Search results for "YKI"
showing 10 items of 414 documents
Two distinct Ca2+ influx pathways activated by the bradykinin B2 receptor.
1996
The hormone-induced depletion of cellular Ca stores provides a signal for the Ca2+ influx into electrically non-excitable cells; however, the underlying molecular mechanisms remain elusive. Therefore, we analyzed bradykinin-activated Ca2+ influx into human foreskin fibroblast cells, HF-15, by fura-2 and 45Ca labeling to discriminate between Ca2+ influx into the fura-sensitive compartment and Ca uptake into fura-insensitive Ca stores. Bradykinin-activated CaZt influx into the fura-sensitive compartment was blocked by inhibitors of NO synthases. These inhibitors also suppressed bradykinin-activated increases in cGMP, indicating that the NO-dependent increase in cGMP is involved in the activat…
Ligand-induced phosphorylation/dephosphorylation of the endogenous bradykinin B2 receptor from human fibroblasts.
1996
We have studied the ligand-induced phosphorylation/dephosphorylation of the bradykinin B2 receptor endogenously expressed in human HF-15 fibroblasts. An antiserum (AS346) to a synthetic peptide (CRS36), derived from the extreme carboxyl terminus of the human B2 receptor, precipitated the receptor from solubilized membranes of HF-15 cells that had been labeled with [32P]orthophosphate. A low basal level of B2 receptor phosphorylation was found in the absence of a ligand. Stimulation of the cells with the B2 receptor agonists bradykinin, [Lys0,Hyp3]bradykinin, kallidin, and T-kinin resulted in a rapid and efficient phosphorylation of the receptor. The B2 receptor antagonist HOE140 and the B1 …
The N-terminal Amino Group of [Tyr8]Bradykinin Is Bound Adjacent to Analogous Amino Acids of the Human and Rat B2 Receptor
1996
To obtain data of the bradykinin B2 receptor's agonist binding site, we used a combined approach of affinity labeling and "immunoidentification" of receptor fragments generated by cyanogen bromide cleavage. Domain-specific antibodies to the various extracellular receptor domains were applied to detect receptor fragments with covalently attached [125I-Tyr8]bradykinin. As a cross-linker we used the homobifunctional reagent disuccinimidyl tartarate (DST), which reacts preferentially with primary amines. With this technique a [125I-Tyr8]bradykinin-labeled receptor fragment derived from the third extracellular domain was identified. The epsilon-amino group of lysine (Lys172) of the human B2 rece…
Premeditated automaticity : the role of explicit cognition in the development of obsessive smoking
2010
- Abstract The purpose of the present study was to examine the role of explicit cognitions in relation to the development of obsessive smoking passion. Data derives from a longitudinal internet survey conducted among 939 daily smokers over a period of four months. Mental representations were the strongest predictor of obsessive smoking passion (ß=.47, p<.001) followed by habit strength (ß=.25, p<.001) and number of cigarettes smoked (ß=.09, p<.01). Altogether the variables accounted for 48% of the variance in obsessive smoking passion. The results indicated that mental representations, along with automatic processes, play an important role in the prediction of obsessive smoking passion. The…
Short-Time Ocular Ischemia Induces Vascular Endothelial Dysfunction and Ganglion Cell Loss in the Pig Retina
2019
Visual impairment and blindness are often caused by retinal ischemia-reperfusion (I/R) injury. We aimed to characterize a new model of I/R in pigs, in which the intraocular pathways were not manipulated by invasive methods on the ocular system. After 12 min of ischemia followed by 20 h of reperfusion, reactivity of retinal arterioles was measured in vitro by video microscopy. Dihydroethidium (DHE) staining, qPCR, immunohistochemistry, quantification of neurons in the retinal ganglion cell layer, and histological examination was performed. Retinal arterioles of I/R-treated pigs displayed marked attenuation in response to the endothelium-dependent vasodilator, bradykinin, compared to sham-tre…
Gravides røykevaner i Aust-Agder : rapport fra et prosjekt i regi av Høgskolen i Agder, på oppdrag av Fylkesmannen i Aust-Agder
2006
"Prosjektets varighet: 1/1-2004 - 31/12-2004" Målgruppe for surveyundersøkelsen var samtlige kvinner hjemmehørende i Aust-Agder som fødte fra 1/1-04 tom 31/12-2004. Det ble det utlevert 851 spørreskjemaer. 734 svar innkom, dvs. en svarprosent på 86,3 %. 241 kvinner (32,8 %) bekreftet at de røkte ved svangerskapets start. Undersøkelsen viste at hvorvidt kvinnene definerte seg som røykere i stor grad var avhengig av hvordan spørsmål om røykevaner ble stilt. Alle grader av røyking inkludert angav 200 (27,2 %) av kvinnene at de røkte etter fødsel. Røykenedgang i løpet av svangerskapet i forhold til totalutvalget var altså på 5,6 %. Det vil si at 17 % av de som røkte ved svangerskapets start slu…
What to Expect and What to Focus on in SQL Query Teaching
2019
In the process of learning a new computer language, writing erroneous statements is part of the learning experience. However, some errors persist throughout the query writing process and are never corrected. Structured Query Language (SQL) consists of a number of different concepts such as expressions, joins, grouping and ordering, all of which by nature invite different possible errors in the query writing process. Furthermore, some of these errors are relatively easy for a student to fix when compared to others. Using a data set from three student cohorts with the total of 744 students, we set out to explore which types of errors are persistent, i.e., more likely to be left uncorrected by…
SQL Education
2020
Structured Query Language (SQL) skills are crucial in software engineering and computer science. However, teaching SQL effectively requires both pedagogical skill and considerable knowledge of the language. Educators and scholars have proposed numerous considerations for the betterment of SQL education, yet these considerations may be too numerous and scattered among different fora for educators to find and internalize, as no systematic mappings or literature reviews regarding SQL education have been conducted. The two main goals of this mapping study are to provide an overview of educational SQL research topics, research types, and publication fora, and to collect and propagate SQL teachi…
The Effects of Database Complexity on SQL Query Formulation
2020
In Structured Query Language (SQL) education, students often execute queries against a simple exercise database. Recently, databases that are more realistic have been utilized to the effect that students find exercises more interesting and useful, as these databases more accurately mimic databases students are likely to encounter in their future work environments. However, using even the most engaging database can be counterproductive to learning, if a student is not able to formulate correct queries due to the complexity of the database schema. Scientific evidence on the effects of database complexity on student’s query formulation is limited, and with queries from 744 students against thr…
Jukolan veljekset maallikkopsykiatrin vastaanotolla : ADHD-nimike ja muut tarttuvat diagnoosit Seitsemän veljeksen nykyluennassa
2022
Helsingin Sanomien julkaisemassa tietokirjassa Seitsemän miestä (2005) esitetään psykiatrisia diagnooseja Aleksis Kiven Seitsemässä veljeksessä (1870) kuvatuista henkilöistä. Tällainen diagnosoimisen tapa osallistuu kulttuurin diagnostisoitumisen prosessiin – siihen, että spesifit diagnoosinimikkeet saavat yhä keskeisemmän sijan kulttuurissa. Analysoin teokseen kirjattuja diagnosointeja kulttuurisen mielenterveystutkimuksen näkökulmasta. In this article I analyze how psychiatric diagnoses are used in the book Seitsemän miestä (The Seven Men, 2005), a nonfictional work by journalist Ilkka Malmberg. The book is published by Helsingin Sanomat, the largest subscription news-paper in Finland, an…