6533b85cfe1ef96bd12bc121

RESEARCH PRODUCT

Eukaryotic tRNAs(Pro): primary structure of the anticodon loop; presence of 5-carbamoylmethyluridine or inosine as the first nucleoside of the anticodon.

Pascale PochartThérèse HeymanKenneth C. KuoJean DesgresGérard KeithCharles W. Gehrke

subject

StereochemistryMolecular Sequence DataBiophysicsBiologyBiochemistryPseudouridinechemistry.chemical_compoundRNA Transfer ProRNA TransferStructural BiologyYeastsGeneticsmedicineAnticodonAnimalsNucleotideInosineUridinechemistry.chemical_classificationChromatographyBase SequenceMolecular StructureProtein primary structureFungal geneticsRNARNA FungalRNA Transfer Amino Acid-SpecificInosinechemistryBiochemistryTransfer RNANucleic Acid ConformationCattleSpectrophotometry UltravioletNucleosideChickensmedicine.drug

description

The modified nucleoside U*, located in the first position of the anticodon of yeast, chicken liver and bovine liver tRNA(Pro) (anticodon U*GG), has been determined by means of TLC, HPLC, ultraviolet spectrum and gas chromatography-mass spectrometry. The structure was established as 5-carbamoylmethyluridine (ncm5U). In addition, we report on the primary structures of the above-mentioned tRNAs as well as those which have the IGG anticodon. In yeast, the two tRNA(Pro) (anticodons U*GG and IGG) differ by eight nucleotides, whereas in chicken and in bovine liver, both anticodons are carried by the same 'body tRNA' with one posttranscriptional exception at position 32, where pseudouridine is associated with ncm5U (position 34) in tRNA(Pro) (U*GG) and 2'-O-methylpseudouridine is associated with inosine (position 34) in tRNA(Pro) (IGG).

yearjournalcountryeditionlanguage
1990-07-30Biochimica et biophysica acta
10.1016/0167-4781(90)90095-jhttps://pubmed.ncbi.nlm.nih.gov/2383584