Search results for "Wnt"

SYSCILIA, “A systems biology approach to dissect cilia function and its disruption in human genetic disease”

2012

Primary cilia are basically signaling hubs, harboring amongst others the noncanonical WNT, Hedgehog,and PDGF signaling systems, and their disruption leads to striking developmental defects. Some ciliopathy-associated proteins have recently been revealed to be physically or functionally associated in several distinct groupings, with limited connections to other crucial biological processes. Early proteomics studies have also suggested a discrete repertoire of about 1000 proteins within the organelle (i.e. <5% of the proteome) that are still in need of organisation into pathways and networks. Small, relatively isolated systems are often targeted by systems biology approaches under the assumpt…

Hypoxia-inducible factor 1Α may regulate the commitment of mesenchymal stromal cells toward angio-osteogenesis by mirna-675-5P

2017

Abstract Background aims During bone formation, angiogenesis and osteogenesis are regulated by hypoxia, which is able to induce blood vessel formation, as well as recruit and differentiate human mesenchymal stromal cells (hMSCs). The molecular mechanisms involved in HIF-1α response and hMSC differentiation during bone formation are still unclear. This study aimed to investigate the synergistic role of hypoxia and hypoxia-mimetic microRNA miR-675-5p in angiogenesis response and osteo-chondroblast commitment of hMSCs. Methods By using a suitable in vitro cell model of hMSCs (maintained in hypoxia or normoxia), the role of HIF-1α and miR-675-5p in angiogenesis and osteogenesis coupling was inv…

MMP-10/stromelysin-2 promotes invasion of head and neck cancer.

2011

BackgroundPeriostin, IFN-induced transmembrane protein 1 (IFITM1) and Wingless-type MMTV integration site family, member 5B (Wnt-5b) were previously identified as the invasion promoted genes of head and neck squamous cell carcinoma (HNSCC) by comparing the gene expression profiles between parent and a highly invasive clone. We have previously reported that Periostin and IFITM1 promoted the invasion of HNSCC cells. Here we demonstrated that Wnt-5b overexpression promoted the invasion of HNSCC cells. Moreover, stromelysin-2 (matrix metalloproteinase-10; MMP-10) was identified as a common up-regulated gene among Periostin, IFITM1 and Wnt-5b overexpressing HNSCC cells by using microarray data s…

Targeting cancer stem cells and the tumor microenvironment

2015

Compelling evidence indicates that the survival and behavior of cancer stem cells (CSCs) are positively regulated by specific stimuli received from the tumor microenvironment, which dictates the maintenance of stemness, invasiveness, and protection against drug-induced apoptotic signals. CSCs are per se endowed with multiple treatment resistance capabilities, thus the eradication of CSC pools offers a precious strategy in achieving a long-term cancer remission. Numerous therapies, aimed at eradicating CSCs, have been elaborated such as: (i) selective targeting of CSCs, (ii) modulating their stemness and (iii) influencing the microenvironment. In this context, markers commonly exploited to i…

Progression of colorectal cancers correlates with overexpression and loss of polarization of expression of the htid-1 tumor suppressor.

2007

Recently, we identified htid-1, the human counterpart of the Drosophila tumor suppressor gene lethal(2)tumorous imaginal discs [l(2)tid], as a direct molecular ligand of the adenomatous polyposis coli (APC) tumor suppressor. The gene encodes three cytosolic (Tid50, Tid48 and Tid46) and three mitochondrial (Tid43, Tid40 and Tid38) proteins. In the colorectal epithelium the cytosolic forms hTid50/hTid48 interact under physiological conditions with the N-terminal region of APC. This complex which associates with additional proteins such as Hsp70, Hsc70, Actin, Dvl and Axin defines a novel physiological state of APC unrelated to beta-catenin degradation. Here we show that the expression of the …

Relevance of the macrophage phenotype in mucosal regeneration in Inflammatory Bowel Disease

2015

La enfermedad Inflamatoria Intestinal (EII) es una enfermedad crónica, recidivante, de carácter sistémico que afecta principalmente al tracto gastrointestinal. Esta patología engloba dos entidades clínicas, la Enfermedad de Crohn (EC) y la Colitis Ulcerosa (CU), que difieren en algunos aspectos pero comparten la disrupción de la barrera epitelial y la respuesta exacerbada del sistema inmunológico a la flora bacteriana. La etiología de la enfermedad es desconocida pero se sabe que factores genéticos (tanto alteraciones a nivel genético como la presencia de miRNAs), ambientales (tales como la dieta, tabaco, actividad física o la calidad del sueño), flora intestinal y sistema inmunológico cont…

Methodology for the estimation of the increase in time loss due to future increase in tropical cyclone intensity in Japan

2009

Published version of an article from the journal: Climatic Change. The original publication is available at Spingerlink. http://dx.doi.org/10.1007/s10584-009-9725-9 The present paper develops a methodology for estimating the risks and consequences of possible future increases in tropical cyclone intensities that would allow policy makers to relatively quickly evaluate the cost of different mitigation strategies. The methodology simulates future tropical cyclones by modifying the intensity of historical tropical cyclones between the years 1978 and 2007. It then uses a Monte Carlo Simulation to obtain the expected number of hours that a certain area can expect to be affected by winds of a giv…

Rib-Roller Wear in Tapered Rolling Element Bearings: Analysis and Development of Test Rig for Condition Monitoring

2020

Rolling Element Bearings (REBs) are present in virtually all machines with moving or rotating parts, and are vital for proper performance and safe operation. Condition Monitoring (CM) of bearings often receive particular interest, as this component group rarely reach design lifetime and hence is responsible for unplanned machine downtime. Unplanned maintenance can represent a large cost which motivates development of improved CM methods for implementation of advanced maintenance regimes. Based on observations of a used bearing from an offshore drilling machine, wear on roller ends in the rib-roller contact area was identified as an area of interest for future research. A test rig for creati…

Wnt/β-Catenin dependent roles of the Pygopus family of nuclear factors during Zebrafish early dorso-ventral specification

2014

Pharmacogenomic identification of small molecules for lineage specific manipulation of subventricular zone germinal activity

2017

Strategies for promoting neural regeneration are hindered by the difficulty of manipulating desired neural fates in the brain without complex genetic methods. The subventricular zone (SVZ) is the largest germinal zone of the forebrain and is responsible for the lifelong generation of interneuron subtypes and oligodendrocytes. Here, we have performed a bioinformatics analysis of the transcriptome of dorsal and lateral SVZ in early postnatal mice, including neural stem cells (NSCs) and their immediate progenies, which generate distinct neural lineages. We identified multiple signaling pathways that trigger distinct downstream transcriptional networks to regulate the diversity of neural cells …