFauna abisalesMiocenePaleontologiaAbysal faunasPaleontologíaElasmobranchiiEvolució (Biologia)
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The earliest tupilakosaurid amphibian with diplospondylous vertebrae from the Late Permian of southern France

2007

5 pages; International audience; A well-preserved vertebral column from the Late Permian of Southern France (Lopingian, La Lieude Formation, Lodève Basin) is described. It is composed of diplospondylous vertebrae and is most comparable with the temnospondyl Tupilakosaurus previously known from the Early Triassic of Greenland and Russia. This new specimen therefore represents the earliest occurrence of a diplospondylous tupilakosaur, and extends the geographic range of the group to Western Europe. It is an aquatic temnospondyl that used the anguilliform undulatory mode of swimming.

AmphibianTupilakosaurusbiologyPermianEarly TriassicPaleontologyStructural basinbiology.organism_classificationPaleontologyGroup (stratigraphy)biology.animalWestern europe[SDU.STU.PG]Sciences of the Universe [physics]/Earth Sciences/PaleontologyGeology[ SDU.STU.PG ] Sciences of the Universe [physics]/Earth Sciences/PaleontologyJournal of Vertebrate Paleontology
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The submerged syntax between Late Antiquity and the Modern Age. Sources, models, and interpretative strategies

2021

The present volume contains selected papers from the international conference on “The submerged syntax between Late Antiquity and the Modern Age. Sources, models, and interpretative strategies”, that took place in Palermo, 28-29 November 2019, hosted by the Department of Humanities at the University of Palermo. The conference was organized under the umbrella of a project of national relevance funded by the Italian Ministry of Education, University and Research, named “Parts of speech meet rhetorics: searching for syntax in the continuity between the Middle Ages and the Modern Age” (PRIN 20172F2FEZ, March 2017).

Ancient and Medieval GrammariansWestern theory of SyntaxSettore L-LIN/01 - Glottologia E Linguistica
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The lipoprotein receptor LRP1 modulates sphingosine-1-phosphate signaling and is essential for vascular development

2014

Low density lipoprotein receptor-related protein 1 (LRP1) is indispensable for embryonic development. Comparing different genetically engineered mouse models, we found that expression of Lrp1 is essential in the embryo proper. Loss of LRP1 leads to lethal vascular defects with lack of proper investment with mural cells of both large and small vessels. We further demonstrate that LRP1 modulates Gi-dependent sphingosine-1-phosphate (S1P) signaling and integrates S1P and PDGF-BB signaling pathways, which are both crucial for mural cell recruitment, via its intracellular domain. Loss of LRP1 leads to a lack of S1P-dependent inhibition of RAC1 and loss of constraint of PDGF-BB-induced cell migra…

AngiogenesisBlotting WesternBecaplerminEmbryonic DevelopmentNeovascularization PhysiologicRAC1BiologyReal-Time Polymerase Chain ReactionMural cellchemistry.chemical_compoundMiceCell MovementSphingosineHuman Umbilical Vein Endothelial CellsAnimalsHumansSphingosine-1-phosphateMolecular BiologyResearch ArticlesIn Situ HybridizationSphingosineTumor Suppressor ProteinsCell migrationCell BiologyProto-Oncogene Proteins c-sisLRP1ImmunohistochemistryCell biologyMicroscopy ElectronchemistryReceptors LDLLow-density lipoproteinSignal transductionLysophospholipidsGenetic EngineeringLow Density Lipoprotein Receptor-Related Protein-1Developmental BiologySignal Transduction
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