Search results for "ZOL"

Intracellular route of canine parvovirus entry.

1998

ABSTRACT The present study was designed to investigate the endocytic pathway involved in canine parvovirus (CPV) infection. Reduced temperature (18°C) or the microtubule-depolymerizing drug nocodazole was found to inhibit productive infection of canine A72 cells by CPV and caused CPV to be retained in cytoplasmic vesicles as indicated by immunofluorescence microscopy. Consistent with previously published results, these data indicate that CPV enters a host cell via an endocytic route and further suggest that microtubule-dependent delivery of CPV to late endosomes is required for productive infection. Cytoplasmic microinjection of CPV particles was used to circumvent the endocytosis and membr…

Efficient killing of human colon cancer stem cells by gammadelta T lymphocytes

2009

Colon cancer comprises a small population of cancer stem cells (CSC) that is responsible for tumor maintenance and resistant to cancer therapies, possibly allowing for tumor recapitulation once treatment stops. We previously demonstrated that such chemoresistance is mediated by autocrine production of IL-4 through the up-regulation of antiapoptotic proteins. Several innate and adaptive immune effector cells allow for the recognition and destruction of cancer precursors before they constitute the tumor mass. However, cellular immune-based therapies have not been experimented yet in the population of CSCs. Here, we show that the bisphosphonate zoledronate sensitizes colon CSCs to Vgamma9Vdelt…

Combining dasatinib with dexamethasone long-term leads to maintenance of antiviral and antileukemia specific cytotoxic T cell responses in vitro

2012

Maintaining graft versus leukemia (GvL) and antivirus responses of cytotoxic T cells (CTLs) while suppressing graft-versus-host disease (GvHD) remains a challenge after allogeneic bone marrow transplantation. Clinical observations indicate that combining glucocorticoids with multi-tyrosine-kinase inhibitors could be a successful therapeutic approach. We and others have shown that the BCR-ABL/SRC kinase inhibitor dasatinib may enhance or suppress T cells in vitro. In this report, we evaluated combination effects of dasatinib and dexamethasone on CD3 + and virus-specific CD8 + T cells directly ex vivo and on antigen-specific leukemia-reactive and alloreactive CD8 + T cell clones. Functional o…

The synthesis of fluorinated heteroaromatic compounds. Part 2. Five-membered rings with two heteroatoms. A review

2007

LC-MS/MS metodes izstrāde otrās izvēles prettuberkulozes zāļu vielu un to metabolītu kvantitatīvai noteikšanai asins plazmā

2022

LC-MS/MS metodes izstrāde otrās izvēles prettuberkulozes zāļu vielu un to metabolītu kvantitatīvai noteikšanai asins plazmā. Gukālova B., zinātniskā vadītāja Dr. chem. Grīnberga S. Maģistra darbs, 52 lappuses, 12 attēli, 11 tabulas, 7 pielikumi, 56 literatūras avoti. Latviešu valodā. Darbā apkopota literatūra par LC-MS/MS pielietošanas iespējām otrās izvēles prettuberkulozes zāļu vielu un to metabolītu kvantitatīvai noteikšanai, raksturotas bioloģisko paraugu sagatavošanas metodes. Darba gaitā tika pārbaudītas dažādas paraugu sagatavošanas metodes, veikta hromatogrāfisko un masspektrometrisko apstākļu pielāgošana kvantitatīvai prettuberkulozes zāļu un to metabolītu noteikšanai asins plazmā.…

ω-Hetarilbutānskābes atvasinājumu iegūšana pretvēža aktivitātes noteikšanai: pieclocekļu heterocikli ar trim heteroatomiem.

2022

ω-Hetarilbutānskābes atvasinājumu iegūšana pretvēža aktivitātes noteikšanai: pieclocekļu heterocikli ar trim heteroatomiem. Fiļipčuks M., zinātniskais vadītājs Dr. chem. Kļimenkovs I. Bakalaura darbs, 37 lappuses, 35 attēli, 1 tabula, 33 literatūras avoti, 19 pielikumi. Latviešu valodā. Bakalaura darba ietvaros tika izpētītas un aprakstītas diaizvietotu pieclocekļu heterociklisko savienojumu ar trim heteroatomiem iegūšanas iespējas.

Ar 4-(dimetilamino)piridīnu modificētas ceolītveidīgās imidazola karkasa struktūras sintēze

2017

Ar (4-dimetilamino)piridīnu modificētas ceolītveidīgās karkasa struktūras sintēze. Vasiļevska A., zinātniskie vadītāji prof., Dr. chem. Sūna E.; Mg. chem. Klūga R. Bakalaura darbs, 30 lappuses, 31 attēls, 1 tabula, 37 literatūras avoti. Latviešu valodā. Darbā ir veikta potenciāla ar DMAP modificēta ZIF organiskā liganda 4-fluor-3-(1Himidazol- 2-il)piridīna sintēze. Literatūras apskatā aplūkota informācija par 2-arilimidazolu sintēzes metodēm un piridīna fluorēšanas iespējām. 2-IMIDAZOLĪNA SINTĒZE, FLUORĒŠANA, DIAZOTĒŠANA, PIRIDĪNS, ZIF, DMAP

DICATIONIC IMIDAZOLIUM SALTS: TUNABLE ANTIMICROBIAL AND ANTITUMORAL CHEMIOTHERAPEUTIC LEADS

2015

The chemical synthesis of novel chemotherapeutical leads is evolving thanks to possibility to design molecules with desired physical-chemical and, thus, biological properties. The imidazolium salts, recently proven effective to inhibit bacterial and/or cancer cell growth, posses an amphiphilic nature that is conferred by the imidazolium cation having a polar head generally coupled with aliphatic side chains. Thus, biological properties of imidazolium salts can be tuned through modifications involving the cation structure and/or the anion nature. By covalently linking two imidazolium rings, di-imidazolium salts were obtainedobtain differing in: i) kind of anions; ii) geometric isomerization …

Oksazolonu iegūšana no hidroksilgrupu saturošām aminoskābēm

2018

Oksazolonu iegūšana no hidroksilgrupu saturošām aminoskābēm. Kokars D., zinātniskais vadītājs doc. Igors Kļimenkovs. Bakalaura darbs, 51 lapaspuse, 13 attēli, 1 tabulas, 17 literatūras avoti, 15 pielikumi. Latviešu valodā. Darbā ir aplūkotas atbilstošo oksazolonu iegūšanas metodes no DL-serīna un DL-tirozīna. Darbā dažādos apstākļos tiek veikta aminoskābju N-acilēšana, pēc kuras tiek iegūti atbilstošie oksazoloni, izmantojot T3P un DCC. Lai pārliecinātos par produkta identitāti un tīrību, tiek uzņemts 1H-KMR spektrs un IS spektrs.

Influences of histone deacetylase inhibitors and resveratrol on DNA repair and chromatin compaction

2013

Accessibility of DNA is a prerequisite for both DNA damage and repair. Therefore, the chromatin structure is expected to have major impact on both processes, with opposite consequences for the stability of the genome. To analyse the influence of chromatin compaction on the generation and repair of various types of DNA modifications, we modulated the global chromatin structure of AS52 Chinese hamster ovary cells and HeLa cells by treatment with either histone deacetylase inhibitors or resveratrol and measured the repair kinetics of (i) pyrimidine dimers induced by ultraviolet B, (ii) oxidised purines generated by photosensitisation and (iii) single-strand breaks induced by H2O2, using an alk…