Search results for "Yeasts"

showing 10 items of 151 documents

Misinterpretation risks of global stochastic optimisation of kinetic models revealed by multiple optimisation runs

2016

Abstract One of use cases for metabolic network optimisation of biotechnologically applied microorganisms is the in silico design of new strains with an improved distribution of metabolic fluxes. Global stochastic optimisation methods (genetic algorithms, evolutionary programing, particle swarm and others) can optimise complicated nonlinear kinetic models and are friendly for unexperienced user: they can return optimisation results with default method settings (population size, number of generations and others) and without adaptation of the model. Drawbacks of these methods (stochastic behaviour, undefined duration of optimisation, possible stagnation and no guaranty of reaching optima) cau…

Statistics and ProbabilitySucroseMathematical optimizationComputer scienceSystems biology0206 medical engineeringMetabolic network02 engineering and technologyModels BiologicalGeneral Biochemistry Genetics and Molecular Biology03 medical and health sciencesYeastsConvergence (routing)HomeostasisUse caseLimit (mathematics)030304 developmental biologyStochastic Processes0303 health sciencesGeneral Immunology and MicrobiologyApplied MathematicsParticle swarm optimizationGeneral MedicineEnzymesSaccharumConstraint (information theory)Nonlinear systemModeling and SimulationGeneral Agricultural and Biological SciencesMetabolic Networks and Pathways020602 bioinformaticsMathematical Biosciences
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Eukaryotic tRNAs(Pro): primary structure of the anticodon loop; presence of 5-carbamoylmethyluridine or inosine as the first nucleoside of the antico…

1990

The modified nucleoside U*, located in the first position of the anticodon of yeast, chicken liver and bovine liver tRNA(Pro) (anticodon U*GG), has been determined by means of TLC, HPLC, ultraviolet spectrum and gas chromatography-mass spectrometry. The structure was established as 5-carbamoylmethyluridine (ncm5U). In addition, we report on the primary structures of the above-mentioned tRNAs as well as those which have the IGG anticodon. In yeast, the two tRNA(Pro) (anticodons U*GG and IGG) differ by eight nucleotides, whereas in chicken and in bovine liver, both anticodons are carried by the same 'body tRNA' with one posttranscriptional exception at position 32, where pseudouridine is asso…

StereochemistryMolecular Sequence DataBiophysicsBiologyBiochemistryPseudouridinechemistry.chemical_compoundRNA Transfer ProRNA TransferStructural BiologyYeastsGeneticsmedicineAnticodonAnimalsNucleotideInosineUridinechemistry.chemical_classificationChromatographyBase SequenceMolecular StructureProtein primary structureFungal geneticsRNARNA FungalRNA Transfer Amino Acid-SpecificInosinechemistryBiochemistryTransfer RNANucleic Acid ConformationCattleSpectrophotometry UltravioletNucleosideChickensmedicine.drugBiochimica et biophysica acta
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Chromatin modifiers and recombination factors promote a telomere fold-back structure, that is lost during replicative senescence.

2020

Telomeres have the ability to adopt a lariat conformation and hence, engage in long and short distance intra-chromosome interactions. Budding yeast telomeres were proposed to fold back into subtelomeric regions, but a robust assay to quantitatively characterize this structure has been lacking. Therefore, it is not well understood how the interactions between telomeres and non-telomeric regions are established and regulated. We employ a telomere chromosome conformation capture (Telo-3C) approach to directly analyze telomere folding and its maintenance in S. cerevisiae. We identify the histone modifiers Sir2, Sin3 and Set2 as critical regulators for telomere folding, which suggests that a dis…

