Search results for "aldolase"
showing 10 items of 14 documents
Computational strategies for the design of new enzymatic functions
2015
In this contribution, recent developments in the design of biocatalysts are reviewed with particular emphasis in the de novo strategy. Studies based on three different reactions, Kemp elimination, Diels–Alder and Retro-Aldolase, are used to illustrate different success achieved during the last years. Finally, a section is devoted to the particular case of designed metalloenzymes. As a general conclusion, the interplay between new and more sophisticated engineering protocols and computational methods, based on molecular dynamics simulations with Quantum Mechanics/Molecular Mechanics potentials and fully flexible models, seems to constitute the bed rock for present and future successful desig…
Fructose-1,6-Bisphosphate Protects Hippocampal Rat Slices from NMDA Excitotoxicity
2019
Effects of fructose 1,6-bisphosphate (F-1,6-P2) towards N-methyl-d-aspartate NMDA excitotoxicity were evaluated in rat organotypic hippocampal brain slice cultures (OHSC) challenged for 3 h with 30 &mu
Identification of antigenic proteins from Echinostoma caproni (Trematoda) recognized by mouse immunoglobulins M, A and G using an immunoproteomic app…
2008
Antigenic proteins of Echinostoma caproni (Trematoda) against mouse IgM, IgA, IgG, IgG1 and IgG2a were investigated by immunoproteomics. Excretory/secretory products (ESP) of E. caproni separated by two-dimensional (2D) gel electrophoresis were transferred to nitrocellulose membranes and probed with the different mouse immunoglobulin classes. A total of four proteins (enolase, 70 kDa heat-shock protein (HSP-70), actin and aldolase) were accurately identified. Enolase was recognized in eight different spots of which seven of them were detected in the expected molecular weight and were recognized by IgA, IgG or IgG and IgG1. Another spot identified as enolase at 72 kDa was only recognized by …
Expression of Phosphofructokinase Is Not Sufficient to Enable Embden-Meyerhof-Parnas Glycolysis in Zymomonas mobilis ZM4
2019
Zymomonas mobilis is a bacterium that produces ethanol from glucose at up to 97% of theoretical efficiency on a carbon basis. One factor contributing to the high efficiency of ethanol production is that Z. mobilis has a low biomass yield. The low biomass yield may be caused partly by the low ATP yield of the Entner-Doudoroff (ED) glycolytic pathway used by Z. mobilis, which produces only one ATP per glucose consumed. To test the hypothesis that ATP yield limits biomass yield in Z. mobilis, we attempted to introduce the Embden-Meyerhof-Parnas (EMP) glycolytic pathway (with double the ATP yield) by expressing phosphofructokinase (Pfk I) from Escherichia coli. Expression of Pfk I caused growth…
Identification of proteins in excretory/secretory extracts of Echinostoma friedi (Trematoda) from chronic and acute infections.
2006
In the present study, we describe the investigation of Echinostoma friedi excretory/secretory products using a proteomic approach combined with the use of heterologous antibodies. We have identified 18 protein spots corresponding to ten proteins, including cytoskeletal proteins like actin, tropomyosin, and paramyosin; glycolytic enzymes like enolase, glyceraldehyde 3P dehydrogenase, and aldolase; detoxifying enzymes like GSTs; and stress proteins like heat shock protein (Hsp) 70. Among these proteins, both actin and, to a lesser extent, Hsp70, exhibited differential expression patterns between chronic and acute infections in the Echinostoma-rodent model, suggesting that these proteins may p…
Changes in enzymes involved in photosynthesis and other metabolic processes in the fruit of Opuntia ficus-indica during growth and ripening
2011
