Search results for "XB"
showing 10 items of 78 documents
Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons
2014
Direct lineage-reprogramming of non-neuronal cells into induced neurons (iNs) may provide insights into the molecular mechanisms underlying neurogenesis and enable new strategies for in vitro modeling or repairing the diseased brain. Identifying brain-resident non-neuronal cell types amenable to direct conversion into iNs might allow for launching such an approach in situ, i.e. within the damaged brain tissue. Here we describe a protocol developed in the attempt of identifying cells derived from the adult human brain that fulfill this premise. This protocol involves: (1) the culturing of human cells from the cerebral cortex obtained from adult human brain biopsies; (2) the in vitro expansio…
ER stress in human hepatic cells treated with Efavirenz: Mitochondria again
2013
Background & Aims ER stress is associated with a growing number of liver diseases, including drug-induced hepatotoxicity. The non-nucleoside analogue reverse transcriptase inhibitor Efavirenz, a cornerstone of the multidrug strategy employed to treat HIV1 infection, has been related to the development of various adverse events, including metabolic disturbances and hepatic toxicity, the mechanisms of which remain elusive. Recent evidence has pinpointed a specific mitochondrial effect of Efavirenz in human hepatic cells. This study assesses the induction of ER stress by Efavirenz in the same model and the implication of mitochondria in this process. Methods Primary human hepatocytes and Hep3B…
Efavirenz induces interactions between leucocytes and endothelium through the activation of Mac-1 and gp150,95
2013
The potential cardiovascular (CV) toxicity associated with combined antiretroviral therapy (cART) has been attributed mainly to the nucleoside reverse transcriptase inhibitors abacavir and didanosine. However, the other two components of cART--non-nucleoside reverse transcriptase inhibitors (NNRTIs) and protease inhibitors (PIs)--may also be implicated, either directly or by influencing the action of the other drugs. This study evaluates the acute direct effects of the NNRTIs efavirenz and nevirapine and one of the most widely employed PIs, lopinavir, on leucocyte-endothelium interactions, a hallmark of CV disease.Drugs were analysed in vitro in human cells (interactions of peripheral blood…
Cyclin-dependent kinase inhibitor p21 controls adult neural stem cell expansion by regulating Sox2 gene expression.
2012
Summary In the adult brain, continual neurogenesis of olfactory neurons is sustained by the existence of neural stem cells (NSCs) in the subependymal niche. Elimination of the cyclin-dependent kinase inhibitor 1A (p21) leads to premature exhaustion of the subependymal NSC pool, suggesting a relationship between cell cycle control and long-term self-renewal, but the molecular mechanisms underlying NSC maintenance by p21 remain unexplored. Here we identify a function of p21 in the direct regulation of the expression of pluripotency factor Sox2, a key regulator of the specification and maintenance of neural progenitors. We observe that p21 directly binds a Sox2 enhancer and negatively regulate…
Sox2-Mediated Conversion of NG2 Glia into Induced Neurons in the Injured Adult Cerebral Cortex
2014
Summary The adult cerebral cortex lacks the capacity to replace degenerated neurons following traumatic injury. Conversion of nonneuronal cells into induced neurons has been proposed as an innovative strategy toward brain repair. Here, we show that retrovirus-mediated expression of the transcription factors Sox2 and Ascl1, but strikingly also Sox2 alone, can induce the conversion of genetically fate-mapped NG2 glia into induced doublecortin (DCX)+ neurons in the adult mouse cerebral cortex following stab wound injury in vivo. In contrast, lentiviral expression of Sox2 in the unlesioned cortex failed to convert oligodendroglial and astroglial cells into DCX+ cells. Neurons induced following …
CSS Grid Layout pielietošana tīmekļa vietnes izstrādē
2018
Režģa izkārtojuma ieviešanai tīmekļa vietnēs līdz šim ir izmantoti elementi, kas nav bijuši paredzēti šim mērķim, tādēļ globālā tīmekļa konsorcijs ir izstrādājis standartu priekš režģa izkārtojuma ieviešanas tīmekļa vietnēs – CSS Grid Layout. Bakalaura darba mērķis ir sagatavot un analizēt teorētisko materiālu bāzi par tīmekļa vietņu dizaina ieviešanas metodēm un to vēsturi, uz ko balstoties izpētīt CSS Grid Layout ieviešanas iespējas tīmekļa vietņu izstrādes procesā.
Consensus guidelines for the detection of immunogenic cell death
2014
Apoptotic cells have long been considered as intrinsically tolerogenic or unable to elicit immune responses specific for dead cell-associated antigens. However, multiple stimuli can trigger a functionally peculiar type of apoptotic demise that does not go unnoticed by the adaptive arm of the immune system, which we named "immunogenic cell death" (ICD). ICD is preceded or accompanied by the emission of a series of immunostimulatory damage-associated molecular patterns (DAMPs) in a precise spatiotemporal configuration. Several anticancer agents that have been successfully employed in the clinic for decades, including various chemotherapeutics and radiotherapy, can elicit ICD. Moreover, defect…
Signature of the presence of a third body orbiting around XB 1916-053
2015
The ultra-compact dipping source \object{XB 1916-053} has an orbital period of close to 50 min and a companion star with a very low mass (less than 0.1 M$_{\odot}$). The orbital period derivative of the source was estimated to be $1.5(3) \times 10^{-11}$ s/s through analysing the delays associated with the dip arrival times obtained from observations spanning 25 years, from 1978 to 2002. The known orbital period derivative is extremely large and can be explained by invoking an extreme, non-conservative mass transfer rate that is not easily justifiable. We extended the analysed data from 1978 to 2014, by spanning 37 years, to verify whether a larger sample of data can be fitted with a quadra…
X-ray spectroscopy of MXB 1728-34 with XMM-Newton
2011
We have analysed an XMM-Newton observation of the low mass X-ray binary and atoll source MXB 1728-34. The source was in a low luminosity state during the XMM-Newton observation, corresponding to a bolometric X-ray luminosity of 5*10E36 d^2 erg/s, where d is the distance in units of 5.1 kpc. The 1-11 keV X-ray spectrum of the source, obtained combining data from all the five instruments on-board XMM-Newton, is well fitted by a Comptonized continuum. Evident residuals are present at 6-7 keV which are ascribed to the presence of a broad iron emission line. This feature can be equally well fitted by a relativistically smeared line or by a self-consistent, relativistically smeared, reflection mo…
Broadband spectral analysis of MXB 1659-298 in its soft and hard state
2019
The X-ray transient eclipsing source MXB 1659-298 went in outburst in 1999 and 2015, respectively, during which it was observed by XMM-Newton, NuSTAR and Swift. Using these observations we studied the broadband spectrum of the source to constrain the continuum components and to verify the presence of a reflection component. We analysed the soft and hard state of the source, finding that the soft state can be modelled with a thermal component associated with the inner accretion disc plus a Comptonised component. A smeared reflection component and the presence of an ionised absorber are also requested in the best-fit model. On the other hand, the direct continuum emission in the hard state ca…