TelomeraseProtein Folding:Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::DNA-Binding Proteins::Rad52 DNA Repair and Recombination Protein [Medical Subject Headings]:Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Fungal Proteins::Saccharomyces cerevisiae Proteins [Medical Subject Headings]Gene ExpressionYeast and Fungal ModelsArtificial Gene Amplification and ExtensionQH426-470BiochemistryPolymerase Chain ReactionChromosome conformation captureHistonesCromatina0302 clinical medicineSirtuin 2Macromolecular Structure AnalysisSilent Information Regulator Proteins Saccharomyces cerevisiaeCellular Senescence:Organisms::Eukaryota::Fungi::Yeasts::Saccharomyces::Saccharomyces cerevisiae [Medical Subject Headings]0303 health sciencesChromosome BiologyEukaryota:Phenomena and Processes::Genetic Phenomena::Genetic Processes::DNA Replication [Medical Subject Headings]TelomereSubtelomere:Anatomy::Cells::Cellular Structures::Intracellular Space::Cell Nucleus::Cell Nucleus Structures::Intranuclear Space::Chromosomes::Chromosome Structures::Telomere [Medical Subject Headings]Chromatin3. Good healthChromatinCell biologyNucleic acidsTelomeres:Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Cycle::Cell Division::Telomere Homeostasis [Medical Subject Headings]Experimental Organism SystemsDaño del ADNEpigeneticsResearch ArticleSenescenceDNA Replication:Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Amidohydrolases::Histone Deacetylases [Medical Subject Headings]Chromosome Structure and FunctionProtein StructureSaccharomyces cerevisiae ProteinsSaccharomyces cerevisiaeBiologyResearch and Analysis MethodsHistone DeacetylasesChromosomes03 medical and health sciencesSaccharomycesModel Organisms:Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Transferases::One-Carbon Group Transferases::Methyltransferases [Medical Subject Headings]:Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Intracellular Signaling Peptides and Proteins::Sirtuins::Sirtuin 2 [Medical Subject Headings]:Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Fungal Proteins::Saccharomyces cerevisiae Proteins::Silent Information Regulator Proteins Saccharomyces cerevisiae [Medical Subject Headings]DNA-binding proteinsGenetics:Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Recombinases::Rec A Recombinases::Rad51 Recombinase [Medical Subject Headings]Molecular Biology TechniquesMolecular Biology030304 developmental biologyCromosomasSenescencia celularOrganismsFungiBiology and Life SciencesProteinsTelomere HomeostasisCell BiologyDNAMethyltransferasesG2-M DNA damage checkpointProteína recombinante y reparadora de ADN Rad52YeastTelomereRad52 DNA Repair and Recombination ProteinRepressor ProteinsAnimal Studies:Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Transcription Factors::Repressor Proteins [Medical Subject Headings]DNA damageRad51 RecombinaseHomologous recombination030217 neurology & neurosurgeryTelómeroDNA DamagePLoS Genetics
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Microbial dynamics in durum wheat kernels during aging

2020

In the present work the microbial dynamics in wheat kernels were evaluated over time. The main aim of this research was to study the resistance of lactic acid bacteria (LAB) and yeasts associated to unprocessed cereals used for bread making during long term conservation. To this purpose four Triticum durum Desf. genotypes including two modern varieties (Claudio and Simeto) and two Sicilian wheat landraces (Russello and Timilia) were analysed by a combined culture-independent and -dependent microbiological approach after one, two or three years from cultivation and threshing. DNA based MiSeq Illumina technology was applied to reveal the entire bacterial composition of all semolina samples. T…

Time FactorsGenotypeLactobacillus pentosusMicrobiology03 medical and health sciencesLactobacillusYeastsDurum wheat kernelsLactic acid bacteriaFood scienceDurum wheat kernelTriticum030304 developmental biology0303 health sciencesbiologyBacteria030306 microbiologyLactobacillus brevisMicrobiotaMicrobial persistencefood and beveragesGeneral MedicineBreadbiology.organism_classificationEnterococcus duransSettore AGR/02 - Agronomia E Coltivazioni ErbaceeSettore AGR/15 - SCIENZE E TECNOLOGIE ALIMENTARIEnterococcusFood StorageSourdoughMiSeq IlluminaFood MicrobiologyPediococcusStenotrophomonasEdible GrainFood ScienceEnterococcus faeciumSettore AGR/16 - Microbiologia Agraria
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Fermentation profiles of Manzanilla-Aloreña cracked green table olives in different chloride salt mixtures.

2010

NaCl plays an important role in table olive processing affecting the flavour and microbiological stability of the final product. However, consumers demand foods low in sodium, which makes necessary to decrease levels of this mineral in fruits. In this work, the effects of diverse mixtures of NaCl, CaCl2 and KCl on the fermentation profiles of cracked directly brined Manzanilla-Aloreña olives, were studied by means of response surface methodology based in a simplex lattice mixture design with constrains. All salt combinations led to lactic acid processes. The growth of Enterobacteriaceae populations was always limited and partially inhibited by the presence of CaCl2. Only time to reach half …

Time FactorsWater activityFood HandlingTable oliveSodium chlorideSodiumPopulationMicroorganismsColony Count Microbialchemistry.chemical_elementBacterial growthSodium ChlorideMicrobiologyFermentation profileCalcium chloridePotassium Chloridechemistry.chemical_compoundCalcium ChlorideBriningEnterobacteriaceaeSpecies SpecificityFood PreservationOleaYeastsFood scienceeducationeducation.field_of_studyDose-Response Relationship DrugWaterHydrogen-Ion ConcentrationYeastLactic acidKineticschemistryBiochemistryPotassium chlorideTasteFermentationFood MicrobiologyFermentationSaltsFood ScienceFood microbiology
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A genomic view of mRNA turnover in yeast

