Search results for "ammonium"
showing 10 items of 1070 documents
A New Method to Reconstruct Quantitative Food Webs and Nutrient Flows from Isotope Tracer Addition Experiments
2020
Understanding how nutrients flow through food webs is central in ecosystem ecology. Tracer addition experiments are powerful tools to reconstruct nutrient flows by adding an isotopically enriched element into an ecosystem and tracking its fate through time. Historically, the design and analysis of tracer studies have varied widely, ranging from descriptive studies to modeling approaches of varying complexity. Increasingly, isotope tracer data are being used to compare ecosystems and analyze experimental manipulations. Currently, a formal statistical framework for analyzing such experiments is lacking, making it impossible to calculate the estimation errors associated with the model fit, the…
Growth and macromolecular content of the dimorphic fungus Aureobasidium pullulans and the effect of hydroxyurea and other inhibitors
1988
The growth kinetics and the macromolecular content of the yeast and ethanol-induced hyphal forms of Aureobasidium pullulans were studied. During the morphological transition from yeasts to hyphae, both the protein and RNA content decreased significantly, the mycelial form containing only 76% of the amount of protein in the yeasts, and 38% of the RNA. The DNA was the only component tested whose level increased during the transition. Among several compounds inhibiting macromolecular synthesis, only hydroxyurea showed a remarkable effect on the morphology of A. pullulans, inducing the mycelial morphology. The macromolecular composition of hydroxyurea-treated cultures changed with time in a way…
Comprehensive assessment of the microalgae-nitrifying bacteria competition in microalgae-based wastewater treatment systems: Relevant factors, evalua…
2022
[EN] Due to their capacity to assimilate carbon dioxide and nutrients, microalgae-based cultivation systems have emerged as a green solution for intensive wastewater treatment. However, when large concentrations of ammonium are present the competition between microalgae and ammonium-oxidising bacteria plays a significant role. Microalgae use ammonium to synthesise proteins, photosynthetic pigments and nucleic acids, while ammonium-oxidising bacteria use it as a source of electrons and oxidise it to nitrite.Several authors have studied the isolated factors that influence microalgae-nitrifying bacteria competition, although a comprehensive analysis of this interesting topic is still lacking. …
Monitoring pH and ORP in a SHARON reactor
2011
This paper analyses the valuable information provided by the on-line measurements of pH and oxidation reduction potential (ORP) in a continuous single high ammonia removal over nitrite (SHARON) reactor. A laboratory-scale SHARON reactor equipped with pH, ORP, electric conductivity and dissolved oxygen (DO) probes has been operated for more than one year. Nitrogen removal over nitrite has been achieved by adding methanol at the beginning of anoxic stages. Time evolution of pH and ORP along each cycle allows identifying the decrease in nitritation rate when ammonia is consumed during the aerobic phase and the end of the denitrification process during the anoxic phase. Therefore, monitoring pH…
Nitrite inhibition of microalgae induced by the competition between microalgae and nitrifying bacteria
2020
[EN] Outdoor microalgae cultivation systems treating anaerobic membrane bioreactor (AnMBR) effluents usually present ammonium oxidising bacteria (AOB) competition with microalgae for ammonium uptake, which can cause nitrite accumulation. In literature, nitrite effects over microalgae have shown controversial results. The present study evaluates the nitrite inhibition role in a microalgae-nitrifying bacteria culture. For this purpose, pilot- and lab-scale assays were carried out. During the continuous outdoor operation of the membrane photobioreactor (MPBR) plant, biomass retention time (BRT) of 2 d favoured AOB activity, which caused nitrite accumulation. This nitrite was confirmed to inhib…
Effect of pH and HNO2 concentration on the activity of ammonia-oxidizing bacteria in a partial nitritation reactor
2013
Ammonia-oxidizing bacteria (AOB) are very sensitive to environmental conditions and wastewater treatment plant operational parameters. One of the most important factors affecting their activity is pH. Its effect is associated with: NH3/NH4 þ and HNO2/NO2 chemical equilibriums and biological reaction rates. The aim of this study was to quantify and model the effect of pH and free nitrous acid (FNA) concentration on the activity of AOB present in a lab-scale partial nitritation reactor. For this purpose, two sets of batch experiments were carried out using biomass from this reactor. Fluorescent in situ hybridization analysis showed that Nitrosomona eutropha and Nitrosomona europaea species we…
Production of microalgal external organic matter in a Chlorella-dominated culture: influence of temperature and stress factors
2020
[EN] Although microalgae are recognised to release external organic matter (EOM), little is known about this phenomenon in microalgae cultivation systems, especially on a large scale. A study on the effect of microalgae-stressing factors such as temperature, nutrient limitation and ammonium oxidising bacteria (AOB) competition in EOM production by microalgae was carried out. The results showed non-statistically significant differences in EOM production at constant temperatures of 25, 30 and 35 degrees C. However, when the temperature was raised from 25 to 35 degrees C for 4 h a day, polysaccharide production increased significantly, indicating microalgae stress. Nutrient limitation also see…
Selecting the most suitable microalgae species to treat the effluent from an anaerobic membrane bioreactor.
2018
[EN] Conventional treatments for nutrient removal in wastewater are shifting to Anaerobic Membrane Bioreactors, which produce a high-quality effluent with minimum sludge production. The effluent resulting contains high nitrogen and phosphorus load that can be eliminated by microalgae culture. The aim of this study is to evaluate the ammonium and phosphorus removal rate of different microalgae species in the effluent of an anaerobic treatment. For that, 4 different microalgae species have been tested (Chlamydomonas reinhardtii, Scenedesmus obliquus, Chlorella vulgaris and Monoraphidium braunii) in batch monoculture and mixed conditions. Results indicate that all species are able to eliminate…
In Situ Ammonium Profiling Using Solid-Contact Ion-Selective Electrodes in Eutrophic Lakes
2015
A promising profiling setup for in situ measurements in lakes with potentiometric solid-contact ion-selective electrodes (SC-ISEs) and a data processing method for sensor calibration and drift correction are presented. The profiling setup consists of a logging system, which is equipped with a syringe sampler and sensors for the measurement of standard parameters including temperature, conductivity, oxygen and photosynthetically active radiation (PAR). The setup was expanded with SC-ISEs in galvanically separated amplifiers. The potential for high-resolution profiling is investigated by deploying the setup in the eutrophic Lake Rotsee (Lucerne, Switzerland), using two different designs of am…
NQS-Doped PDMS Solid Sensor: From Water Matrix to Urine Enzymatic Application
2021
The development of in situ analytical devices has gained outstanding scientific interest. A solid sensing membrane composed of 1,2-naphthoquinone-4-sulfonate (NQS) derivatizing reagent embedded into a polymeric polydimethylsiloxane (PDMS) composite was proposed for in situ ammonium (NH4+) and urea (NH2CONH2) analysis in water and urine samples, respectively. Satisfactory strategies were also applied for urease-catalyzed hydrolysis of urea, either in solution or glass-supported urease immobilization. Using diffuse reflectance measurements combined with digital image processing of color intensity (RGB coordinates), qualitative and quantitative analyte detection was assessed after the colorime…