The aim of this study was to investigate changes in the abundance of a number of enzymes in the peel, core and seeds of fruits of Opuntia ficus-indica (L.) Miller during development. The enzymes studied were phosphoenolpyruvate carboxylase (PEPC; EC: 4.1.1.31), ribulose-1,5-bisphosphate carboxylase/oxygenase (RUBISCO; EC: 4.1.1.39), aldolase (EC: 4.1.2.13), pyruvate, orthophosphate dikinase (PPDK; EC: 2.7.9.1), phosphoenolpyruvate carboxykinase (PEPCK; EC: 4.1.1.49) and aspartate aminotransferase (AspAT; EC: 2.6.1.1). To detect these enzymes, antibodies specific for each enzyme were used to probe Western blots of sodium dodecyl sulphate polyacrylamide gels. Fruit weight increased throughout…
Aktivit�tsbestimmung der Isoenzyme der Aldolase im menschlichen Serum mit Hilfe pr�zipitierender Antik�rper
1975
Die Isoenzyme der Aldolase im Serum wurden mit Hilfe prazipitierender Antikorper bestimmt. Das Isoenzymmuster bei 130 gesunden Personen, aus einem Normwertprogramm ausgewahlt, ergab folgende Werte: Gesamt-Aldolase 0,8, 1,6, 2,5 U/L, Isoenzym A 0,6, 1,2, 1,9 U/L, Isoenzym B 0,0, 0,2 0,7 U/L und Isoenzym C 0,0, 0,1, 0,4 U/L ausgedruckt als $$\bar x$$ ±2s. Der Vergleich der Histogramme zeigt, das die Werteverteilung bei Messung der Gesamtaldolase sich bei Gesunden und Hepatitis-Kranken uberschneidet. Bei Messung der Aldolase B kommt es zu keiner Uberschneidung der Werteverteilung. Eine Differenzierung zwischen Gesunden und Kranken mit akuter Hepatitis ist moglich.
Hydrophobically Directed Aldol Reactions: Polystyrene-SupportedL-Proline as a Recyclable Catalyst for Direct Asymmetric Aldol Reactions in the Presen…
2007
The cover picture shows the aldol reaction between ketones and arylaldehydes carried out by using a polystyrene-supported L-proline catalyst. This material furnishes aldol products in high yields and stereoselectivities. Screening of solvents showed that these reactions take place only in the presence of water. This solvent effect, coupled with the high stereoselectivities observed, has been explained by the formation of a hydrophobic core in the inner surface of the resin with the hydrophilic proline moiety in the resin/water interface. Such a microenvironment promotes the aldol reaction and increases the stereoselectivity. Recycling investigations have shown that this material can be reus…
Zur frage: Psoriasis vulgaris und Leber
1964
Der wiederholt geauserten Meinung einer Krankheitskorrelation zwischen Psoriasis vulgaris und Leber wird durch Bestimmung fur die Leberdiagnostik besonders geeigneter Enzyme (Glutaminsaure-Oxalessigsaure-Transaminase, Glutaminsaure-Brenztraubensaure-Transaminase, Aldolase) und des Zweifarbstofftests sowie wiederholt auch des Serumgesamtbilirubins nachgegangen. In der uberwiegenden Zahl der Psoriasis vulgaris-Kranken konnten normale Befunde erhoben werden. Von 14 Psoriatikern mit vorwiegend nur gering von der Norm abweichenden Befunden konnte anamnestisch und klinisch zumeist die Ursache fur die pathologischen Serum veranderungen ermittelt werden, ohne das sich jedoch hieraus der Nachweis fu…
Chemoenzymatische „Chiral-Pool”-Synthese von (+)-exo-Brevicomin aus Kohlenhydraten mit Fructose-1,6-diphosphat-Aldolase
1990
Chemoenzymatic “Chiral-Pool” Synthesis of (+)-exo-Brevicomin from Carbohydrates Using Fructose 1,6-Diphosphate Aldolase Fructose-1,6-diphosphate aldolase (EC 4.1.2.13) catalyzes the stereospecific aldol reaction between 1,3-dihydroxyacetone phosphate (4) and 5-oxohexanal (3) or its 5-dithiane-protected analog 8. The products of the aldol reactions are dephosphorylated with acid phosphatase (EC 3.1.3.2). Whereas the aldol adduct of 3 cyclizes spontaneously to give the bicyclic brevicomin precursor 3, the adduct of 8 first has to be deprotected with sulfuryl chloride and wet silica gel. The resulting bicyclic α-hydroxy ketone 3 is reduced with LiAlH4 to form the 1,2-diol 14 which is then deox…