Search results for "alanine"
showing 10 items of 499 documents
CCDC 1035539: Experimental Crystal Structure Determination
2014
Related Article: P. Lenartowicz, M. Makowski, B. Zarychta, K. Ejsmont|2014|Acta Crystallogr.,Sect.E:Struct.Rep.Online|70|596|doi:10.1107/S1600536814025677
CCDC 862035: Experimental Crystal Structure Determination
2017
Related Article: Dawid Siodłak, Agnieszka Macedowska-Capiga, Małgorzata A. Broda, Anna E. Kozioł, Tadeusz Lis|2012|Biopolymers|98|466|doi:10.1002/bip.22082
CCDC 192132: Experimental Crystal Structure Determination
2003
Related Article: B.Rzeszotarska, D.Siodlak, M.A.Broda, I.Dybala, A.E.Koziol|2002|J.Pept.Res.|59|79|doi:10.1034/j.1399-3011.2002.10951.x
Pyrrolomycins as antimicrobial agents. Microwave-assisted organic synthesis and insights into their antimicrobial mechanism of action
2019
Abstract New compounds able to counteract staphylococcal biofilm formation are needed. In this study we investigate the mechanism of action of pyrrolomycins, whose potential as antimicrobial agents has been demonstrated. We performed a new efficient and easy method to use microwave organic synthesis suitable for obtaining pyrrolomycins in good yields and in suitable amount for their in vitro in-depth investigation. We evaluate the inhibitory activity towards Sortase A (SrtA), a transpeptidase responsible for covalent anchoring in Gram-positive peptidoglycan of many surface proteins involved in adhesion and in biofilm formation. All compounds show a good inhibitory activity toward SrtA, havi…
Differences in metabolic profiles of planktonic and biofilm cells in Staphylococcus aureus - (1)H Nuclear Magnetic Resonance search for candidate bio…
2013
Staphylococcus aureus is responsible for many types of infections related to biofilm presence. As the early diagnostics remains the best option for prevention of biofilm infections, the aim of the work presented was to search for differences in metabolite patterns of S. aureus ATCC6538 biofilm vs. free-swimming S. aureus planktonic forms. For this purpose, Nuclear Magnetic Resonance (NMR) spectroscopy was applied. Data obtained were supported by means of Scanning Electron Microscopy, quantitative cultures and X-ray computed microtomography. Metabolic trends accompanying S. aureus biofilm formation were found using Principal Component Analysis (PCA). Levels of isoleucine, alanine and 2,3-but…
Crystal structure of N-(tert-butoxycarbonyl)phenylalanyldehydroalanine isopropyl ester (Boc–Phe–ΔAla–OiPr)
2014
In the crystal structure of the dehydrodipeptide (Boc-Phe-ΔAla-OiPr), the molecule has a trans configuration of the N-methylamide group. Its geometry is different from saturated peptides but is in excellent agreement with other dehydroalanine compounds. In the crystal, an N—H⋯O hydrogen bond links the molecules in a herringbone packing arrangement.
Surface-exposed Amino Acid Residues of HPV16 L1 Protein Mediating Interaction with Cell Surface Heparan Sulfate
2007
Efficient infection of cells by human papillomaviruses (HPVs) and pseudovirions requires primary interaction with cell surface proteoglycans with apparent preference for species carrying heparan sulfate (HS) side chains. To identify residues contributing to virus/cell interaction, we performed point mutational analysis of the HPV16 major capsid protein, L1, targeting surface-exposed amino acid residues. Replacement of lysine residues 278, 356, or 361 for alanine reduced cell binding and infectivity of pseudovirions. Various combinations of these amino acid exchanges further decreased cell attachment and infectivity with residual infectivity of less than 5% for the triple mutant, suggesting …
ALANINE/EPR DOSIMETRY APPLIED TO THE VALIDATION OF A TOTAL BODY IRRADIATION (TBI) PROTOCOL AND TREATMENT PLANNING DOSE CALCULATION USING A HUMANOID P…
2015
L’irraggiamento a corpo intero (Total Body Irradiation - TBI) è una tecnica di radioterapia oncologica di largo impiego nella clinica ematoncologica nell’ambito del trattamento di pazienti avviati al trapianto di midollo osseo o al trapianto di cellule staminali periferiche. La TBI da una parte permette di sopprimere il sistema immunitario del ricevente per prevenire il rigetto del midollo del donatore ed eliminare le cellule neoplastiche residue dai trattamenti chemioterapici. In genere, la modalità di somministrazione della TBI prevede irraggiamenti in 3 giorni consecutivi con un bi-frazionamento giornaliero (2 Gy, 2 volte al giorno con intervallo minimo di 6 ore tra le sedute, per 3 gior…
EPR DOSIMETRY WITH ALANINE FOR TOTAL BODY IRRADIATION USING AN ANTHROPOMORPHIC PHANTOM
2016
The Total Body Irradiation (TBI) is a radiation therapy technique widely used in clinical hemato-oncological treatment of patients that underwent bone marrow transplantation or peripheral stem cell transplantation. Typically, the administration of TBI treatment provides irradiations in 3 consecu-tive days with a double daily fractionation, although other dose fractionation modes have been widely adopted in the clinic procedures. Although this tech-nique allows to irradiate the whole body from head to toes, it has some limitations such as the lack of homogeneity of the dose distribution (which can show varia-tions of 20% in the different areas), irradiation of critical organs such as lungs, …
Effect of a novel chemical mixture on senescence processes and plant--fungus interaction in Solanaceae plants.
2001
The effects of exogenous application of a chemical mixture consisting of adipic acid monoethyl ester, furfurylamine, and 1,2,3,4-tetra-O-acetyl-beta-D-glucopyranose (FGA) on various metabolic pathways and the plant-fungus interaction have been studied in Solanaceae plants. Tomato and pepper plants were sprayed with the FGA mixture, and different biochemical parameters such as gas exchange, chlorophyll concentration, protein, cell wall sugar and phenolics contents, and peroxidase and phenylalanine ammonia lyase (PAL) activities were measured. FGA-treated plants showed, in general, an increase in cell wall sugar content and decreases in the chlorophyll degrading rate and the peroxidase activi…