Search results for "affinity"

F-type lectin from the sea bass (Dicentrarchus labrax): purification, cDNA cloning, tissue expression and localization, and opsonic activity.

2009

Recently described biochemical and structural aspects of fucose-binding lectins from the European eel (Anguilla anguilla) and striped bass (Morone saxatilis) led to the identification of a novel lectin family ("F-type" lectins) characterized by a unique sequence motif and a characteristic structural fold. The F-type fold is shared not only with other members of this lectin family, but also with apparently unrelated proteins ranging from prokaryotes to vertebrates. Here we describe the purification, biochemical and molecular properties, and the opsonic activity of an F-type lectin (DlFBL) isolated from sea bass (Dicentrarchus labrax) serum. DlFBL exhibits two tandemly arranged carbohydrate-r…

Different sample treatment approaches for the analysis of T-2 and HT-2 toxins from oats-based media.

2010

A LC-DAD method is proposed for the determination of the T-2 and HT-2 toxins in cultures of Fusarium langsethiae in oat-based and other in vitro media. Test media consisted of freshly prepared milled oats to which T-2 and HT-2 toxin stock solutions were added. Different mixtures of extraction solvent (acetonitrile:water and methanol water), extraction times (30', 60' or 90') and drying methods were investigated. Results showed that extraction with methanol: water (80:20, v/v) for 90 min, drying with N-2 and subsequent analysis by LC-DAD was the fastest and most user friendly method for detecting HT-2 and T-2 toxins production by F. langsethiae strains grown on oat-based media at levels of 0…

Study of the morphological affinity between two species of benthic foraminifera from restricted environments: Rosalina douvillei (Cushman, 1928) and …

2020

A biometric comparison is carried out on selected tests of the foraminiferal species Rosalina douvillei (Cushman, 1928) and Trichohyalus aguayoi (Bermudez, 1935). The Rosalina douvillei specimens were recovered from the marginal marine Oligocene/Miocene site of Russingen (Mainz Basin, Germany), the lacustrine beds of the lower Miocene site of Bunol (Valencian Community, Spain) and the middle/ upper Miocene site of Fuendetodos (Aragon, Spain). The Trichohyalus aguayoi tests were collected in the current coastal lagoon of Torreblanca and recovered from Holocene cores sampled in the Peniscola marsh and in the coastal lagoon of l’Albufera de Valencia, all them in the Valencian Community (Spain)…

The oocyte derived growth factors, GDF9 and GDF9B, and their biological activities in 'in vitro' cell models

2007

Plato and Antisthenes in the Phaedo: A Reflexive Reading. Part One

2019

The purpose of this study is not so much to show the presence of Antisthenes in the dialogue, but rather to examine what Plato alludes to. The controversy over ideas between the two Socratics is historically very well-attested, as can already be seen in the Cratylus. Thus, it is reasonable to assume that this controversy must have affected Plato when he was writing a dialogue in which the importance of ideas and his new logic is undeniable. Hence, this paper will investigate the following question: what impact could Antisthenes’ denominative and definitory logic have on the equally denominative and definitory logic presented in the Phaedo given that the latter work in all probability preced…

Novel supramolecular affinity materials based on (−)-isosteviol as molecular templates

2013

The readily available ex-chiral-pool building block (−)-isosteviol was combined with the C3-symmetric platforms hexahydroxytriphenylene and hexaaminotriptycene providing large and rigid molecular architectures. Because of the persistent cavities these scaffolds are very potent supramolecular affinity materials for head space analysis by quartz crystal microbalances. The scaffolds serve in particular as templates for tracing air-borne arenes at low concentration. The affinities of the synthesized materials towards different air-borne arenes were determined by 200 MHz quartz crystal microbalances.

Sulle orme del consumatore affinity analysis e knowledge visualization per il processo decisionale

2016

L’articolo analizza i reali comportamenti di acquisto dell’acquirente all’interno del punto vendita e individua i pattern latenti più significativi attraverso un’analisi di affinità di 128.364 scontrini estratti da un dataset di 715.662 transazioni effettuate in un anno. Lo studio individua le strutture associative più ricorrenti relativamente alla singola merceologia. Attraverso la tecnica della Market Basket Analysis è stato possibile individuare le categorie merceologiche che creano “poli d’attrazione” sul punto vendita stimolando acquisti di altre categorie. In particolar modo, applicando le regole associative sul punto vendita è stato possibile identificare le “aree calde” e le “aree f…

Immunoaffinity purification and characterization of mitochondrial membrane-bound D-3-hydroxybutyrate dehydrogenase from Jaculus orientalis.

2008

Abstract Background The interconversion of two important energy metabolites, 3-hydroxybutyrate and acetoacetate (the major ketone bodies), is catalyzed by D-3-hydroxybutyrate dehydrogenase (BDH1: EC 1.1.1.30), a NAD+-dependent enzyme. The eukaryotic enzyme is bound to the mitochondrial inner membrane and harbors a unique lecithin-dependent activity. Here, we report an advanced purification method of the mammalian BDH applied to the liver enzyme from jerboa (Jaculus orientalis), a hibernating rodent adapted to extreme diet and environmental conditions. Results Purifying BDH from jerboa liver overcomes its low specific activity in mitochondria for further biochemical characterization of the e…

Physicochemical Properties of A New PEGylated Polybenzofulvene Brush for Drug Encapsulation

2019

A new polymer brush was synthesized by spontaneous polymerization of benzofulvene macromonomer 6-MOEG-9-T-BF3k bearing a nona(ethylene glycol) side chain linked to the 3-phenylindene scaffold by means of a triazole heterocycle. The polymer structure was studied by SEC-MALS, NMR spectroscopy, and MALDI-TOF MS techniques, and the results supported the role of oligomeric initiatory species in the spontaneous polymerization of polybenzofulvene derivatives. The aggregation features of high molecular weight poly-6-MOEG-9-T-BF3k-FE were investigated by pyrene fluorescence analysis, dynamic light scattering studies, and transmission electron microscopy, which suggested a tendency towards the format…

The affinities of monoclonal antibodies against core antigen of hepatitis B virus

1994

Four monoclonal antibodies generated against the recombinant core antigen of hepatitis B virus are investigated for antigen binding. All exhibit a similar affinity to polystyrene-sorbed antigen but only one of them interacts with native form of HBcAg (an assembled particle) in solution. The presence of 0.1% sodium dodecylsulphate is required for the binding of other three antibodies. The phenomenon can be interpreted as inaccessibility of the corresponding epitopes unless the multimeric antigen structure is disrupted. The core antigen coated on polystyrene is considered as a similar exposed structure.