2011

The steady-state mRNA level is the result of two opposing processes: transcription and degradation; both of which can provide important points to regulate gene expression. In the model organism yeast Saccharomyces cerevisiae, it is now possible to determine, at the genomic level, the transcription and degradation rates, as well as the mRNA amount, using DNA chip or parallel sequencing technologies. In this way, the contribution of both rates to individual and global gene expressions can be analysed. Here we review the techniques used for the genomic evaluation of the transcription and degradation rates developed for this yeast, and we discuss the integration of the data obtained to fully an…

Transcription Geneticved/biology.organism_classification_rank.speciesSaccharomyces cerevisiaeSaccharomyces cerevisiaeComputational biologyGeneral Biochemistry Genetics and Molecular BiologyTranscripció genèticaStress PhysiologicalTranscription (biology)YeastsGene expressionRNA MessengerModel organismGeneGeneticsMassive parallel sequencingGeneral Immunology and Microbiologybiologyved/biologyRNA FungalGenomicsGeneral Medicinebiology.organism_classificationYeastGenòmicaRNAGenome FungalDNA microarrayTranscriptomeGeneral Agricultural and Biological SciencesComptes Rendus Biologies
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Sus1, a functional component of the SAGA histone acetylase complex and the nuclear pore-associated mRNA export machinery

2004

12 páginas, 7 figuras, 1 tabla. Material suplementario en: https://doi.org/10.1016/S0092-8674(03)01025-0. The SUS1 sequences have been deposited in GenBank with the accession number AY278445.

Transcriptional ActivationNucleocytoplasmic Transport ProteinsDNA ComplementarySaccharomyces cerevisiae ProteinsMolecular Sequence DataActive Transport Cell NucleusPorinsRNA polymerase IIBiologyGeneral Biochemistry Genetics and Molecular BiologyFungal ProteinsTranscription (biology)AcetyltransferasesGene Expression Regulation FungalYeastsGene expressionGenes RegulatorTranscriptional regulationAmino Acid SequenceRNA MessengerNuclear proteinPromoter Regions GeneticHistone AcetyltransferasesRegulation of gene expressionCell NucleusBase SequenceBiochemistry Genetics and Molecular Biology(all)Nuclear ProteinsRNA-Binding ProteinsMolecular biologyCell biologySAGA complexRibonucleoproteinsbiology.proteinNuclear PoreGenes LethalChromatin immunoprecipitation
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Yeast biota of naturally fermented black olives in different brines made from cv. Gemlik grown in various districts of the Cukurova region of Turkey.

2015

In this study, the yeast microbiota of naturally fermented black olives made from cv. Gemlik, grown in three different districts of the Cukurova region of Turkey, were investigated. Fermentations were conducted for 180days in three different brines, including NaCl 10% w/v, NaCl 8% w/v and NaCl 8% w/v added with glucose 0.5%. In total, 223 yeasts were isolated and then identified by PCR-RFLP analysis of the 5.8S ITS rRNA region and sequence information for the D1/D2 domains of the 26S rRNA gene. A broad range of yeast biodiversity was identified, including eight genera and nine species. Candida boidinii (41%), Wickerhamomyces anomalus (32%) and Saccharomyces sp. (18%) were predominant yeasts…

TurkeyMicrobiotaGemlik; molecular; NaCl; PCR; table olive; yeast; Biochemistry; Biotechnology; Genetics; Bioengineering; Applied Microbiology and BiotechnologyGemlikBioengineeringBiodiversityyeastBiochemistryApplied Microbiology and BiotechnologyPCRNaClGeneticOleaYeastsFermentationmoleculartable oliveBiotechnologySettore AGR/16 - Microbiologia AgrariaYeast (Chichester, England)
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Interspecific hybridization and aneuploidy as adaptive mechanisms in saccharomyces yeasts

2019

Doctorado en Biomedicina y Biotecnología.

UNESCO::CIENCIAS DE LA VIDAgenome:CIENCIAS DE LA VIDA [UNESCO]saccharomyces yeasts
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Study of glycerol and respiro-fermentative metabolism diversity among Saccharomyces yeasts

2016

Introducción Las levaduras son responsables de numerosos procesos biotecnológicos tales como la producción de bebidas y alimentos fermentados. Las levaduras de interés biotecnológico son organismos muy especializados que han evolucionado bajo restrictivas condiciones ambientales en distintos ambientes manipulados por el hombre. Durante el proceso de adaptación a estos ambientes manipulados por el hombre, distintas cepas y especies del género Saccharomyces se han visto sometidas a procesos selectivos generados por su uso inconsciente en la fermentación alcohólica (Querol et al., 2003), lo que también ha generado diferencias adaptativas entre ellas (Barrio et al., 2006). Aunque la especie más…

UNESCO::CIENCIAS DE LA VIDAyeastsglycerolrespiro-fermentative metabolism:CIENCIAS DE LA VIDA [UNESCO]
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